scholarly journals Effect of Octamethylcyclotetrasiloxane on Methylation of Bismuth by Methanosarcina barkeri

2006 ◽  
Vol 72 (10) ◽  
pp. 6819-6821 ◽  
Author(s):  
Klaus Michalke ◽  
Jörg Meyer ◽  
Reinhard Hensel

ABSTRACT Octamethylcyclotetrasiloxane (OMCTS), a common constituent of household products, triggers the transformation of bismuth to the volatile toxic derivative trimethylbismuth by Methanosarcina barkeri, which is a representative member of the sewage sludge microflora. Comparative studies with the ionophores monensin and lasalocid, which induce effects similar to those observed for OMCTS, indicated that the stimulation of bismuth methylation is not specific for the siloxane and suggested that the stimulation observed is mainly due to facilitated membrane permeation of the metal ion.


1987 ◽  
Vol 33 (6) ◽  
pp. 551-554 ◽  
Author(s):  
Ken F. Jarrell ◽  
Michelle Saulnier ◽  
Art Ley

The effect of ammonium chloride, sodium butyrate, sodium propionate, and the heavy metals nickel, zinc, and copper on methanogenesis by pure cultures of Methanospirillum hungatei, Methanosarcina barkeri, Methanobacterium thermoautotrophicum, and Methanobacterium formicicum at pH 6.5 was studied. The latter three strains were resistant to > 60 g/L of the volatile fatty acids and to > 10 g/L of NH3 N. Methanospirillum hungatei was somewhat more sensitive with 50% inhibition of methanogenesis occurring at 4.2 g/L NH3 N, 27 g/L butyrate, and 41 g/L propionate. All strains were very sensitive to both copper (1–5 mg/L) and zinc (1–10 mg/L), but much more resistant to nickel. Zinc and copper concentrations 30 to 270 times higher were required to cause inhibition of Msp. hungatei incubated in sewage sludge compared with buffer, indicating a strong protective environment was afforded the methanogens against heavy metal toxicity in the sludge.





Author(s):  
Yelizaveta Chernysh ◽  
Igor Roy ◽  
Viktoriia Chubur ◽  
Manabu Fukui ◽  
Ivan Koziy


2020 ◽  
Vol MA2020-02 (1) ◽  
pp. 114-114
Author(s):  
Alisha Yadav ◽  
Shu Nishijima ◽  
Takayuki Yamamoto ◽  
Toshiyuki Nohira




1991 ◽  
Vol 37 (2) ◽  
pp. 141-147 ◽  
Author(s):  
Lori K. Maas ◽  
Thomas L. Glass

Cellobiose transport by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes was measured using randomly tritiated cellobiose. When assayed at the same concentration (1 mM), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. The abilities of F. succinogenes to transport cellobiose or glucose were not affected by the sugar on which the cells were grown. Aspects of the simultaneous transport of [14C(U)]glucose and [3H(G)]cellobiose, the failure of high concentrations of cold glucose to compete with hypothetical [3H(G)] glucose (derived externally from [3H(G)]cellobiose), and differential metal-ion stimulation of cellobiose transport indicate a cellobiose permease, rather than cellobiase plus glucose permease, was responsible for cellobiose transport. Glucose (10-fold molar excess) partially inhibited cellobiose transport. This was enhanced by prior incubation of the cells with glucose, suggesting subsequent metabolism of the glucose was responsible for the inhibition. Compounds interfering with electron transport or maintenance of transmembrane ion gradients inhibited cellobiose uptake, indicating that active transport rather than a phosphoenolpyruvate:phosphotransferase system catalyzed cellobiose transport. Na+, but not Li+, stimulated cellobiose transport. Key words: Fibrobacter (Bacteroides) succinogenes, cellobiose transport, rumen bacteria.





2008 ◽  
Vol 42 (18) ◽  
pp. 4692-4698 ◽  
Author(s):  
Kenan Güney ◽  
Alexander Weidelener ◽  
Jörg Krampe


2000 ◽  
Vol 66 (7) ◽  
pp. 2791-2796 ◽  
Author(s):  
K. Michalke ◽  
E. B. Wickenheiser ◽  
M. Mehring ◽  
A. V. Hirner ◽  
R. Hensel

ABSTRACT Gases released from anaerobic wastewater treatment facilities contain considerable amounts of volatile methyl and hydride derivatives of metals and metalloids, such as arsine (AsH3), monomethylarsine, dimethylarsine, trimethylarsine, trimethylbismuth (TMBi), elemental mercury (Hg0), trimethylstibine, dimethyltellurium, and tetramethyltin. Most of these compounds could be shown to be produced by pure cultures of microorganisms which are representatives of the anaerobic sewage sludge microflora, i.e., methanogenic archaea (Methanobacterium formicicum,Methanosarcina barkeri, Methanobacterium thermoautotrophicum), sulfate-reducing bacteria (Desulfovibrio vulgaris, D. gigas), and a peptolytic bacterium (Clostridium collagenovorans). Additionally, dimethylselenium and dimethyldiselenium could be detected in the headspace of most of the pure cultures. This is the first report of the production of TMBi, stibine, monomethylstibine, and dimethylstibine by a pure culture of M. formicicum.



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