scholarly journals Description of Treponema azotonutricium sp. nov. and Treponema primitia sp. nov., the First Spirochetes Isolated from Termite Guts

2004 ◽  
Vol 70 (3) ◽  
pp. 1315-1320 ◽  
Author(s):  
Joseph R. Graber ◽  
Jared R. Leadbetter ◽  
John A. Breznak
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Complete Characterization ◽  
Free Living ◽  
16S Rrna Sequence ◽  
Gene Encoding ◽  
Phenotypic Properties ◽  
First Time ◽  
Specific Epithets

ABSTRACT Long after their original discovery, termite gut spirochetes were recently isolated in pure culture for the first time. They revealed metabolic capabilities hitherto unknown in the Spirochaetes division of the Bacteria, i.e., H2 plus CO2 acetogenesis (J. R. Leadbetter, T. M. Schmidt, J. R. Graber, and J. A. Breznak, Science 283:686-689, 1999) and dinitrogen fixation (T. G. Lilburn, K. S. Kim, N. E. Ostrom, K. R. Byzek, J. R. Leadbetter, and J. A. Breznak, Science 292:2495-2498, 2001). However, application of specific epithets to the strains isolated (Treponema strains ZAS-1, ZAS-2, and ZAS-9) was postponed pending a more complete characterization of their phenotypic properties. Here we describe the major properties of strain ZAS-9, which is readily distinguished from strains ZAS-1 and ZAS-2 by its shorter mean cell wavelength or body pitch (1.1 versus 2.3 μm), by its nonhomoacetogenic fermentation of carbohydrates to acetate, ethanol, H2, and CO2, and by 7 to 8% dissimilarity between its 16S rRNA sequence and those of ZAS-1 and ZAS-2. Strain ZAS-9 is proposed as the type strain of the new species, Treponema azotonutricium. Strains ZAS-1 and ZAS-2, which are H2-consuming, CO2-reducing homoacetogens, are proposed here to be two strains of the new species Treponema primitia. Apart from the salient differences mentioned above, the genomes of all three strains were similar in size (3,461 to 3,901 kb), in G+C content (50.0 to 51.0 mol%), and in possession of 2 copies of the gene encoding 16S rRNA (rrs). For comparison, the genome of the free-living spirochete Spirochaeta aurantia strain J1 was analyzed by the same methods and found to have a size of 3,719 kb, to contain 65.6 mol% G+C, and also to possess 2 copies of the rrs gene.

scholarly journals Characterization Physiology and Molecular Bacteria Symbiont- Entomopathogenic Nematodes based of Gene Sequences Encoding the 16S rRNA District of Bromo Probolinggo

2017 ◽  
Vol 18 (1) ◽  
pp. 39
Author(s):  
Bagus Setiawan ◽  
Didik Sulistyanto ◽  
Kartika Senjarini
Keyword(s):  
16S Rrna ◽  
Entomopathogenic Nematodes ◽  
Phenotypic Characterization ◽  
Bacterial Symbionts ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Gene Encoding ◽  
Bacillus Strain ◽  
Rrna Sequences

This study aims to identify entomopathogenic nematodes symbiotic bacteria phenotypically and based on the gene encoding 16S rRNA sequences. Bacterial symbionts of entomopathogenic nematodes, obtained from isolates from the area Wonokerto (WN01) and isolates Sukapura (SP01), Bromo, Probolinggo, two symbiont bacteria was found in entomopathogenic nematodes Steinernema sp. The method used in this study are: the isolation of entomopathogenic nematodes Steinernema sp. and bacterial symbionts conventionally for the identification of phenotypically, after the characterization of bacterial isolates, the isolation of genomic DNA, 16S rRNA PCR, DNA purification and DNA sequence analysis. The results based on phenotypic characterization showed that isolates WN01 and SP01, yellowish white, gram positive, negative bioluminenscene, catalase positive, can not hydrolyze urea, and also can not produce H2S. The results of the gene encoding 16S rRNA sequence can be deduced WN01 isolates have in common with the bacteria Bacillus strain toyonensis BCT 7112, while the SP01 isolates have in common with the bacteria Bacillus strain cereus ATCC 14 579.

