scholarly journals Analysis of the Genome Sequence of Lactobacillus gasseri ATCC 33323 Reveals the Molecular Basis of an Autochthonous Intestinal Organism

2008 ◽  
Vol 74 (15) ◽  
pp. 4610-4625 ◽  
Author(s):  
M. Andrea Azcarate-Peril ◽  
Eric Altermann ◽  
Yong Jun Goh ◽  
Richard Tallon ◽  
Rosemary B. Sanozky-Dawes ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Native Species ◽  
Bacterial Species ◽  
Gc Content ◽  
Intestinal Epithelial ◽  
Growth And Survival ◽  
Lactobacillus Gasseri ◽  
Bacteriophage Infection ◽  
A Genome

ABSTRACT This study presents the complete genome sequence of Lactobacillus gasseri ATCC 33323, a neotype strain of human origin and a native species found commonly in the gastrointestinal tracts of neonates and adults. The plasmid-free genome was 1,894,360 bp in size and predicted to encode 1,810 genes. The GC content was 35.3%, similar to the GC content of its closest relatives, L. johnsonii NCC 533 (34%) and L. acidophilus NCFM (34%). Two identical copies of the prophage LgaI (40,086 bp), of the Sfi11-like Siphoviridae phage family, were integrated tandomly in the chromosome. A number of unique features were identified in the genome of L. gasseri that were likely acquired by horizontal gene transfer and may contribute to the survival of this bacterium in its ecological niche. L. gasseri encodes two restriction and modification systems, which may limit bacteriophage infection. L. gasseri also encodes an operon for production of heteropolysaccharides of high complexity. A unique alternative sigma factor was present similar to that of B. caccae ATCC 43185, a bacterial species isolated from human feces. In addition, L. gasseri encoded the highest number of putative mucus-binding proteins (14) among lactobacilli sequenced to date. Selected phenotypic characteristics that were compared between ATCC 33323 and other human L. gasseri strains included carbohydrate fermentation patterns, growth and survival in bile, oxalate degradation, and adhesion to intestinal epithelial cells, in vitro. The results from this study indicated high intraspecies variability from a genome encoding traits important for survival and retention in the gastrointestinal tract.

scholarly journals Draft Genome Sequence of Streptomyces mexicanus Strain Q0842, Isolated from Human Skin

2020 ◽  
Vol 9 (44) ◽  
Author(s):  
Manon Boxberger ◽  
Mariem Ben Khedher ◽  
Anthony Levasseur ◽  
Bernard La Scola
Keyword(s):  
Human Skin ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Bacterial Species ◽  
Draft Genome ◽  
Gc Content ◽  
Draft Genome Sequence ◽  
Swab Sample ◽  
Partial Genome Sequence ◽  
Partial Genome

ABSTRACT In 2003, Streptomyces mexicanus was reported as a novel xylanolytic bacterial species isolated from soil; a partial genome sequence was determined. In 2019, a strain from the same species was isolated from a hand skin swab sample from a healthy French woman. Genome sequencing revealed an 8,011,832-bp sequence with a GC content of 72.5%.

scholarly journals Draft Genome Sequence of Lactobacillus rhamnosus OSU-PECh-69, a Cheese Isolate with Antibacterial Activity

2020 ◽  
Vol 9 (37) ◽  
Author(s):  
Israel García-Cano ◽  
Walaa E. Hussein ◽  
Diana Rocha-Mendoza ◽  
Ahmed E. Yousef ◽  
Rafael Jiménez-Flores
Keyword(s):  
Genome Sequence ◽  
Antimicrobial Agents ◽  
Draft Genome ◽  
Lactobacillus Rhamnosus ◽  
Gc Content ◽  
Gene Clusters ◽  
Gram Negative Bacteria ◽  
The Novel ◽  
Content Type ◽  
A Genome

ABSTRACT The novel strain Lactobacillus rhamnosus OSU-PECh-69 was isolated from provolone cheese. It produces antimicrobial agents having a molecular mass of 5 to 10 kDa that are active against Gram-positive and Gram-negative bacteria. The strain has a genome sequence of 3,057,669 bp, a GC content of 46.6%, and up to two gene clusters encoding bacteriocins.

scholarly journals Novel metrics for quantifying bacterial genome composition skews

2017 ◽  
Author(s):  
Lena M. Joesch-Cohen ◽  
Max Robinson ◽  
Neda Jabbari ◽  
Christopher Lausted ◽  
Gustavo Glusman
Keyword(s):  
Gene Annotation ◽  
Bacterial Species ◽  
Bacterial Genome ◽  
Gc Content ◽  
Bacterial Genomes ◽  
Genome Composition ◽  
Single Genome ◽  
A Genome ◽  
Dna Strands ◽  
Interactive Visualizations

