scholarly journals Rifampin Augments Cytokine-Induced Nitric Oxide Production in Human Alveolar Epithelial Cells

2006 ◽  
Vol 50 (1) ◽  
pp. 396-398 ◽  
Author(s):  
Yael Yuhas ◽  
Eva Berent ◽  
Hila Ovadiah ◽  
Inbar Azoulay ◽  
Shai Ashkenazi
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Alveolar Epithelial Cells ◽  
Nitric Oxide Production ◽  
Alveolar Cells ◽  
Alveolar Epithelial ◽  
Oxide Production ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide ◽  
Human Alveolar Epithelial Cells

ABSTRACT Rifampin increased nitric oxide production and inducible nitric oxide synthase expression in alveolar cells stimulated with cytokines. Nitric oxide concentrations after induction with cytokines, cytokines with 10 μg/ml rifampin, and cytokines with 50 μg/ml rifampin were 3.2, 4.5, and 8.8 μM, respectively (P < 0.02 versus cytokines alone). This indicates that rifampin modulates the immune response.

scholarly journals Modulation of in vivo alloreactivity by inhibition of inducible nitric oxide synthase.

1995 ◽  
Vol 181 (1) ◽  
pp. 63-70 ◽  
Author(s):  
N K Worrall ◽  
W D Lazenby ◽  
T P Misko ◽  
T S Lin ◽  
C P Rodi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Biologically Active ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

The role of nitric oxide in the immune response to allogeneic tissue was explored in an in vivo cardiac transplant model in the rat. Nitric oxide production during organ rejection was demonstrated by elevations in systemic serum nitrite/nitrate levels and by electron paramagnetic resonance spectroscopy. Messenger RNA for the inducible nitric oxide synthase enzyme was detected in the rejecting allografted heart, but not in the nonrejecting isografted heart. The enzyme was demonstrated to be biologically active by the in vitro conversion of L-arginine to L-citrulline and was immunohistochemically localized to the infiltrating inflammatory cells. Treatment with aminoguanidine, a preferential inhibitor of the inducible nitric oxide synthase isoform, prevented the increased nitric oxide production in the transplanted organ and significantly attenuated the pathogenesis of acute rejection. Aminoguanidine treatment prolonged graft survival, improved graft contractile function, and significantly reduced the histologic grade of rejection. These results suggest an important role for nitric oxide in mediating the immune response to allogeneic tissue. Inhibition of inducible nitric oxide synthase may provide a novel therapeutic modality in the management of acute transplant rejection and of other immune-mediated processes.

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