scholarly journals Complex Class 1 Integrons with Diverse Variable Regions, Including aac(6′)-Ib-cr, and a Novel Allele, qnrB10, Associated with ISCR1 in Clinical Enterobacterial Isolates from Argentina

2007 ◽  
Vol 51 (12) ◽  
pp. 4466-4470 ◽  
Author(s):  
María Paula Quiroga ◽  
Patricia Andres ◽  
Alejandro Petroni ◽  
Alfonso J. C. Soler Bistué ◽  
Leonor Guerriero ◽  
...  
Keyword(s):  
Variable Region ◽  
Quinolone Resistance ◽  
The Novel ◽  
Complex Class ◽  
Unique Region ◽  
Variable Regions ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Novel Allele

ABSTRACT Transferable quinolone resistance has not previously been reported in Argentina. Here we describe three complex class 1 integrons harboring the novel allele qnrB10 in a unique region downstream of orf513, one of them also containing aac(6′)-Ib-cr within the variable region of integrons. The three arrays differed from bla CTX-M-2-bearing integrons, which are broadly distributed in Argentina.

scholarly journals Molecular Basis of Sulfonamide and Trimethoprim Resistance in Fish-Pathogenic Aeromonas Isolates

2011 ◽  
Vol 77 (20) ◽  
pp. 7147-7150 ◽  
Author(s):  
Kristina Kadlec ◽  
Ellen von Czapiewski ◽  
Heike Kaspar ◽  
Jürgen Wallmann ◽  
Geovana Brenner Michael ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Molecular Basis ◽  
Aeromonas Salmonicida ◽  
The Novel ◽  
Content Type ◽  
Gene Cassettes ◽  
Class 1 Integrons ◽  
Novel Gene ◽  
Class 1 ◽  
Diseased Fish

ABSTRACTSulfonamide-trimethoprim-resistantAeromonas salmonicidaand motileAeromonasspp. from diseased fish of the GERM-Vet study carried thesul1gene together with mostly cassette-borne trimethoprim resistance genes, including the novel genedfrA28. The sevendfrAanddfrBgenes identified were located mostly in class 1 integrons which commonly harbored other gene cassettes.

Salmonella entericaserovarTyphimurium Virulence-Resistance Plasmids Derived from the pSLT Carrying Nonconventional Class 1 Integrons withdfrA12Gene in Their Variable Region andsul3in the 3′ Conserved Segment

2013 ◽  
Vol 19 (6) ◽  
pp. 437-445 ◽  
Author(s):  
Janine Beutlich ◽  
M. Rosario Rodicio ◽  
M. Carmen Mendoza ◽  
Patricia García ◽  
Miranda Kirchner ◽  
...  
Keyword(s):  
Variable Region ◽  
Class 1 Integrons ◽  
Resistance Plasmids ◽  
Class 1

scholarly journals VIM-2-Producing Pseudomonas spp. in Uruguay: Sequence Types, Pulsotypes, and Class 1 Integrons Including New Variable Regions FeaturingblaVIM-2andblaGES-7

2016 ◽  
Vol 60 (9) ◽  
pp. 5620-5622 ◽  
Author(s):  
Romina Papa Ezdra ◽  
Inés Bado ◽  
Nicolás Cordeiro ◽  
Claudia Gutierrez ◽  
Patricia Hitateguy ◽  
...  
Keyword(s):  
Variable Regions ◽  
Class 1 Integrons ◽  
Pseudomonas Spp ◽  
Class 1 ◽  
Sequence Types

scholarly journals Identification and characterization of two novel ISCR1-associated genes dfrA42 and dfrA43 encoding trimethoprim resistant dihydrofolate reductases

2021 ◽  
Author(s):  
Ling Li ◽  
Mengge Zhang ◽  
Wenjia Wang ◽  
Ruirui Xia ◽  
Yanan Ma ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Complex Class ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Dihydrofolate Reductases ◽  
High Level ◽  
Identification And Characterization

