Characterization of Erwinia amylovora Strains from Bulgaria by Pulsed-Field Gel Electrophoresis

2012 ◽  
Vol 67 ◽  
pp. 0187 ◽  
Author(s):  
Iliana Atanasova ◽  
Zoltan Urshev ◽  
Petya Hristova ◽  
Nevena Bogatzevska ◽  
Penka Moncheva
Keyword(s):  
Gel Electrophoresis ◽  
Erwinia Amylovora ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Molecular Characterization of Natural Erwinia pyrifoliae Strains Deficient in Hypersensitive Response

2003 ◽  
Vol 69 (1) ◽  
pp. 679-682 ◽  
Author(s):  
Susanne Jock ◽  
Won-Sik Kim ◽  
Marie-Anne Barny ◽  
Klaus Geider
Keyword(s):  
Molecular Characterization ◽  
Hypersensitive Response ◽  
Gel Electrophoresis ◽  
Erwinia Amylovora ◽  
Pulsed Field Gel Electrophoresis ◽  
Necrotic Tissue ◽  
Tobacco Leaves ◽  
Pulsed Field ◽  
Pear Tree

ABSTRACT From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with intact genes from E. pyrifoliae and Erwinia amylovora.

Characterization of Erwinia amylovora Strains from Bulgaria by Pulsed-Field Gel Electrophoresis

2012 ◽  
Vol 67 (3-4) ◽  
pp. 187-194 ◽  
Author(s):  
Iliana Atanasova ◽  
Zoltan Urshev ◽  
Petya Hristova ◽  
Nevena Bogatzevska ◽  
Penka Moncheva
Keyword(s):  
Genetic Differentiation ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Erwinia Amylovora ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Profile ◽  
Pfge Analysis ◽  
Pulsed Field ◽  
Pfge Profiles

The aim of this study was to characterize genetically Bulgarian Erwinia amylovora strains using pulsed-field gel electrophoresis (PFGE) analysis. Fifty E. amylovora strains isolated from different hosts, locations, as well as in different years were analysed by PFGE after XbaI, SpeI, and XhoI digestion of the genomic DNA. The strains were distributed into four groups according to their XbaI-generated profile. About 82% of the strains displayed a PFGE profile identical to that of type Pt2. Three strains belonged to the Central Europe Pt1 type. Two new PFGE profi les, not reported so far, were established - one for a strain isolated from Malus domestica and another for all Fragaria spp. strains. The same grouping of the strains was obtained after analysis of the SpeI digestion patterns. On the basis of PFGE profiles, after XbaI and SpeI digestion, a genetic differentiation between the strains associated with subfamily Maloideae and subfamily Rosoideae was revealed. The presence of more than one PFGE profi le in the population of E. amylovora in Bulgaria suggests a multiple source of inoculum

scholarly journals Characterization of Staphylococcus intermedius from Pigeons, Dogs, Foxes, Mink, and Horses by Pulsed-Field Gel Electrophoresis

2002 ◽  
Vol 64 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Yoshihisa WAKITA ◽  
Akira SHIMIZU ◽  
Václav HÁJEK ◽  
Junichi KAWANO ◽  
Kenji YAMASHITA
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Staphylococcus Intermedius

scholarly journals Application of pulsed-field gel electrophoresis to the epidemiological characterization ofStaphylococcus intermediusimplicated in a food-related outbreak

1994 ◽  
Vol 113 (1) ◽  
pp. 75-81 ◽  
Author(s):  
F. M. Khambaty ◽  
R. W. Bennett ◽  
D. B. Shah
Keyword(s):  
Gel Electrophoresis ◽  
Pfge Pattern ◽  
Pulsed Field Gel Electrophoresis ◽  
Western United States ◽  
Single Strain ◽  
Pulsed Field ◽  
Definitive Evidence ◽  
Staphylococcus Intermedius ◽  
Epidemiological Characterization

SUMMARYAn outbreak of food intoxication involving over 265 cases in western United States occurred in October 1991.Staphylococcus intermediuswas implicated as the aetiologic agent. Representative outbreak isolates (five clinical and ten from foods) produced type A enterotoxin. DNA fragments generated by four restriction endonucleases and analysed by pulsed-field gel electrophoresis (PFGE) provided definitive evidence that all isolates from nine different counties in California and Nevada were derived from a single strain. The PFGE pattern of these outbreak isolates was distinct from those of a heterogeneous collection of sevenS. intermediusstrains of veterinary origin and five unrelatedS. aureuslaboratory strains. The data show a significant PFGE pattern heterogeneity not only among members of differentStaphylococcusspecies but also within members of the same species and even the same enterotoxin type. The results indicate that PFGE is a valuable strain-specific discriminator for the epidemiological characterization ofS. intermedius. To our knowledge, this represents the first documented foodborne outbreak caused byS. intermedius. These findings suggest that the presence ofS. intermediusand other species such asS. hyicusin food should be reason for concern.

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