scholarly journals Longitudinal Analysis of Tick Densities and Borrelia, Anaplasma, and Ehrlichia Infections of Ixodes ricinus Ticks in Different Habitat Areas in The Netherlands

2006 ◽  
Vol 72 (12) ◽  
pp. 7594-7601 ◽  
Author(s):  
Peter R. Wielinga ◽  
Cor Gaasenbeek ◽  
Manoj Fonville ◽  
Albert de Boer ◽  
Ankje de Vries ◽  
...  

ABSTRACT From 2000 to 2004, ticks were collected by dragging a blanket in four habitat areas in The Netherlands: dunes, heather, forest, and a city park. Tick densities were calculated, and infection with Borrelia burgdorferi and Anaplasma and Ehrlichia species was investigated by reverse line blot analysis. The lowest tick density was observed in the heather area (1 to 8/100 m2). In the oak forest and city park, the tick densities ranged from 26 to 45/100 m2. The highest tick density was found in the dune area (139 to 551/100 m2). The infection rates varied significantly for the four study areas and years, ranging from 0.8 to 11. 5% for Borrelia spp. and 1 to 16% for Ehrlichia or Anaplasma (Ehrlichia/Anaplasma) spp. Borrelia infection rates were highest in the dunes, followed by the forest, the city park, and heather area. In contrast, Ehrlichia/Anaplasma was found most often in the forest and less often in the city park. The following Borrelia species were found: Borrelia sensu lato strains not identified to the species level (2.5%), B. afzelii (2.5%), B. valaisiana (0.9%), B. burgdorferi sensu stricto (0.13%), and B. garinii (0.13%). For Ehrlichia/Anaplasma species, Ehrlichia and Anaplasma spp. not identified to the species level (2.5%), Anaplasma schotti variant (3.5%), Anaplasma phagocytophilum variant (0.3%), and Ehrlichia canis (0.19%) were found. E. canis is reported for the first time in ticks in The Netherlands in this study. Borrelia lusitaniae, Ehrlichia chaffeensis, and the human granylocytic anaplasmosis agent were not detected. About 1.6% of the ticks were infected with both Borrelia and Ehrlichia/Anaplasma, which was higher than the frequency predicted from the individual infection rates, suggesting hosts with multiple infections or a possible selective advantage of coinfection.

2008 ◽  
Vol 74 (23) ◽  
pp. 7138-7144 ◽  
Author(s):  
Fedor Gassner ◽  
Patrick Verbaarschot ◽  
Renate C. Smallegange ◽  
Jeroen Spitzen ◽  
Sipke E. Van Wieren ◽  
...  

ABSTRACT The effect of introduced large herbivores on the abundance of Ixodes ricinus ticks and their Borrelia infections was studied in a natural woodland in The Netherlands. Oak and pine plots, either ungrazed or grazed by cattle, were selected. Ticks were collected weekly by blanket dragging. Borrelia infections were determined by PCR and restriction fragment length polymorphism. Rodent densities were estimated using mark-release-recapture methods. On occasion, the cattle were inspected for tick infestations. Meteorological data were recorded for each habitat. Significantly more ticks were collected in the ungrazed woodland than in the grazed woodland. The ungrazed oak habitat had higher tick densities than the pine habitat, while in the grazed habitats, tick densities were similar. Borrelia infection rates ranged from zero in larvae to 26% in nymphs to 33% in adult ticks, and B. afzelii, B. burgdorferi sensu stricto, B. garinii, and B. valaisiana were the species involved. Coinfections were found in five ticks. There was no effect of the presence of cattle on Borrelia infections in the ticks. In the ungrazed area, Borrelia infections in nymphs were significantly higher in the oak habitat than in the pine habitat. More mice were captured in the ungrazed area, and these had a significantly higher tick burden than mice from the grazed area. Tick burden on cattle was low. The results suggest that grazing has a negative effect on small rodents as well as on ticks but not on Borrelia infections. Implications of these results for management of woodland reserves and risk of Lyme disease are discussed.


2021 ◽  
Vol 9 (7) ◽  
pp. 1392
Author(s):  
Michiel Wijnveld ◽  
Anna-Margarita Schötta ◽  
Theresa Stelzer ◽  
Georg Duscher ◽  
Michael Leschnik ◽  
...  