Two novel ISCR1-associated dfr genes, dfrA42 and dfrA43, were identified from trimethoprim (TMP)-resistant Proteus strains and were shown to confer high level TMP resistance (MIC ≥ 1024 mg/L) when cloned into Escherichia coli. These genes were hosted by complex class 1 integrons suggesting their potentials for dissemination. Analysis of enzymatic parameters and TMP affinity were performed, suggesting that the mechanism of TMP resistance for these novel DHFRs is the reduction of binding with TMP.

scholarly journals Expression of the aac(6′)-Ib-cr Gene in Class 1 Integrons

2017 ◽  
Vol 61 (5) ◽  
Author(s):  
Sophie Raherison ◽  
Thomas Jove ◽  
Margaux Gaschet ◽  
Emilie Pinault ◽  
Aurore Tabesse ◽  
...  
Keyword(s):  
Amino Acids ◽  
Resistance Gene ◽  
Gene Cassette ◽  
Quinolone Resistance ◽  
Start Codon ◽  
Class 1 Integrons ◽  
Alternative Start Codon ◽  
Class 1

ABSTRACT aac(6′)-Ib-cr is a plasmid-mediated quinolone resistance gene embedded within a gene cassette, most often within an integron. It confers resistance to quinolones and aminoglycosides. We investigated the role of a 101-bp fragment frequently present upstream of the aac(6′)-Ib-cr gene cassette and found that it contributes to the expression of aac(6′)-Ib-cr and provides an alternative start codon, confirming the length of the AAC(6′)-Ib-cr protein to 199 amino acids.

scholarly journals Antimicrobial Resistance and Spread of Class 1 Integrons among Salmonella Serotypes

2000 ◽  
Vol 44 (8) ◽  
pp. 2166-2169 ◽  
Author(s):  
Beatriz Guerra ◽  
Sara Soto ◽  
Santiago Cal ◽  
M. Carmen Mendoza
Keyword(s):  
Multidrug Resistance ◽  
Resistance Genes ◽  
Antimicrobial Agents ◽  
Resistance Pattern ◽  
Variable Regions ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Salmonella Serotypes ◽  
Reference Strains ◽  
Spanish Region

ABSTRACT The resistance profiles, for 15 antimicrobial agents, of 333Salmonella strains representing the most frequent nontyphoidal serotypes, isolated between 1989 and 1998 in a Spanish region, and 9 reference strains were analyzed. All strains were susceptible to amikacin, ceftazidime, ciprofloxacin, and imipenem, and 31% were susceptible to all antimicrobials tested. The most frequent types of resistance were to sulfadiazine, tetracycline, streptomycin, spectinomycin, ampicillin, and chloramphenicol (ranging from 46 to 22%); 13% were resistant to these six drugs. This multidrug resistance pattern was found alone or together with other resistance types within serotypes Typhimurium (45%), Panama (23%), and Virchow (4%). Each isolate was also screened for the presence of class 1 integrons and selected resistance genes therein; seven variable regions which carried one (aadA1a, aadA2, orpse-1) or two (dfrA14-aadA1a,dfrA1-aadA1a, oxa1-aadA1a, orsat1-aadA1a) resistance genes were found in integrons.

scholarly journals Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil

2015 ◽  
Vol 9 (08) ◽  
pp. 890-897 ◽  
Author(s):  
Milena Dropa ◽  
Livia Carminato Balsalobre ◽  
Nilton Lincopan ◽  
Glavur Rogério Matté ◽  
Maria Helena Matté
Keyword(s):  
Complex Class ◽  
Wild Type ◽  
Beta Lactamases ◽  
E Coli ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Extended Spectrum Beta Lactamases ◽  
Genetic Structures ◽  
Hospital Wards ◽  
Type Strains

