scholarly journals Quantitative Reverse Transcription-PCR Assay for Detection of mRNA Encoding Full-Length Human Tissue Kallikrein 7: Prognostic Relevance of KLK7 mRNA Expression in Breast Cancer 3

2006 ◽  
Vol 52 (6) ◽  
pp. 1070-1079 ◽  
Author(s):  
Leon Holzscheiter ◽  
Julia C Biermann ◽  
Matthias Kotzsch ◽  
Panagiotis Prezas ◽  
Juliane Farthmann ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mrna Expression ◽  
Reverse Transcription ◽  
Human Tissue ◽  
Full Length ◽  
Tissue Kallikrein ◽  
Pcr Assay ◽  
Cancer Disease ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

Abstract Background: The human tissue kallikrein gene family (KLK1 to KLK15) encodes a group of 15 serine proteases (hK1 to hK15), several of which have been implicated in cancer-related processes. Methods: We established a specific quantitative reverse transcription-PCR assay for full-length KLK7 mRNA that excluded amplification of the exon 2 deletion splice variant (the latter does not encode a functional protease), and evaluated full-length KLK7 mRNA expression [normalized to human glucose-6-phosphate dehydrogenase (h-G6PDH)] in tumor tissue specimens from 155 breast cancer patients. Results: High KLK7 mRNA expression (continuous) was significantly associated with a better patient outcome according to both univariate (P = 0.005) and multivariate (P = 0.046) Cox survival analysis. Separation of patients by optimized dichotomization revealed a significantly better prognosis for patients with high KLK7 mRNA status (n = 89) compared with patients with low KLK7 mRNA status (n = 66) [univariate hazard ratio (HR) = 0.45 (P = 0.001); multivariate HR = 0.50 (P = 0.005)]. In the subgroup of patients not receiving adjuvant treatment (n = 69), KLK7 mRNA status was a significant prognosticator [univariate HR = 0.29 (P = 0.002); multivariate HR = 0.40 (P = 0.034)]. This subgroup was least influenced by postoperative treatment and thus best showed the impact of KLK7 expression on the natural course of breast cancer disease. Conclusion: Expression of full-length KLK7 mRNA may represent a new prognostic marker in breast cancer disease.

scholarly journals Application of a Multigene Reverse Transcription-PCR Assay for Detection of Mammaglobin and Complementary Transcribed Genes in Breast Cancer Lymph Nodes

2002 ◽  
Vol 48 (8) ◽  
pp. 1225-1231 ◽  
Author(s):  
Barbara K Zehentner ◽  
Davin C Dillon ◽  
Yuqiu Jiang ◽  
Jiangchun Xu ◽  
Angela Bennington ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Mrna Expression ◽  
Reverse Transcription ◽  
Breast Tumor ◽  
Metastatic Breast ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

Abstract Background: Mammaglobin mRNA expression is found in 70–80% of primary and metastatic breast tumor biopsies. The potential breast tumor markers B305D, B726P, and γ-aminobutyrate type A receptor π subunit (GABAπ) complement the expression of mammaglobin. Collectively the expression profile of these four genes could be used as a diagnostic and prognostic indicator for breast cancer. Methods: A multigene reverse transcription-PCR (RT-PCR) assay was established to detect the expression of mammaglobin, GABAπ, B305D, and B726P simultaneously. Specific primers and TaqMan® probes were used to analyze combined mRNA expression profiles in primary breast tumors and metastatic lymph node specimens. Results: The multigene RT-PCR assay detected substantial expression signals in 27 of 27 primary tumor and 50 of 50 metastatic breast lymph node samples. Specificity studies demonstrated no significant expression signal in 27 non-breast cancer lymph nodes, in 22 various healthy tissue samples, or in 14 colon tumor samples. Conclusion: The novel RT-PCR-based assay described here provides a sensitive detection system for disseminated breast tumor cells in lymph nodes. In addition, this multigene assay could also be used to test peripheral blood and bone marrow samples.

Real-time quantitative reverse transcription-PCR assay for renal cell carcinoma-associated antigen G250

2002 ◽  
Vol 318 (1-2) ◽  
pp. 33-40 ◽  
Author(s):  
Ye Chuanzhong ◽  
Guan Ming ◽  
Zhang Fanglin ◽  
Chen Haijiao ◽  
Lin Zhen ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Real Time ◽  
Reverse Transcription ◽  
Renal Cell ◽  
Pcr Assay ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

Development of real-time quantitative reverse transcription-PCR for Her2 detection in peripheral blood from patients with breast cancer

2007 ◽  
Vol 384 (1-2) ◽  
pp. 52-56 ◽  
Author(s):  
Maria Savino ◽  
Maria Garrubba ◽  
Paola Parrella ◽  
Filomena Baorda ◽  
Massimiliano Copetti ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Reverse Transcription ◽  
Peripheral Blood ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

scholarly journals Mammaglobin as a Novel Breast Cancer Biomarker: Multigene Reverse Transcription-PCR Assay and Sandwich ELISA

2004 ◽  
Vol 50 (11) ◽  
pp. 2069-2076 ◽  
Author(s):  
Barbara K Zehentner ◽  
David H Persing ◽  
Amadou Deme ◽  
Papa Toure ◽  
Stephen E Hawes ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Reverse Transcription ◽  
Sandwich Elisa ◽  
Clinical Staging ◽  
Diagnostic Tools ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Blood Samples ◽  
Mixed Control ◽  
Reverse Transcription Pcr

Abstract Background: The aim of this study was to examine the potential usefulness of a mammaglobin multigene reverse transcription-PCR (RT-PCR) assay and a mammaglobin sandwich ELISA as diagnostic tools in breast cancer. Methods: We studied peripheral blood samples from 147 untreated Senegalese women with biopsy-confirmed breast cancer and gathered patient information regarding demographic, and clinical staging of disease. The samples were tested for mammaglobin and three breast cancer-associated gene transcripts by a multigene real-time RT-PCR assay and for serum mammaglobin protein by a sandwich ELISA assay. Results: In 77% of the breast cancer blood samples, a positive signal was obtained in the multigene RT-PCR assay detecting mammaglobin and three complementary transcribed genes. Fifty samples from healthy female donors tested negative. Significant correlations were found between mammaglobin protein in serum, presence of mammaglobin mRNA-expressing cells in blood, stage of disease, and tumor size. Circulating mammaglobin protein was detected in 68% of the breast cancer sera, and was increased in 38% in comparison with a mixed control population. The RT-PCR assay and the ELISA for mammaglobin produced a combined sensitivity of 84% and specificity of 97%. Conclusion: The ELISA and RT-PCR for mammaglobin and mammaglobin-producing cells could be valuable tools for diagnosis and prognosis of breast cancer.

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