AbstractBackgroundBacterial genomes have characteristic compositional skews, which are differences in nucleotide frequency between the leading and lagging DNA strands across a segment of a genome. It is thought that these strand asymmetries arise as a result of mutational biases and selective constraints, particularly for energy efficiency. Analysis of compositional skews in a diverse set of bacteria provides a comparative context in which mutational and selective environmental constraints can be studied. These analyses typically require finished and well-annotated genomic sequences.ResultsWe present three novel metrics for examining genome composition skews; all three metrics can be computed for unfinished or partially-annotated genomes. The first two metrics, (dot-skew and cross-skew) depend on sequence and gene annotation of a single genome, while the third metric (residual skew) highlights unusual genomes by subtracting a GC content-based model of a library of genome sequences. We applied these metrics to all 7738 available bacterial genomes, including partial drafts, and identified outlier species. A number of these outliers (i.e., Borrelia, Ehrlichia, Kinetoplastibacterium, and Phytoplasma) display similar skew patterns despite only distant phylogenetic relationship. While unrelated, some of the outlier bacterial species share lifestyle characteristics, in particular intracellularity and biosynthetic dependence on their hosts.ConclusionsOur novel metrics appear to reflect the effects of biosynthetic constraints and adaptations to life within one or more hosts on genome composition. We provide results for each analyzed genome, software and interactive visualizations at http://db.systemsbiology.net/gestalt/skew_metrics.

scholarly journals Draft Genome Sequence of the UV-Resistant Antarctic Bacterium Sphingomonas sp. Strain UV9

2019 ◽  
Vol 8 (7) ◽  
Author(s):  
Juan J. Marizcurrena ◽  
Danilo Morales ◽  
Pablo Smircich ◽  
Susana Castro-Sowinski
Keyword(s):  
Genome Size ◽  
Genome Sequence ◽  
Draft Genome ◽  
Gc Content ◽  
Draft Genome Sequence ◽  
Protein Coding ◽  
Coding Sequences ◽  
Antarctic Bacterium ◽  
A Genome ◽  
The Antarctic

We report the draft genome sequence of the Antarctic UV-resistant bacterium Sphingomonas sp. strain UV9. The strain has a genome size of 4.25 Mb, a 65.62% GC content, and 3,879 protein-coding sequences.

scholarly journals Draft Genome Sequence of Lactobacillus mulieris UMB7784, Isolated from the Female Urinary Tract

2020 ◽  
Vol 9 (21) ◽  
Author(s):  
Oleksandra Tsibere ◽  
Taylor Miller-Ensminger ◽  
Adelina Voukadinova ◽  
Alan J. Wolfe ◽  
Catherine Putonti
Keyword(s):  
Urinary Tract ◽  
Genome Size ◽  
Genome Sequence ◽  
Draft Genome ◽  
Gc Content ◽  
Draft Genome Sequence ◽  
A Genome ◽  
Female Bladder ◽  
Female Urinary Tract

ABSTRACT We sequenced the genome of Lactobacillus mulieris UMB7784, isolated from the urinary tract. With a genome size of 1,695,489 bp and a GC content of 34.2%, the draft genome sequence presented here expands our understanding of lactobacilli in the female bladder.

scholarly journals Draft Genome Sequence of Lactobacillus paraplantarum OSY-TC318, a Producer of the Novel Lantibiotic Paraplantaracin TC318

2019 ◽  
Vol 8 (19) ◽  
Author(s):  
Walaa E. Hussein ◽  
En Huang ◽  
Ismet Ozturk ◽  
Ahmed E. Yousef
Keyword(s):  
Genome Size ◽  
Genome Sequence ◽  
Draft Genome ◽  
Gc Content ◽  
Peptide Sequencing ◽  
Draft Genome Sequence ◽  
The Novel ◽  
Content Type ◽  
A Genome ◽  
Lactobacillus Paraplantarum

Lactobacillus paraplantarum OSY-TC318 was isolated from Turkish Tulum cheese and found to produce a potent anti-Gram-positive peptide. Sequencing of the OSY-TC318 genome revealed a genome size of 3,587,488 bp and an average GC content of 43.4%.

scholarly journals De Novo Assembly of the Pneumocystis jirovecii Genome from a Single Bronchoalveolar Lavage Fluid Specimen from a Patient

mBio ◽  
2012 ◽  
Vol 4 (1) ◽  
Author(s):  
Ousmane H. Cissé ◽  
Marco Pagni ◽  
Philippe M. Hauser
Keyword(s):  
Bronchoalveolar Lavage ◽  
Genome Sequence ◽  
Bronchoalveolar Lavage Fluid ◽  
De Novo ◽  
Gc Content ◽  
Lavage Fluid ◽  
New Drugs ◽  
Pneumocystis Jirovecii ◽  
Content Type