Introduction: CTX-M enzymes are the most prevalent extended-spectrum beta-lactamases (ESBLs) in Brazil and around the world. The spread of CTX-M lies in their ability to be mobilized by insertion sequences and integrons. This study aimed to identify the mobile genetic structures associated with blaCTX-M genes from clinical Enterobacteriaceae strains. Methodology: Twenty-eight clinical non-clonal Enterobacteriaceae were screened by PCR for the presence of blaCTX-M genes and class 1 integrase (int1), and for the association of blaCTX-M with class 1 integrons. Plasmid incompatibility groups were assessed by PBRT. Wild-type plasmids were transformed into electrocompetent E. coli, and the S1-PFGE technique was used to verify the presence of high-molecular-weight plasmids in both wild-type strains and E. coli transformants. Results: Sequencing showed that strains carried blaCTX-M-2 (n = 25) and blaCTX-M-59 (n = 3) genes inserted into the 3’-end of complex class 1 integrons. Thirteen strains also carried blaTEM and blaSHV genes. CTX-M-2/59-containing complex class 1 integrons were also present in E. coli transformants. The most frequent Inc groups were IncA/C (n = 10) and IncF (n = 8). Heavy plasmids were observed in both wild-type strains and E. coli transformants. Conclusions: The presence of the same blaCTX-M-2-group-containing genetic structure in seven Enterobacteriaceae species isolated at seven hospital wards shows the great mobility potential of complex class 1 integrons. Also, this is the first report of TEM-15, SHV-45, and SHV-55 in Latin America. The genetic environment of blaCTX-M-2 accounts for their maintenance and spread among Gram-negative bacteria.

scholarly journals Prevalence and Genetic Analysis of Plasmid-Mediated Quinolone Resistance Determinants QnrA and QnrS in Enterobacteriaceae Isolates from a French University Hospital

2006 ◽  
Vol 50 (12) ◽  
pp. 3992-3997 ◽  
Author(s):  
Laurent Poirel ◽  
Cécile Leviandier ◽  
Patrice Nordmann
Keyword(s):  
Genetic Analysis ◽  
Nalidixic Acid ◽  
Insertion Sequence ◽  
Quinolone Resistance ◽  
University Hospital ◽  
Class 1 Integrons ◽  
Resistance Determinants ◽  
Class 1 ◽  
Resistant Strains ◽  
Lactamase Gene

ABSTRACT The spread of plasmid-mediated quinolone resistance determinants QnrA and QnrS was evaluated in a collection of 186 extended-spectrum β-lactamase (ESBL)-positive enterobacterial isolates from 2002 to 2005 and 185 nalidixic acid-resistant strains isolated during the first 6 months of 2005 at the Bicêtre hospital, France. Out of these 186 ESBL-positive isolates, 2.2 and 1.6% carried a QnrA1 and a QnrS1 determinant, respectively. The ESBLs associated with QnrA1 were VEB-1, SHV-12, and CTX-M-1, whereas those associated with QnrS1 were TEM-52, SHV-12, and CTX-M-1. Among the 185 nalidixic acid-resistant strains isolated in 2005, 0.5 and 2.7% had a QnrA1 determinant and a QnrS1 determinant, respectively. The genetic environments of the qnrA1 gene differed but were always associated with sul1 type integrons. In contrast, qnrS1 genes were not embedded in class 1 integrons but located often (but not systematically) downstream of the insertion sequence ISEcl2 on plasmids that often carried a novel β-lactamase gene, bla LAP-1. This is the first study identifying the QnrS resistance determinant in Europe and indicating that this determinant might also be widespread.

Detection of blaCTX-M-type genes in complex class 1 integrons carried by Enterobacteriaceae isolated from retail chicken meat in Brazil

2015 ◽  
Vol 197 ◽  
pp. 88-91 ◽  
Author(s):  
Tiago Casella ◽  
María Margarita Rodríguez ◽  
Juliana Tiemi Takahashi ◽  
Barbara Ghiglione ◽  
Milena Dropa ◽  
...  
Keyword(s):  
Chicken Meat ◽  
Complex Class ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Retail Chicken Meat
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