scholarly journals Simultaneous anaerobic and aerobic ammonium and methane oxidation under oxygen limitation

2021 ◽  
Author(s):  
Maartje A.H.J. van Kessel ◽  
Karin Stultiens ◽  
Arjan Pol ◽  
Mike S.M. Jetten ◽  
Boran Kartal ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Methane Oxidation ◽  
Oxygen Limitation ◽  
Water Bodies ◽  
Anammox Bacteria ◽  
Fish Analysis ◽  
Potential Solution ◽  
Anammox Activity ◽  
Nitrite Nitrate ◽  
Receiving Water

Methane and ammonia have to be removed from wastewater treatment effluent in order to discharge it to receiving water bodies. A potential solution for this is a combination of simultaneous ammonia and methane oxidation by anaerobic ammonia-oxidizing (anammox) bacteria and nitrite/nitrate-dependent anaerobic methane oxidizing (N-damo) microorganisms. When applied, these microorganisms will be exposed to oxygen but little is known about the effect of a low concentration of oxygen on a culture containing these microorganisms. In this study, a stable co-culture containing anammox and N-damo microorganisms in a laboratory scale bioreactor was established under oxygen limitation. Membrane inlet mass spectrometry (MIMS) was used to directly measure the in situ simultaneous activity of N-damo, anammox and aerobic ammonia oxidizing microorganisms. In addition, batch tests revealed that the bioreactor also harbored aerobic methanotrophs and anaerobic methanogens. Together with FISH analysis and metagenomics, these results indicate that the combination of N-damo and anammox activity under the continuous supply of limiting oxygen concentrations is feasible and can be implemented for the removal of methane and ammonia from anaerobic digester effluents. Importance Nitrogen in wastewater leads to eutrophication of the receiving water bodies and methane is a potent greenhouse gas; it is therefore important that these are removed from wastewater. A potential solution for the simultaneous removal of nitrogenous compounds and methane is the application of a combination of nitrite/nitrate depended methane oxidation (N-damo) and anammox. In order to do so, it is important to investigate the effect of oxygen on these two anaerobic processes. In this study, we investigate the effect of a continuous oxygen supply on the activity of an anaerobic methane and ammonia oxidizing coculture. The findings presented in this study are important for the potential application of these two microbial processes in wastewater treatment.

scholarly journals Assessing carrying capacity of receiving water bodies – A case study in Southern waterways of Binh Duong province

2019 ◽  
Vol 2 (6) ◽  
pp. 84-97
Author(s):  
Le Ngoc Tuan ◽  
Tao Manh Quan ◽  
Tran Thi Thuy ◽  
Doan Thanh Huy ◽  
Tran Xuan Hoang
Keyword(s):  
Water Quality ◽  
Wastewater Treatment ◽  
Carrying Capacity ◽  
Water Quality Management ◽  
Pollution Source ◽  
Water Bodies ◽  
Source Control ◽  
Local Pollution ◽  
Surface Water Management ◽  
Receiving Water

The carrying capacity of receiving water bodies is one of the important data for water quality management, pollution source control towards harmonizing with the economic development and environment protection. Therefore, this research aimed atevaluating the carrying capacity of receiving water bodies in the south of Binh Duong province up to 2030. 06 key water quality indicators (COD, BOD, TSS, PO43--P, NO3--N, NH4+-N) were exmained with 02 wastewater treatment scenarios. Results showed the investigated area hardly had carrying capacity for NH4+-N and PO43--P, followed by TSS, BOD, and COD. In case of improving wastewater treatment status till 2030, the carrying capacity of receiving water bodies would be increased, but not significant. The carrying capacity of several basins needs to be paid special attention are: Suoi Con 1 basin (BOD, COD, NH4+-N), Suoi Cai basin (BOD, TSS and NH4+-N), the upstream of Cay Bang – Cau Dinh basin (BOD, COD, TSS, NH4+-N), the upstream of Chom Sao – Rach Bung basin (05 parameters, excepting NO3--N), the upstream of Binh Hoa – Vinh Binh basin (BOD, COD, PO43--P, NH4+-N). These findings are an important basis for formulating strategies and proposing measures for local pollution source control and surface water management.  

