scholarly journals Antifungal Resistance of Candida glabrata Vaginal Isolates and Development of a Quantitative Reverse Transcription-PCR-Based Azole Susceptibility Assay

2008 ◽  
Vol 52 (9) ◽  
pp. 3424-3426 ◽  
Author(s):  
Scott E. Gygax ◽  
John-Paul Vermitsky ◽  
Sean G. Chadwick ◽  
Matthew J. Self ◽  
Jessica A. Zimmerman ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Gold Standard ◽  
Candida Glabrata ◽  
Fold Increase ◽  
Opportunistic Pathogen ◽  
Azole Resistance ◽  
Pcr Assay ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr ◽  
Susceptibility Assay

ABSTRACT A multiplex quantitative reverse transcription-PCR assay was developed to detect azole resistance in Candida glabrata, an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a ≥3-fold increase in CDR1 expression by this assay, which proved to be 100% sensitive and 95% specific in comparison to the gold standard broth microdilution assay.

scholarly journals Comparison of Automated Quantitative Reverse Transcription-PCR and Direct Fluorescent-Antibody Detection for Routine Rabies Diagnosis in the United States

2015 ◽  
Vol 53 (9) ◽  
pp. 2983-2989 ◽  
Author(s):  
Michelle Dupuis ◽  
Scott Brunt ◽  
Kim Appler ◽  
April Davis ◽  
Robert Rudd
Keyword(s):  
United States ◽  
Reverse Transcription ◽  
Gold Standard ◽  
Fluorescent Antibody ◽  
The United States ◽  
Pcr Assay ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Direct Fluorescent Antibody ◽  
Rabies Diagnosis

Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunction with quantitative reverse transcription-PCR (qRT-PCR), to augment or potentially replace the DFA test, was the focus of this project. Here we describe a triplex qRT-PCR assay, including assembly and evaluation for sensitivity, specificity, and ability to detect variants. Additionally, we compared the qRT-PCR assay to the gold standard direct fluorescent-antibody test. More than 1,000 specimens submitted for routine rabies diagnosis were tested to directly compare the two methods. All results were in agreement between the two methods, with one additional specimen detected by qRT-PCR below the limits of the DFA sensitivity. With the proper continued validation for variant detection, molecular methods have a place in routine rabies diagnostics within the United States.

Real-time quantitative reverse transcription-PCR assay for renal cell carcinoma-associated antigen G250

2002 ◽  
Vol 318 (1-2) ◽  
pp. 33-40 ◽  
Author(s):  
Ye Chuanzhong ◽  
Guan Ming ◽  
Zhang Fanglin ◽  
Chen Haijiao ◽  
Lin Zhen ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Real Time ◽  
Reverse Transcription ◽  
Renal Cell ◽  
Pcr Assay ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

scholarly journals Quantitative Reverse Transcription-PCR Assay for Detection of mRNA Encoding Full-Length Human Tissue Kallikrein 7: Prognostic Relevance of KLK7 mRNA Expression in Breast Cancer 3

2006 ◽  
Vol 52 (6) ◽  
pp. 1070-1079 ◽  
Author(s):  
Leon Holzscheiter ◽  
Julia C Biermann ◽  
Matthias Kotzsch ◽  
Panagiotis Prezas ◽  
Juliane Farthmann ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mrna Expression ◽  
Reverse Transcription ◽  
Human Tissue ◽  
Full Length ◽  
Tissue Kallikrein ◽  
Pcr Assay ◽  
Cancer Disease ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

Abstract Background: The human tissue kallikrein gene family (KLK1 to KLK15) encodes a group of 15 serine proteases (hK1 to hK15), several of which have been implicated in cancer-related processes. Methods: We established a specific quantitative reverse transcription-PCR assay for full-length KLK7 mRNA that excluded amplification of the exon 2 deletion splice variant (the latter does not encode a functional protease), and evaluated full-length KLK7 mRNA expression [normalized to human glucose-6-phosphate dehydrogenase (h-G6PDH)] in tumor tissue specimens from 155 breast cancer patients. Results: High KLK7 mRNA expression (continuous) was significantly associated with a better patient outcome according to both univariate (P = 0.005) and multivariate (P = 0.046) Cox survival analysis. Separation of patients by optimized dichotomization revealed a significantly better prognosis for patients with high KLK7 mRNA status (n = 89) compared with patients with low KLK7 mRNA status (n = 66) [univariate hazard ratio (HR) = 0.45 (P = 0.001); multivariate HR = 0.50 (P = 0.005)]. In the subgroup of patients not receiving adjuvant treatment (n = 69), KLK7 mRNA status was a significant prognosticator [univariate HR = 0.29 (P = 0.002); multivariate HR = 0.40 (P = 0.034)]. This subgroup was least influenced by postoperative treatment and thus best showed the impact of KLK7 expression on the natural course of breast cancer disease. Conclusion: Expression of full-length KLK7 mRNA may represent a new prognostic marker in breast cancer disease.

scholarly journals Implementation of an in-house real-time reverse transcription-PCR assay to detect the emerging SARS-CoV-2 N501Y variants

2021 ◽  
pp. 104868
Author(s):  
Marielle BEDOTTO ◽  
Pierre-Edouard FOURNIER ◽  
Linda HOUHAMDI ◽  
Philippe COLSON ◽  
Didier RAOULT
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Pcr Assay ◽  
Reverse Transcription Pcr
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