scholarly journals Identification and Characterization of Pathogenic Aeromonas veronii Biovar Sobria Associated with Epizootic Ulcerative Syndrome in Fish in Bangladesh

2002 ◽  
Vol 68 (2) ◽  
pp. 650-655 ◽  
Author(s):  
Mokhlasur Rahman ◽  
Patricia Colque-Navarro ◽  
Inger K�hn ◽  
Geert Huys ◽  
Jean Swings ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Acid Methyl Ester ◽  
Fish Cell ◽  
Environmental Isolates ◽  
Fish Cell Lines ◽  
Aeromonas Veronii ◽  
Diseased Fish ◽  
Epizootic Ulcerative Syndrome ◽  
Identification And Characterization

ABSTRACT Sparse information is available on the virulence factors of Aeromonas strains isolated from diseased fish, from the environment, and from humans. In the present study, 52 Aeromonas isolates obtained from epizootic ulcerative syndrome (EUS) lesions in fish, from the aquatic environment, and from children with diarrhea in Bangladesh were identified by biochemical phenotyping (i.e., PhenePlate [PhP] typing) and DNA fingerprinting and then characterized with respect to certain putative virulence factors. The isolates from the fish exhibiting EUS symptoms were identified to be Aeromonas veronii biovar sobria by fatty acid methyl ester analysis and amplified fragment length polymorphism fingerprinting. Biochemical phenotyping revealed that all EUS-associated isolates belonged to a unique phenotype which was not identified among more than 1,600 environmental and diarrheal isolates in a previously collected database of PhP types of Bangladeshi Aeromonas isolates. The 52 Aeromonas isolates were investigated for the production of hemolysin and cytotoxin; for hemagglutination with erythrocytes from fish, human, and rabbit sources; for the presence of a cytolytic enterotoxin gene; and for adhesion to and invasion into fish cell lines. All of the EUS isolates produced all of the virulence factors investigated, as did also some of the environmental isolates, but the isolates from EUS were unique in their ability to agglutinate fish erythrocytes. Our results suggest that a clonal group of A. veronii biovar sobria is associated with, and may be a causative agent of, EUS in fish in Bangladesh.

Fish cell lines: Establishment and characterization of nine cell lines from salmonids

In Vitro ◽  
1984 ◽  
Vol 20 (9) ◽  
pp. 671-676 ◽  
Author(s):  
C. N. Lannan ◽  
J. R. Winton ◽  
J. L. Fryer
Keyword(s):  
Cell Lines ◽  
Fish Cell ◽  
Fish Cell Lines

scholarly journals Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection

2016 ◽  
Vol 17 (2) ◽  
pp. 154 ◽  
Author(s):  
Xiaodan Liu ◽  
Jiagang Tu ◽  
Junfa Yuan ◽  
Xueqin Liu ◽  
Lijuan Zhao ◽  
...  
Keyword(s):  
Cell Line ◽  
Fish Cell Line ◽  
Fish Cell ◽  
Identification And Characterization

scholarly journals Molecular and Cytogenetic Characterization of Fish Cell Lines and its Application in Aquatic Research

2015 ◽  
Vol 39 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Naresh Sahebrao Nagpure ◽  
Akhilesh Kr. Mishra ◽  
A. S. Ninawe ◽  
Avinash Rasal ◽  
Akhilesh Dubey ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Cytogenetic Characterization

Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines

2002 ◽  
Vol 20 (3) ◽  
pp. 226-231
Author(s):  
Guo Hua-rong ◽  
Zhang Shi-cui ◽  
Li Hong-yan ◽  
Tong Shang-liang ◽  
Xiang Jian-hai
Keyword(s):  
Lactate Dehydrogenase ◽  
Cell Lines ◽  
Marine Fish ◽  
Isozyme Pattern ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Dehydrogenase Isozyme ◽  
Lactate Dehydrogenase Isozyme

scholarly journals Identification and characterization of two transmembrane proteins required for virulence of Ustilago maydis

2021 ◽  
Author(s):  
Paul Weiland ◽  
Florian Altegoer
Keyword(s):  
Virulence Factors ◽  
Biochemical Analysis ◽  
Transmembrane Proteins ◽  
Ustilago Maydis ◽  
Effector Proteins ◽  
Smut Fungi ◽  
Initial Characterization ◽  
Associated Proteins ◽  
Identification And Characterization

AbstractSmut fungi comprise a large group of biotrophic phytopathogens infecting important crops such as wheat and corn. Through the secretion of effector proteins, the fungus actively suppresses plant immune reactions and modulates its host’s metabolism. Consequently, how soluble effector proteins contribute to virulence is already characterized in a range of phytopathogens. However, membrane-associated virulence factors have been much less studied to date. Here, we investigated six transmembrane (TM) proteins that show elevated gene expression during biotrophic development of the maize pathogen Ustilago maydis. We show that two of the six proteins, named Vmp1 and Vmp2 (virulence-associated membrane protein), are essential for the full virulence of U. maydis. The deletion of the corresponding genes lead to a substantial attenuation in the virulence of U. maydis. Furthermore, both are conserved in various related smuts and contain no domains of known function. Our biochemical analysis clearly shows that Vmp1 and Vmp2 are membrane-associated proteins, potentially localizing to the U. maydis plasma membrane. Mass photometry and light scattering suggest that Vmp1 mainly occurs as a monomer, while Vmp2 is dimeric. Notably, the large and partially unstructured C-terminal domain of Vmp2 is crucial for virulence while not contributing to dimerization. Taken together, we here provide an initial characterization of two membrane proteins as virulence factors of U. maydis.

scholarly journals Identification and Characterization of Two Transmembrane Proteins Required for Virulence of Ustilago maydis

2021 ◽  
Vol 12 ◽  
Author(s):  
Paul Weiland ◽  
Florian Altegoer
Keyword(s):  
Virulence Factors ◽  
Biochemical Analysis ◽  
Transmembrane Proteins ◽  
Ustilago Maydis ◽  
Effector Proteins ◽  
Smut Fungi ◽  
Initial Characterization ◽  
Associated Proteins ◽  
Identification And Characterization

Smut fungi comprise a large group of biotrophic phytopathogens infecting important crops such as wheat and corn. Through the secretion of effector proteins, the fungus actively suppresses plant immune reactions and modulates its host’s metabolism. Consequently, how soluble effector proteins contribute to virulence is already characterized in a range of phytopathogens. However, membrane-associated virulence factors have been much less studied to date. Here, we investigated six transmembrane (TM) proteins that show elevated gene expression during biotrophic development of the maize pathogen Ustilago maydis. We show that two of the six proteins, named Vmp1 and Vmp2 (virulence-associated membrane protein), are essential for the full virulence of U. maydis. The deletion of the corresponding genes leads to a substantial attenuation in the virulence of U. maydis. Furthermore, both are conserved in various related smuts and contain no domains of known function. Our biochemical analysis clearly shows that Vmp1 and Vmp2 are membrane-associated proteins, potentially localizing to the U. maydis plasma membrane. Mass photometry and light scattering suggest that Vmp1 mainly occurs as a monomer, while Vmp2 is dimeric. Notably, the large and partially unstructured C-terminal domain of Vmp2 is crucial for virulence while not contributing to dimerization. Taken together, we here provide an initial characterization of two membrane proteins as virulence factors of U. maydis.

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