Comparative activities of amikacin against Mycobacterium avium complex in nude and beige mice.

After 4 weeks of treatment, clarithromycin (CLAR) and amikacin showed similar antimicrobial activities against the Mycobacterium avium complex in mice. There was a difference, however, in the effectiveness of the drugs in different types of mice: both drugs displayed bactericidal effects in beige mice but only bacteriostatic effects in nude mice. Because the effectiveness of CLAR is less in nude mice than in beige mice, the predictive value of the nude mouse model for the efficacy of chemotherapy is less than that of the beige mouse model.

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A Nude Mouse Model as an in vivo Infectivity Assay for Cryptospomdiosis

Water Science & Technology ◽

10.2166/wst.1993.0323 ◽

1993 ◽

Vol 27 (3-4) ◽

pp. 65-68 ◽

Cited By ~ 3

Author(s):

B. H. Kwa ◽

M. Moyad ◽

M. A. Pentella ◽

J. B. Rose

Keyword(s):

Drinking Water ◽

Mouse Model ◽

Nude Mouse ◽

Cryptosporidium Parvum ◽

Nude Mice ◽

Surface Waters ◽

Nude Mouse Model ◽

Infectivity Assay ◽

Limiting Dilution

Cryptosporidium parvum is an important patliogen of diarrlieal disease which has been implicated in several outbreaks associated with contamination of surface waters. In monitoring for C. parvum in drinking water sources, it is important to asce tain the viability, and more importantly, the infectivity of low numbers of recovered oocysts. Groups of 10 Balb/C nude (nu/nu) mice, 4-8 weeks old at time of inoculation, were infected with C. parvum oocysts from naturally infected calves and purified using Sheather's sucrose gradients. Oocysts were counted using the Merifluor IFA kit (Meridian). Each group of 10 mice were infected with 1,10,100 and 1000 oocysts respectively. Numbers of oocysts per inoculation were determined by limiting dilution, and parallel inocula were counted microscopically to ascertain the accuracy of the dilutions. Two uninfected nude mice were kept in each cage to serve as controls. Mouse stools were collected every 4 days, concentrated using the Fekal Kontrate Concentration Kit (Meridian) and oocysts were counted with a UV microscope using the Merifluor IFA Kit (Meridian). Oocyst counts were expressed in terms of number of oocyst/g feces. Mice inoculated with 1000 oocysts began to shed oocysts on day 32, mice inoculated with 100 oocysts began to shed on days 44-48, mice inoculated with 10 oocysts began to shed on days 56-60, and mice inoculated with 1 oocyst shed on days 68-88. All infected mice continued to shed oocysts intermittently and with variable oocyst counts until day 180 when the experiment was terminated. This study established that it is possible to infect nude mice with very low numbers, down to a single oocyst. We are currently in the process of correlating the nude mouse assay with other viability assays.

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Analysis of Development Mechanism of Giant Cell Arteritis in Nude Mouse Model through Color Duplex Sonography and Computerized Tomography Nanocontrast Agent

BioMed Research International ◽

10.1155/2021/6627925 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Fugang Chen ◽

Yang Li ◽

Hongyan Zhou ◽

Chuang Sun ◽

Sun Li ◽

...

Keyword(s):

Mouse Model ◽

Nude Mouse ◽

Nude Mice ◽

Temporal Artery ◽

Duplex Sonography ◽

The Body ◽

Control Group ◽

Color Duplex Sonography ◽

Nude Mouse Model ◽

Experimental Group

To explore the application value of color duplex sonography and enhanced computerized tomography (CT) inspection based on a nanocontrast agent in diagnosis and pathogenesis in giant cell arteritis (GCA), the GCA nude mouse model was constructed. In this study, 40 healthy male BalB/c nude mice aged 6-8 weeks were randomly divided into a control group (no model) and an experimental group (model), with 20 mice in each group, and the temporal artery tissue of GCA patients diagnosed as positive by temporal artery biopsy was implanted into nude mice to construct a GCA nude mouse model. Abdominal aortic biopsy and immunohistochemistry were used to verify the success of the GCA nude mouse model. All nude mice were subjected to color duplex sonography and enhanced CT examination based on a nanocontrast agent. At the same time, the basic indicators such as body weight, temperature, white blood cell (WBC), lymphocytes (LYM), hemoglobin (HGB), and platelet (PLT) were measured, and the protein expression levels of interleukin-6 (IL-6) and signal transducer and activator of transcription 3 (STAT3) were detected by immunohistochemistry. The results showed that the temporal artery wall of the nude mice in the experimental group thickened and the lumen was significantly narrowed, indicating that the cell arteritis model of nude mice was successfully constructed; ultrasound examination showed that the right superficial temporal artery vascular cavity narrowed, the blood flow signal changed like a filling defect around the periphery, and there was a low echo halo. CT examination showed that the left superficial temporal artery narrowed, and the inner diameter of the narrow segment of blood vessels changed like a bead. The body weight of nude mice in the experimental group decreased significantly after the modeling was completed ( P < 0.05 ); after modeling, the body temperature of the nude mice in the experimental group increased significantly ( P < 0.05 ); LYM and HGB values of nude mice in the experimental group were significantly lower than those in the control group ( P < 0.05 ); the content of IL-6, STAT3, IL-6, and STAT3 proteins in the arterial tissue of nude mice in the experimental group was lower than that of the control group ( P < 0.05 ), indicating that color duplex sonography and CT contrast agent technology can be used in the diagnosis and development mechanism research of GC.

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