We previously isolated and cultivated the novel Rickettsia raoultii strain Jongejan. This prompted us to ask whether this strain is unique or more widely present in Austria. To assess this issue, we retrospectively screened ticks collected from dogs in 2008. Of these collected ticks, we randomly selected 75 (47 females and 28 males) Dermacentor reticulatus, 44 (21 females, 7 males, and 16 nymphs) Haemaphysalis concinna, and 55 (52 females and 3 males) ticks of the Ixodes ricinus complex. Subsequently, these ticks were individually screened for the presence of tick-borne pathogens using the reverse line blot hybridization assay. In our current study, we detected DNA from the following microbes in D. reticulatus: Anaplasma phagocytophilum, Borrelia lusitaniae, Borrelia spielmanii, Borrelia valaisiana, and R. raoultii, all of which were R. raoultii strain Jongejan. In H. concinna, we found DNA of a Babesia sp., Rickettsia helvetica, and an organism closely related to Theileria capreoli. Lastly, I. ricinus was positive for Anaplasma phagocytophilum, Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii/Borrelia bavariensis, B. lusitaniae, B. spielmanii, B. valaisiana, Candidatus Neoehrlichia mikurensis, Rickettsia helvetica, Rickettsia monacensis, and Theileria (Babesia) microti DNA. The detection of DNA of the Babesia sp. and an organism closely related to Theileria capreoli, both found in H. concinna ticks, is novel for Austria.


2021 ◽  
Vol 7 (1) ◽  
pp. 54
Author(s):  
Eduardo Bagagli ◽  
Daniel Ricardo Matute ◽  
Hans Garcia Garces ◽  
Bernardo Guerra Tenório ◽  
Adalberto Garcia Garces ◽  
...  

Paracoccidioidomycosis is an endemic fungal disease to Latin America caused by at least five species-level genotypes of Paracoccidioides, named P. lutzii, P. brasiliensis (S1a and S1b populations), P. americana, P. restrepiensis, and P. venezuelensis. In this manuscript, we report on Paracoccidioides sp. sampling efforts in armadillos from two different areas in Brazil. We sequenced the genomes of seven Paracoccidioides isolates and used phylogenomics and populations genetics for genotyping. We found that P. brasiliensis and P. lutzii are both present in the Amazon region. Additionally, we identified two Paracoccidioides isolates that seem to be the result of admixture between divergent populations within P. brasiliensis sensu stricto. Both of these isolates were recovered from armadillos in a P. lutzii endemic area in Midwestern Brazil. Additionally, two isolates from human patients also show evidence of resulting from admixture. Our results suggest that the populations of P. brasiliensis sensu stricto exchange genes in nature. More generally, they suggest that population structure and admixture within species is an important source of variation for pathogenic fungi.


1921 ◽  
Vol 15 (4) ◽  
pp. 503-510
Author(s):  
James Brown Scott

A conference of a group of Powers heretofore known as the Principal Allied and Associated Powers (the British Empire, France, Italy, Japan and the United States), to discuss the limitation of armament, and of these Powers, and Belgium, China, the Netherlands and Portugal, to consider Pacific and Far Eastern problems, will open in the City of Washington on November 11, 1921.


2021 ◽  
Author(s):  
Michel Schreinemachers ◽  
Wiebe Strick

<p>Should a bridge always be functional and accessible? Should it always fulfil its purpose? This seemingly self- evident question is a key question in footbridge design that is oriented towards creating experiences.</p><p>Footbridges are able to successfully enriches our experience of a certain context or landscape, it cannot be functional all the time, under all environmental conditions, weather and seasons. A good example is the Zalige bridge designed as part of the Room for the River, a large-scale national program for inland flood- protection in the Netherlands. Build upon the floodplains within a newly created river-park by the city of Nijmegen, the Zalige bridge’s curved shape stands in direct relationship to the fluctuating water levels of the river. When water levels rise, the bridge partially submerges, becoming only accessible through steppingstones. At peak heights, the bridge disappears completely, becoming a metaphor for our relationship to the water.</p><p>“Building a bridge that fails to fulfil its sole purpose of containing the water; this can only be pulled off in the Netherlands.” – jury Dutch Design Awards about the Zalige bridge.</p><p>The loss of functionality is directly related to the creation of an experience. When the water levels rose in January 2018, the bridge became the prime location to experience the changing landscape. It shows that engineering a bridge is not solely focussed on the most efficient engineering, but for the purpose it fulfils as for society. For most pedestrian bridges where the perception of the user is on a different level as for a highway bridge, functionality provides more than just cost driven or efficiency driven parameters. It is more related to the added value for the community. When design not solemnly derives from the sheer taste and predilection of the designer but is based on the user’s experience, it generates a durable relation with a feeling of ownership of its users. The key is to create this experience in an elegant and natural way and not forced or dictated. It should be people's own unique discovery and should not be imposed.</p>


