Development of an Intracellular Screen for New Compounds Able To Inhibit Mycobacterium tuberculosis Growth in Human Macrophages
2015 ◽
Vol 60
(1)
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pp. 640-645
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Keyword(s):
ABSTRACTHere we describe the development and validation of an intracellular high-throughput screening assay for finding new antituberculosis compounds active in human macrophages. The assay consists of a luciferase-based primary identification assay, followed by a green fluorescent protein-based secondary profiling assay. Standard tuberculosis drugs and 158 previously recognized active antimycobacterial compounds were used to evaluate assay robustness. Data show that the assay developed is a short and valuable tool for the discovery of new antimycobacterial compounds.
2012 ◽
Vol 18
(2)
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pp. 191-198
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2010 ◽
Vol 8
(6)
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pp. 656-668
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2002 ◽
Vol 70
(10)
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pp. 5800-5807
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2014 ◽
Vol 19
(9)
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pp. 1266-1274
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2019 ◽
2006 ◽
Vol 4
(1)
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pp. 64-67
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2018 ◽
Vol 20
(9)
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pp. 804-819
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