scholarly journals Molecular Characterization of the Gene Encoding a New AmpC β-Lactamase in a Clinical Strain of Acinetobacter Genomic Species 3

2004 ◽  
Vol 48 (4) ◽  
pp. 1374-1378 ◽  
Author(s):  
Alejandro Beceiro ◽  
Lourdes Dominguez ◽  
Anna Ribera ◽  
Jordi Vila ◽  
Francisca Molina ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Characterization ◽  
Biochemical Properties ◽  
Clinical Strain ◽  
The Novel ◽  
Gene Encoding ◽  
Genomic Species ◽  
First Time

ABSTRACT A presumptive chromosomal cephalosporinase (pI, 9.0) from a clinical strain of Acinetobacter genomic species 3 (AG3) is reported. The nucleotide sequence of this β-lactamase shows for the first time the gene encoding an AmpC enzyme in AG3. In addition, the biochemical properties of the novel AG3 AmpC β-lactamase are reported

A new sand frog from Namaqualand, South Africa (Pyxicephalidae: Tomopterna)

Zootaxa ◽  
2019 ◽  
Vol 4609 (2) ◽  
pp. 225
Author(s):  
LYLE WILSON ◽  
ALAN CHANNING
Keyword(s):  
South Africa ◽  
New Species ◽  
16S Rrna ◽  
Advertisement Call ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Adult Morphology ◽  
Haplotype Networks ◽  
Tyr Gene

Tomopterna branchi sp. nov. is described from Namaqualand, South Africa. It differs from all other Tomopterna species by advertisement call, 16S rRNA sequence and consistent differences in adult morphology. The tadpole is similar to that of Tomopterna cryptotis. Haplotype networks of 16S and the nuclear tyr gene show that it is distinct from T. delalandii, with which it has been confused. A phylogeny of the genus, excluding the little-known T. monticola, shows that the new species is basal to a clade that includes T. delalandii and six other species. We extend the known range of T. damarensis to southern Namibia, and correct the identification of some GenBank material. 

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Suganthi Thevarajoo ◽  
Chitra Selvaratnam ◽  
Kian Mau Goh ◽  
Fazilah Abd. Manan ◽  
Zaharah Ibrahim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Marine Environment ◽  
Antibiotic Sensitivity ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Gram Staining ◽  
Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

Morphological and molecular characterization of cucullanid nematodes including Cucullanus opisthoporus n. sp. in freshwater fish from the Brazilian Amazon

2016 ◽  
Vol 91 (6) ◽  
pp. 739-751 ◽  
Author(s):  
F.B. Pereira ◽  
J.L. Luque
Keyword(s):  
New Species ◽  
Freshwater Fish ◽  
Original Description ◽  
Morphological Characterization ◽  
Rrna Genes ◽  
28S Rrna ◽  
Type Specimens ◽  
Tail Tip ◽  
First Time

AbstractMolecular and morphological characterization of two species of Cucullanidae from freshwater fish in Brazil are provided, one of which represented an undescribed taxon. Cucullanus opisthoporus n. sp. was collected in Cichla melaniae from River Xingu, State of Pará, and in C. pinima from River Jamarí, State of Rondônia. Nematodes referable to Cucullanus grandistomis were collected in Oxydoras niger from River Xingu. The new species has an appendage in the tail tip, ventrally covered by small spines, which is an exclusive feature of Cucullanus tucunarensis. However, C. tucunarensis differs from C. opisthoporus n. sp. based upon the relative position of deirids and the excretory pore, which are more posterior from the oesophageal end in the new species. Observations of C. tucunarensis type specimens also revealed features that were wrongly or not reported in the original description. Type specimens of C. grandistomis were observed, although they were poorly preserved. After evaluation of newly collected specimens of C. grandistomis, features unreported in the original description were observed for the first time, including the presence of an intestinal caecum. Thus, C. grandistomis was transferred to Dichelyne. Sequences of the 18S and 28S rRNA genes revealed high genetic similarity between the specimens of C. opisthoporus n. sp. from the two different hosts as well as their genetic distance from Dichelyne grandistomis n. comb. Phylogenetic reconstructions using representatives of Cucullanidae suggested the artificiality of the current morphological system adopted to separate the genera, since most genera were not monophyletic, although the availability of genetic data is still fragmented.

