scholarly journals Detection of Enterobacter sakazakii in Dried Infant Milk Formula by Cationic-Magnetic-Bead Capture

2006 ◽  
Vol 72 (9) ◽  
pp. 6325-6330 ◽  
Author(s):  
N. R. Mullane ◽  
J. Murray ◽  
D. Drudy ◽  
N. Prentice ◽  
P. Whyte ◽  
...  
Keyword(s):  
Birth Weight ◽  
Food Chain ◽  
Magnetic Bead ◽  
Milk Formula ◽  
Enterobacter Sakazakii ◽  
Low Birth Weight Infants ◽  
Simple Method ◽  
Infant Milk Formula ◽  
Selective Agar ◽  
Life Threatening

ABSTRACT Enterobacter sakazakii has been associated with life-threatening infections in premature low-birth-weight infants. Contaminated infant milk formula (IMF) has been implicated in cases of E. sakazakii meningitis. Quick and sensitive methods to detect low-level contamination sporadically present in IMF preparations would positively contribute towards risk reduction across the infant formula food chain. Here we report on the development of a simple method, combining charged separation and growth on selective agar, to detect E. sakazakii in IMF. This protocol can reliably detect 1 to 5 CFU of E. sakazakii in 500 g of IMF in less than 24 h.

scholarly journals Fish Oil Monotherapy for Intestinal Failure-Associated Liver Disease on SMOFlipid in the Neonatal Intensive Care Unit

2020 ◽  
Vol 9 (11) ◽  
pp. 3393
Author(s):  
Sanghoon Lee ◽  
Se In Sung ◽  
Hyo Jung Park ◽  
Yun Sil Chang ◽  
Won Soon Park ◽  
...  
Keyword(s):  
Liver Disease ◽  
Birth Weight ◽  
Fish Oil ◽  
Neonatal Intensive Care ◽  
Lipid Emulsion ◽  
Intestinal Failure ◽  
Direct Bilirubin ◽  
Low Birth Weight Infants ◽  
Life Threatening

Intestinal failure-associated liver disease (IFALD) is a life-threatening complication of parenteral nutrition (PN) and is most prevalent in the preterm neonatal population receiving long-term PN. In this study, we report the outcome of our experience with fish oil monotherapy for IFALD in a fish oil-based combination lipid emulsion administered to preterm low birth weight infants. Fasting neonates were administered as PN according to our center’s nutrition protocol. A diagnosis of IFALD was made when the serum direct bilirubin levels were >2.0 mg/dL in two consecutive measurements that were more than one week apart, without evidence of intrinsic causes of liver dysfunction. The management of IFALD was conducted by switching the lipid emulsion from combination lipid emulsion to fish oil monotherapy at 1.0 g/kg/day, infused over 24 h. Fifteen infants met the criteria for IFALD and received fish oil monotherapy. The median gestational age was 27.5 weeks and the median birth weight was 862.5 g. IFALD was successfully reversed in 11 infants (11/15, 73.3%). The median duration of fish oil monotherapy was 39 days. Direct bilirubin values were initially elevated and then steadily declined from the third week of treatment onward. The enteral tolerance increased in varying degrees during the treatment period. The mean weight gain was 26.0 g/day during fish oil monotherapy. Omegaven® (Fresenius Kabi Austria Gmbh, Graz, Austria) at a dose of 1.0 g/kg/day was well tolerated, and no adverse events related to Omegaven use were seen. The reversal of IFALD in preterm infants on combination lipid emulsion containing fish oil was achieved by switching to fish oil monotherapy.

scholarly journals Detergent and Sanitizer Stresses Decrease the Thermal Resistance of Enterobacter sakazakii in Infant Milk Formula

2008 ◽  
Vol 73 (3) ◽  
pp. M154-M157 ◽  
Author(s):  
T.M. Osaili ◽  
R.R. Shaker ◽  
A.N. Olaimat ◽  
A.A. Al-Nabulsi ◽  
M.A. Al-Holy ◽  
...  
Keyword(s):  
Thermal Resistance ◽  
Milk Formula ◽  
Enterobacter Sakazakii ◽  
Infant Milk Formula