The occurrence of opioid compounds in wastewater treatment plants and their receiving water bodies in Gauteng province, South Africa

2021 ◽  
pp. 118048
Author(s):  
Ilunga Kamika ◽  
Shohreh Azizi ◽  
Adolph A. Muleja ◽  
Ramganesh Selvarajan ◽  
Mohamed Azab El-Liethy ◽  
...  
Keyword(s):  
South Africa ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Water Bodies ◽  
Gauteng Province ◽  
Receiving Water

Anammox bacteria enrichment and characterization from municipal activated sludge

2011 ◽  
Vol 64 (7) ◽  
pp. 1428-1434 ◽  
Author(s):  
J. C. Araujo ◽  
A. C. Campos ◽  
M. M. Correa ◽  
E. C. Silva ◽  
M. H. Matté ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Batch Reactor ◽  
Phase 1 ◽  
Treatment Plant ◽  
Ammonia Removal ◽  
Anammox Bacteria ◽  
Fish Analysis ◽  
Relative Population ◽  
N Concentration ◽  
Anammox Activity

A sustainable option for nitrogen removal is the anaerobic ammonium-oxidizing (anammox) process in which ammonium is oxidized to nitrogen gas with nitrite as electron acceptor. Application of this process, however, is limited by the availability of anammox biomass. In this study, two Brocadia-like anammox phylotypes were successfully enriched, detected and identified from an activated sludge taken from a domestic wastewater treatment plant (Minas Gerais, Brazil) employing a Sequencing Batch Reactor (SBR). The dominant phylotype was closely related to ‘Candidatus Brocadia sinica’, but one clone seemed to represent a novel species for which we propose the name ‘Candidatus Brocadia brasiliensis’. Based on Fluorescence in situ hybridization (FISH) analysis, this enrichment led to a relative population size of 52.7% (±15.6) anammox bacteria after 6 months of cultivation. The cultivation process can be divided into three phases: phase 1 (approximately 25 days) was characterized by heterotrophic denitrification metabolism, phase 2 was the propagation phase and phase 3 (from the 87th day onwards), in which significant anammox activity was detected. A long-term performance of the SBR showed a near perfect removal of nitrite based on the influent NO2−-N concentration of 61–95 mg L−1. The average ammonia removal efficiency was 90% with the influent NH4+-N concentration of 55–82 mg L−1. Therefore, anammox cultivation and enrichment from activated sludge was possible under a controlled environment within 3 months.

Tool for selecting indicator substances to evaluate the impact of wastewater treatment plants on receiving water bodies

2020 ◽  
Vol 745 ◽  
pp. 140746
Author(s):  
Ira Brückner ◽  
Silke Classen ◽  
Monika Hammers-Wirtz ◽  
Kassandra Klaer ◽  
Joachim Reichert ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Water Bodies ◽  
The Impact ◽  
Receiving Water

scholarly journals An Analysis of Operation Conditions and Microbial Characteristics in Swine Wastewater Treatment Plants with Spontaneously Enriched Anammox Bacteria

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1010
Author(s):  
Miyoko Waki ◽  
Chikako Ishimoto ◽  
Ryu Suto ◽  
Takafumi Nagamine ◽  
Toshimi Matsumoto ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Swine Wastewater ◽  
Continuous Reactor ◽  
Anammox Bacteria ◽  
Activity Levels ◽  
Operation Conditions ◽  
Microbial Characteristics ◽  
Anammox Activity ◽  
Treatment Facilities

The spontaneous enrichment of anammox bacteria has been reported in swine wastewater treatment facilities. However, their causative conditions and microbial characteristics, which this study aims to explain, are poorly understood. We discovered eight treatment facilities where the collected red biofilms exhibited high anammox activity levels at 57–843 µmol-N2/g-ignition loss (IL)/h and anammox DNA concentrations of 4.3 × 108–1.6 × 1012 copies/g-IL. The facilities used various wastewater treatment methods—six of them employed a multi-stage continuous reactor, whereas aeration tanks were continuously aerated at another combination of six facilities. Levels of dissolved oxygen (DO) in these tanks were fairly low at ≤1 mg/L. Pyrosequencing of the biofilms indicated the presence of 3–62.5% Planctomycetes, and the dominant anammox in each biofilm comprised three operational taxonomic units (OTUs) similar to Candidatus Jettenia asiatica, Ca. Brocadia fulgida, and Ca. B. caroliniensis. This suggested that some particular species of anammox bacteria naturally thrive when operating a swine wastewater treatment facility at low DO levels. The frequent enrichment of anammox biofilms at the sampled sites indicated that these treatment facilities were good seed sources of anammox; therefore, anammox treatment would be a viable method for the removal of nitrogen from swine wastewater.

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