2021 ◽  
pp. 133-140
Author(s):  
L. I. ATKINA ◽  
◽  
L. V. BULATOVA ◽  
L. P. ABRAMOVA

Based on a comprehensive assessment of the state of the park landscape, the level of its anthropogenic transformation is determined, which is necessary to identify the potential for restoring the natural biodiversity of the object. It is reported that the plantations and soil cover of the park of the 50th anniversary of VLKSM are very heterogeneous; they reflect the history of park establishment. The soil cover consists of three main types: sod, bog and urbanozem. It should be stressed that the best preserved part of the park is the plot with boggy soils around the pond. Herbaceous plants growing on the plot are very similar to the species composition of the ground cover of overgrown peat bogs. Over the 40 years since the foundation of the park, a high level of pollutants has been accumulated in the soil, which allows us to state that the negative impact occurs constantly. Consequently, the existing plantings are insufficient to protect visitors from vehicle emissions from the roads along Yasnaya and Shaumyana streets. There are enough natural elements in the park of the 50th anniversary of VLKSM; this should be taken into account in its redevelopment. There is an opportunity to restore the reservoir and return the plantings to their natural appearance. It is proposed to add elements of landscape decoration in the park.


2002 ◽  
Vol 37 (0) ◽  
pp. 913-913
Author(s):  
Masahito Shimizu ◽  
Naoji Matsumoto
Keyword(s):  
The City ◽  

2020 ◽  
pp. 300-308
Author(s):  
Jonathan Scott

This concluding chapter discusses how the Anglo-Dutch revolution of 1649–1702 stood at the centre of a succession of wider transformations which were agricultural, political, and commercial. All of these had their origins in the Netherlands before spreading to south-eastern England and across the Atlantic. Understanding their development and diffusion has required attention to religion, migration, and war as well as to economic, social, and cultural life. The result connected a series of unique local human environments, including the Dutch water world, the city of London, and the American frontier into a world-altering imperial system. By the later eighteenth century the Atlantic reorientation of the European economy had thrown the Baltic into relative decline, sparking the dramatic growth of Liverpool, Manchester, and Glasgow while Stockholm, Copenhagen, and Amsterdam stagnated.


1999 ◽  
Vol 73 (2) ◽  
pp. 95-102 ◽  
Author(s):  
B.S. Dezfuli ◽  
E. Rossetti ◽  
C.M. Bellettato ◽  
B.J. Maynard

Infection rates of larvalPomphorhynchus laevisMu¨ller, 1776 (Acanthocephala) were studied in its intermediate hostEchinogammarus stammeriS. Karaman, 1931 (Amphipoda). Crustaceans were collected monthly, from July 1990 to July 1997, at two sites on the River Brenta (northern Italy). Of over 50,000E. stammeriexamined, more than 24% were parasitized withP. laevislarvae. The differences in the prevalence of infection between the two stations were significant. The intensity of infection varied from one to 15 acanthocephalans per host. Amphipods with multiple infections constituted 28–32% of each monthly sample. During the 7 years of investigation, the prevalence of infection typically reached its highest and lowest levels in the summer and spring months, respectively. Acanthocephalan larvae showed no preference for host sex. Infected ovigerous females ofE. stammeriwere found carrying eggs or juveniles in their brood pouches, but had fewer and smaller eggs.Pomphorhynchus laevislarvae co-occurred withAcanthocephalus clavula(Dujardin, 1845) and also with the tapewormCyathocephalus truncatus(Pallas, 1781) in the same host.


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