Improved Secretion of Native Human Insulin-Like Growth Factor 1 from gas1 Mutant Saccharomyces cerevisiae Cells
2000 ◽
Vol 66
(12)
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pp. 5477-5479
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Keyword(s):
Factor Α
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ABSTRACT We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1gene was fused to the mating factor α prepro- leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.
1991 ◽
Vol 198
(3)
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pp. 651-657
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1992 ◽
Vol 267
(18)
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pp. 12692-12699
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Keyword(s):
2000 ◽
Vol 85
(10)
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pp. 3604-3608
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1991 ◽
Vol 9
(4)
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pp. 503-515
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