scholarly journals Distribution of the ermG Gene among Bacterial Isolates from Porcine Intestinal Contents

2005 ◽  
Vol 71 (8) ◽  
pp. 4930-4934 ◽  
Author(s):  
Yanping Wang ◽  
Gui-Rong Wang ◽  
Nadja B. Shoemaker ◽  
Terence R. Whitehead ◽  
Abigail A. Salyers
Keyword(s):  
Insertion Sequence ◽  
Bacillus Sphaericus ◽  
Gram Positive ◽  
Sequence Element ◽  
Conjugative Transposon ◽  
Gram Positive Bacteria ◽  
First Finding ◽  
Intestinal Contents ◽  
Porcine Feces ◽  
Insertion Sequence Element

ABSTRACT The ermG gene was first found in the soil bacterium Bacillus sphaericus. More recently, it was found in several human intestinal Bacteroides species. We report here the first finding of ermG genes in gram-positive bacteria isolated from porcine feces and from under-barn manure pits used to store porcine wastes. The porcine ermG sequences were identical to the sequence of the B. sphaericus ermG gene except that six of the seven ermG-containing strains contained an insertion sequence element insertion in the C-terminal end of the gene. The porcine ermG genes were found in three different gram-positive genera, an indication that it is possible that the gene is being spread by horizontal gene transfer. A segment of a Bacteroides conjugative transposon that carries an ermG gene cross-hybridized with DNA from six of the seven porcine isolates, but the restriction patterns in the porcine strains were different from that of the Bacteroides conjugative transposon.

scholarly journals Characterization and Functional Complementation of a Nonlethal Deletion in the Chromosome of a β-Glycosidase Mutant of Sulfolobus solfataricus

2003 ◽  
Vol 185 (13) ◽  
pp. 3948-3957 ◽  
Author(s):  
Simonetta Bartolucci ◽  
Mosè Rossi ◽  
Raffaele Cannio
Keyword(s):  
Insertion Sequence ◽  
Genomic Sequence ◽  
Sulfolobus Solfataricus ◽  
Major Facilitator Superfamily ◽  
Gene Encoding ◽  
Sequence Element ◽  
Plasmid Construct ◽  
Major Facilitator ◽  
Flanking Regions ◽  
Insertion Sequence Element

ABSTRACT LacS− mutants of Sulfolobus solfataricus defective in β-glycosidase activity were isolated in order to explore genomic instability and exploit novel strategies for transformation and complementation. One of the mutants showed a stable phenotype with no reversion; analysis of its chromosome revealed the total absence of the β-glycosidase gene (lacS). Fine mapping performed in comparison to the genomic sequence of S. solfataricus P2 indicated an extended deletion of ∼13 kb. The sequence analysis also revealed that this chromosomal rearrangement was a nonconservative transposition event driven by the mobile insertion sequence element ISC1058. In order to complement the LacS− phenotype, an expression vector was constructed by inserting the lacS coding sequence with its 5′ and 3′ flanking regions into the pEXSs plasmid. Since no transformant could be recovered by selection on lactose as the sole nutrient, another plasmid construct containing a larger genomic fragment was tested for complementation; this region also comprised the lacTr (lactose transporter) gene encoding a putative membrane protein homologous to the major facilitator superfamily. Cells transformed with both genes were able to form colonies on lactose plates and to be stained with the β-glycosidase chromogenic substrate X-Gal (5-bromo-4-chloro-3-indoyl-β-d-galactopyranoside).

scholarly journals Characterization of IS1001, an insertion sequence element of Bordetella parapertussis.

1993 ◽  
Vol 175 (1) ◽  
pp. 141-147 ◽  
Author(s):  
A van der Zee ◽  
C Agterberg ◽  
M van Agterveld ◽  
M Peeters ◽  
F R Mooi
Keyword(s):  
Insertion Sequence ◽  
Sequence Element ◽  
Bordetella Parapertussis ◽  
Insertion Sequence Element

scholarly journals Erythromycin Esterase Gene ere(A) Is Located in a Functional Gene Cassette in an Unusual Class 2 Integron

2003 ◽  
Vol 47 (10) ◽  
pp. 3326-3331 ◽  
Author(s):  
Latefa Biskri ◽  
Didier Mazel
Keyword(s):  
Insertion Sequence ◽  
Gene Cassette ◽  
Functional Gene ◽  
Sequence Element ◽  
Esterase Gene ◽  
Insertion Sequence Element

ABSTRACT The gene ere(A) of the plasmid pIP1100 is larger than originally reported and is organized as an integron gene cassette. The ere(A) gene cassette carries its own promoter and is propagated by a class 2 integron with an insertion sequence element, IS1, inserted upstream of the intI2 gene. The mobility of the ere(A) cassette has been demonstrated.

A new insertion sequence element containing a cfiA gene in the first imipenem-resistant Bacteroides fragilis strain isolated in Italy

2009 ◽  
Vol 34 (6) ◽  
pp. 608-609
Author(s):  
Giuliana Lo Cascio ◽  
Laura Macaccaro ◽  
Anna Rita Centonze ◽  
Jozef Sóki ◽  
Roberta Fontana ◽  
...  
Keyword(s):  
Insertion Sequence ◽  
Bacteroides Fragilis ◽  
Sequence Element ◽  
Insertion Sequence Element
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