"LATE EDEMA" IN LOW BIRTH WEIGHT INFANTS

PEDIATRICS ◽  
1968 ◽  
Vol 41 (1) ◽  
pp. 67-76
Author(s):  
Paul Y. K. Wu ◽  
William Oh ◽  
Alberto Lubetkin ◽  
Jack Metcoff ◽  
Marjorie Gabler ◽  
...  
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Milk Intake ◽  
Milk Formula ◽  
Extracellular Water ◽  
Low Birth Weight Infants ◽  
Sodium Potassium ◽  
Internal Distribution ◽  
Sodium And Potassium

Twenty 48-hour balance studies were performed on six low birth weight infants with late edema and on four control infants. Each infant had two balance studies: one on a regular milk formula and another on a lower sodium and potassium milk preparation. The urinary outgo of sodium. potassium, nitrogen, and water was similar in both groups of infants, irrespective of the type of milk intake. The internal distribution of electrolyte and water was calculated from the balance data. In the control infants, the retained water and sodium was distributed between the intracellular and extracellular phases. In infants with late edema, the expansion of the extracellular water and sodium was equivalent to the sum of the net retention, plus an internal shift of water and sodium from the non-extracellular or intracellular phases into the extracellular phase.

On the risk of Enterobacter sakazakii in infant milk formula

2004 ◽  
Vol 15 (2) ◽  
pp. 99-100 ◽  
Author(s):  
Arie H. Havelaar ◽  
Marcel Zwietering
Keyword(s):  
Milk Formula ◽  
Enterobacter Sakazakii ◽  
Infant Milk Formula

Effects of Extended Dry Storage of Powdered Infant Milk Formula on Susceptibility of Enterobacter sakazakii to Hot Water and Ionizing Radiation

2008 ◽  
Vol 71 (5) ◽  
pp. 934-939 ◽  
Author(s):  
TAREQ M. OSAILI ◽  
ANAS A. AL-NABULSI ◽  
REYAD R. SHAKER ◽  
MUTAMED M. AYYASH ◽  
AMIN N. OLAIMAT ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Gamma Radiation ◽  
Low Dose ◽  
Hot Water ◽  
Neonatal Meningitis ◽  
Milk Formula ◽  
Enterobacter Sakazakii ◽  
Dry Storage ◽  
Potential Source ◽  
Infant Milk Formula

Infant milk formula has been identified as a potential source of Enterobacter sakazakii, which has been implicated in neonatal meningitis and necrotizing enterocolitis. This study was undertaken to determine whether the length of E. sakazakii storage in powdered infant milk formula (PIMF) affected the ability of the pathogen to survive subsequent reconstitution of the powder with hot water or treatment with gamma radiation. Five E. sakazakii strains were mixed individually with PIMF and kept for up to 12 months at 25°C. After storage PIMF was reconstituted with water at 60 to 100°C or was exposed to ≤5 kGy of gamma radiation. Without any treatment secondary to drying, E. sakazakii counts decreased <1 log/g after 1 month but decreased about 4 log/g during storage for 8 to 12 months. Dry storage decreased thermal resistance but increased resistance of E. sakazakii to ionizing radiation in PIMF. Reconstitution of contaminated powder with water at 70°C after 1 month of dry storage reduced E. sakazakii viability slightly, >2 log/g, and after powder was stored for 12 months all E. sakazakii strains were eliminated. In contrast, desiccation substantially increased the resistance of E. sakazakii strains to ionizing radiation. Although the D-value for E. sakazakii IMF1 following overnight storage in PIMF was 0.98 kGy, >4 kGy was required to kill 1.5 log/g of the same strain that had survived 12 months in dry PIMF. Results suggested that low-dose irradiation will more effectively eliminate E. sakazakii from PIMF if the treatment is applied shortly after PIMF manufacture.

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