scholarly journals Emergence ofKlebsiella pneumoniaeCoproducing KPC-2 and 16S rRNA Methylase ArmA in Poland

2010 ◽  
Vol 55 (1) ◽  
pp. 443-446 ◽  
Author(s):  
Katarzyna Zacharczuk ◽  
Katarzyna Piekarska ◽  
Jolanta Szych ◽  
Elwira Zawidzka ◽  
Agnieszka Sulikowska ◽  
...  
Keyword(s):  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Variable Region ◽  
Pulsed Field Gel Electrophoresis ◽  
Epidemic Strain ◽  
Pulsed Field ◽  
Ca 50 ◽  
16S Rrna Methylase

ABSTRACTAKlebsiella pneumoniaeepidemic strain that coproduced carbapenemase KPC-2 (K. pneumoniaecarbapenemase 2) and 16S rRNA methylase ArmA has emerged in Poland. Four nonduplicate isolates from patients in a hospital in Warsaw, Poland, were found to carry theblaKPC-2andarmAgenes on ca. 50-kb and 90-kb plasmids, respectively. Tn4401with a 100-bp deletion in the variable region was detected in all the isolates. XbaI pulsed-field gel electrophoresis (PFGE) revealed 93.2% similarity of the isolates. All the isolates were resistant to carbapenems and 4,6-disubstituted 2-deoxystreptamines.

scholarly journals Plasmid-Mediated 16S rRNA Methylases in Aminoglycoside-Resistant Enterobacteriaceae Isolates in Shanghai, China

2008 ◽  
Vol 53 (1) ◽  
pp. 271-272 ◽  
Author(s):  
Qiong Wu ◽  
Yibo Zhang ◽  
Lizhong Han ◽  
Jingyong Sun ◽  
Yuxing Ni
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
High Level ◽  
Level Resistance

ABSTRACT High-level resistance to aminoglycosides produced by 16S rRNA methylases in Enterobacteriaceae isolates was investigated. The prevalences of armA in Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae were 0.6%, 3.0%, and 10%, respectively. rmtB was more prevalent than armA. Pulsed-field gel electrophoresis patterns indicated that armA and rmtB have spread horizontally and clonally.

scholarly journals Identification of 16S rRNA Methylase-Producing Acinetobacter baumannii Clinical Strains in North America

2007 ◽  
Vol 51 (11) ◽  
pp. 4209-4210 ◽  
Author(s):  
Yohei Doi ◽  
Jennifer M. Adams ◽  
Kunikazu Yamane ◽  
David L. Paterson
Keyword(s):  
North America ◽  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Gel Electrophoresis ◽  
Medical Center ◽  
Pulsed Field Gel Electrophoresis ◽  
Aminoglycoside Resistance ◽  
Pulsed Field ◽  
Clinical Strains ◽  
16S Rrna Methylase

ABSTRACT Five highly amikacin-resistant Acinetobacter baumannii isolates were collected at a medical center in Pennsylvania. The aminoglycoside resistance was due to the production of the 16S rRNA methylase ArmA. Two of the isolates coproduced OXA-23 β-lactamase and were highly resistant to carbapenems as well. The isolates were genetically closely related by pulsed-field gel electrophoresis.

Moxifloxacin Therapy as a Risk Factor forClostridium difficile–Associated Disease During an Outbreak: Attempts to Control a New Epidemic Strain

2007 ◽  
Vol 28 (2) ◽  
pp. 198-201 ◽  
Author(s):  
Priscilla Biller ◽  
Beth Shank ◽  
Leah Lind ◽  
Meghan Brennan ◽  
Lisa Tkatch ◽  
...  
Keyword(s):  
Risk Factor ◽  
Clostridium Difficile ◽  
North American ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Epidemic Strain ◽  
Pulsed Field

An outbreak ofClostridium difficile-associated disease (CDAD) caused by the epidemic North American pulsed-field gel electrophoresis type 1 (NAP1) strain began after a formulary change from levofloxacin to moxifloxacin. Cases of CDAD were associated with moxifloxacin use, but a formulary change back to levofloxacin failed to reduce rates of disease. Substituting use of one fluoroquinolone with use of another without also controlling the overall use of drugs from this class is unlikely to control outbreaks caused by the NAP1 strain ofC. difficile.

scholarly journals Clinical Isolates of Enterobacteriaceae Producing Extended-Spectrum β-Lactamases: Prevalence of CTX-M-3 at a Hospital in China

2003 ◽  
Vol 47 (2) ◽  
pp. 790-793 ◽  
Author(s):  
Hui Wang ◽  
Swathi Kelkar ◽  
Weiyuan Wu ◽  
Minjun Chen ◽  
John P. Quinn
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Citrobacter Freundii ◽  
Pulsed Field ◽  
Extended Spectrum

ABSTRACT The prevalence of extended-spectrum β-lactamase-producing strains was demonstrated in 5 of 44 (11.4%) Escherichia coli, 17 of 43 (39.5%) Klebsiella pneumoniae, 3 of 50 (6.0%) Enterobacter cloacae, and 2 of 25 (8.0%) Citrobacter freundii strains at a teaching hospital in China. Nineteen of these 27 strains expressed CTX-M-3 β-lactamase (pI 8.6). A subset of the clinical isolates expressing the CTX-M-3 enzyme, tested by pulsed-field gel electrophoresis, revealed multiple clones. Five isolates expressed a novel enzyme, SHV-43 (pI 8.0), which had two substitutions (Leu113Phe and Thr149Ser) compared with SHV-1.

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