scholarly journals Molecular Characterization of Rhizobacteria (Isolate EO-4) as Potential Solvent of Phosphate in-vitro from the Mangrove Ecosystem of Teluk Awur, Jepara-Indonesia

2020 ◽  
Vol 5 (01) ◽  
pp. 22-33
Author(s):  
Eka Oktaviani ◽  
Rejeki Siti Ferniah ◽  
Arina Tri Lunggani
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
Molecular Characterization ◽  
Plant Growth Promoting Rhizobacteria ◽  
Mangrove Ecosystem ◽  
16S Rrna Sequence ◽  
Plant Growth Promoting ◽  
Phosphate Solubilizing

Rejuvenation and conservation of mangrove ecosystems, especially the Teluk Awur mangrove ecosystem, Jepara Regency, can be done by using bacteria that are capable of supporting plant growth or called Plant Growth Promoting Rhizobacteria (PGPR). One of the mechanisms that support plant growth by the PGPR group is phosphate dissolving activity, because the phosphate in the soil is in the form of a compound that is difficult for plants to absorb. This study aims to determine the character of Rhizobacter isolates as a superior phosphate solvent in dissolving phosphates in-vitro from the Teluk Awur mangrove ecosystem, namely isolates coded EO-4. These isolates have similar microbiological and biochemical characters to the genus Enterobacter. Molecular characterization of isolates was carried out using the Polymerase Chain Reaction (PCR) method - 16S rRNA sequence analysis (comparing with 16S rRNA sequences in gene banks). The results showed that the phosphate-solubilizing rhizobacteria that were isolated had the same base pair percentage of 48% with Enterobacter pyrinus (access number NR_028875).

scholarly journals The genetic diversity of microsymbionts from Thermopsis lanceolata growing in Mongolia

2019 ◽  
Vol 17 (1) ◽  
pp. 43-51
Author(s):  
Denis S. Karlov ◽  
Anna L. Sazanova ◽  
Irina G. Kuznetsova ◽  
Vera I. Safronova ◽  
Nina Y. Tikhomirova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
New Species ◽  
16S Rrna ◽  
Type Strain ◽  
Root Nodules ◽  
Taxonomic Position ◽  
Legume Plant ◽  
First Time ◽  
Slow Growing ◽  
Type Strains

For the first time, bacteria were isolated and identified from the root nodules of a wild-growing medicinal legume plant Thermopsis lanceolata, originated from Mongolia. The taxonomic position of 14 isolates obtained was determined using of sequencing of the 16S rRNA (rrs) and atpD genes. It was shown a significant biodiversity of the isolates from T. lanceolata, which belonged to three genera of the order Rhizobiales: Phyllobacterium (family Phyllobacteriaceae), Rhizobium (family Rhizobiaceae) and Bosea (family Bradyrhizobiaceae). Six isolates belonged to the species Phyllobacterium zundukense and Phyllobacterium trifolii (100 и 99,9% rrs similarity with the type strains P. zundukense Tri-48T and P. trifolii PETP02T, respectivelly), three isolates were identified as Rhizobium anhuiense (99,8% rrs similarity with the type strain R. anhuiense CCBAU 23252T). Two slow-growing isolates of the genus Bosea Tla-534 and Tla-545 may potentially belong to new species, since their rrs-similarity to the closest type strains B. massiliensis LMG 26221T, B. lathyri LMG 26379T and B. vaviloviae Vaf18T was 98,5-99,0%. Non-rhizobial strains were not isolated. The isolation and future investigation of the rhizobial microsymbionts of the valuable medicinal legume Thermopsis lanceolata is one of the necessary prerequisites for its industrial cultivation.

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