scholarly journals The Salmonella Pathogenicity Island 2-Encoded Type III Secretion System Is Essential for the Survival of Salmonella enterica Serovar Typhimurium in Free-Living Amoebae

2009 ◽  
Vol 75 (6) ◽  
pp. 1793-1795 ◽  
Author(s):  
Benjamin Bleasdale ◽  
Penelope J. Lott ◽  
Aparna Jagannathan ◽  
Mark P. Stevens ◽  
Richard J. Birtles ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Type Iii Secretion System ◽  
Pathogenicity Island ◽  
Secretion System ◽  
Free Living ◽  
Type Iii ◽  
Serovar Typhimurium ◽  
Potential Reservoir

ABSTRACT Free-living amoebae represent a potential reservoir and predator of Salmonella enterica. Through the use of type III secretion system (T3SS) mutants and analysis of transcription of selected T3SS genes, we demonstrated that the Salmonella pathogenicity island 2 is highly induced during S. enterica serovar Typhimurium infection of Acanthamoeba polyphaga and is essential for survival within amoebae.

Safety and immunogenicity of attenuated Salmonella enterica serovar Typhimurium delivering an HIV-1 Gag antigen via the Salmonella Type III secretion system

Vaccine ◽  
2006 ◽  
Vol 24 (37-39) ◽  
pp. 6216-6224 ◽  
Author(s):  
Camille N. Kotton ◽  
Alexander J. Lankowski ◽  
Nathaniel Scott ◽  
David Sisul ◽  
Li Mei Chen ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
Serovar Typhimurium ◽  
Hiv 1

scholarly journals SigE Is a Chaperone for the Salmonella enterica Serovar Typhimurium Invasion Protein SigD

2001 ◽  
Vol 183 (4) ◽  
pp. 1452-1454 ◽  
Author(s):  
K. Heran Darwin ◽  
Lloyd S. Robinson ◽  
Virginia L. Miller
Keyword(s):  
Hybrid System ◽  
Type Iii Secretion ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
Serovar Typhimurium ◽  
Two Hybrid

ABSTRACT SigD is translocated into eucaryotic cells by a type III secretion system. In this work, evidence that the putative chaperone SigE directly interacts with SigD is presented. A bacterial two-hybrid system demonstrated that SigE can interact with itself and SigD. In addition, SigD was specifically copurified with SigE-His6on a nickel column.

scholarly journals Secretion of the orgC Gene Product by Salmonella enterica Serovar Typhimurium

2003 ◽  
Vol 71 (11) ◽  
pp. 6680-6685 ◽  
Author(s):  
James B. Day ◽  
Catherine A. Lee
Keyword(s):  
Gene Product ◽  
Type Iii Secretion ◽  
Salmonella Enterica ◽  
Virulence Genes ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Type Iii Secretion System ◽  
Pathogenicity Island ◽  
Secretion System ◽  
Serovar Typhimurium ◽  
Transposon Insertions

ABSTRACT HilA activates the transcription of genes on Salmonella pathogenicity island 1 (SPI1), which encodes a type III secretion system (TTSS). Previous studies showed that transposon insertions in orgC, a gene located on SPI1, increase hilA expression. We characterize the orgC gene product and show that it is secreted via the SPI1 TTSS. We propose a model whereby OrgC functions as a secreted repressor of the SPI1 virulence genes.

scholarly journals In Vivo Genetic Analysis Indicates That PhoP-PhoQ and the Salmonella Pathogenicity Island 2 Type III Secretion System Contribute Independently to Salmonella enterica Serovar Typhimurium Virulence

2001 ◽  
Vol 69 (12) ◽  
pp. 7254-7261 ◽  
Author(s):  
Carmen R. Beuzón ◽  
Kate E. Unsworth ◽  
David W. Holden
Keyword(s):  
Virulence Factors ◽  
Type Iii Secretion ◽  
Salmonella Enterica ◽  
Type Iii Secretion System ◽  
Pathogenicity Island ◽  
Secretion System ◽  
Type Iii ◽  
Serovar Typhimurium

ABSTRACT Many virulence factors are required for Salmonella enterica serovar Typhimurium to replicate intracellularly and proliferate systemically within mice. In this work, we have carried out genetic analyses in vivo to determine the functional relationship between two major virulence factors necessary for systemic infection byS. enterica serovar Typhimurium: theSalmonella pathogenicity island 2 (SPI-2) type III secretion system (TTSS) and the PhoP-PhoQ two-component regulatory system. Although previous work suggested that PhoP-PhoQ regulates SPI-2 TTSS gene expression in vitro, in vivo competitive analysis of mutant strains indicates that these systems contribute independently toS. typhimurium virulence. Our results also suggest that mutation of phoP may compensate partially for defects in the SPI-2 TTSS by deregulating SPI-1 TTSS expression. These results provide an explanation for previous reports showing an apparent functional overlap between these two systems in vitro.

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