ABSTRACTPneumocystis jiroveciiis a fungus that causes severe pneumonia in immunocompromised patients. However, its study is hindered by the lack of anin vitroculture method. We report here the genome ofP. jiroveciithat was obtained from a single bronchoalveolar lavage fluid specimen from a patient. The major challenge was thein silicosorting of the reads from a mixture representing the different organisms of the lung microbiome. This genome lacks virulence factors and most amino acid biosynthesis enzymes and presents reduced GC content and size. Together with epidemiological observations, these features suggest thatP. jiroveciiis an obligate parasite specialized in the colonization of human lungs, which causes disease only in immune-deficient individuals. This genome sequence will boost research on this deadly pathogen.IMPORTANCEPneumocystispneumonia is a major cause of mortality in patients with impaired immune systems. The availability of theP. jiroveciigenome sequence allows new analyses to be performed which open avenues to solve critical issues for this deadly human disease. The most important ones are (i) identification of nutritional supplements for development of culturein vitro, which is still lacking 100 years after discovery of the pathogen; (ii) identification of new targets for development of new drugs, given the paucity of present treatments and emerging resistance; and (iii) identification of targets for development of vaccines.

scholarly journals Draft Genome Sequence of Pseudomonas stutzeri Strain KMS 55, an Endophytic Diazotroph Isolated from Rice Roots

2017 ◽  
Vol 5 (40) ◽  
Author(s):  
Kannepalli Annapurna ◽  
Venkadasamy Govindasamy ◽  
Meenakshi Sharma ◽  
Yuvika Rajrana ◽  
Karivaradharajan Swarnalakshmi ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Genome Sequence ◽  
Draft Genome ◽  
Gc Content ◽  
Pseudomonas Stutzeri ◽  
Single Copy ◽  
23S Rrna ◽  
Protein Coding ◽  
Content Type ◽  
A Genome

ABSTRACT Pseudomonas stutzeri strain KMS 55 (MTCC 12703) is an isolate from the root tissues of rice (Oryza sativa L.) that displays a high biological nitrogen fixation ability. Here, we report the complete genome sequence of this strain, which contains 4,637,820 bp, 4,289 protein-coding genes, 5,006 promoter sequences, 62 tRNAs, a single copy of 5S-16S-23S rRNA, and a genome average GC content of 51.18%. Analysis of the ~4.64-Mb genome sequence will give support to increased understanding of the genetic determinants of host range, endophytic colonization behavior, endophytic nitrogen fixation, and other plant-beneficial roles of Pseudomonas stutzeri.

scholarly journals Draft Genome Sequence of Arthrobacter globiformis mrc11, an Antimicrobial Agent Isolated from a Khangkhui Cave Deposit

2019 ◽  
Vol 8 (11) ◽  
Author(s):  
Dinabandhu Sahoo ◽  
N. Jusna Devi ◽  
N. Ngashangva ◽  
P. Momota ◽  
Y. Rojeena ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Gc Content ◽  
Open Reading Frames ◽  
Draft Genome Sequence ◽  
Arthrobacter Globiformis ◽  
Content Type ◽  
A Genome ◽  
Reading Frames ◽  
Cave Deposit

Arthrobacter globiformis mrc11 was isolated from a Khangkhui cave deposit. Here, we report the draft genome sequence of this phylogenetically novel organism, which has a genome size of 4.89 Mb, a 65.9% GC content, and 4,657 predicted open reading frames that can be translated.

scholarly journals Complete Genome Sequence of Halomonas hydrothermalis Strain Slthf2, a Halophilic Bacterium Isolated from a Deep-Sea Hydrothermal-Vent Environment

2020 ◽  
Vol 9 (15) ◽  
Author(s):  
Naota Takeyama ◽  
Muyang Huang ◽  
Kensuke Sato ◽  
Josephine Galipon ◽  
Kazuharu Arakawa
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Deep Sea ◽  
Complete Genome ◽  
Gc Content ◽  
Halophilic Bacterium ◽  
Negative Bacterium ◽  
Content Type ◽  
South Pacific Ocean ◽  
A Genome

Halomonas hydrothermalis strain Slthf2 is a Gram-negative bacterium isolated from low-temperature hydrothermal fluids in South Pacific Ocean vent fields located at 2,580-m depth. Here, we report the complete genome sequence of this strain, which has a genome size of 4.12 Mb, with a GC content of 53.2%.

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