scholarly journals Evaluation of Multiplexed Fluorescent Microsphere Immunoassay for Detection of Autoantibodies to Nuclear Antigens

2004 ◽  
Vol 11 (6) ◽  
pp. 1054-1059 ◽  
Author(s):  
Thomas B. Martins ◽  
Rufus Burlingame ◽  
Carlos A. von Mühlen ◽  
Troy D. Jaskowski ◽  
Christine M. Litwin ◽  
...  
Keyword(s):  
Lupus Erythematosus ◽  
Vascular Diseases ◽  
Disease Diagnosis ◽  
Specific Method ◽  
Serum Samples ◽  
Collagen Vascular Diseases ◽  
Fluorescent Microsphere ◽  
Nuclear Antigens ◽  
Multiplexed Fluorescent Microsphere Immunoassay ◽  
Microsphere Immunoassay

ABSTRACT Antibodies to extractable nuclear antigens (ENA) are found in a variety of collagen vascular diseases. Determining the individual specificities of these antibodies is extremely useful in establishing the disease diagnosis and in some cases the prognosis. With a multiplexed fluorescent microsphere immunoassay, reactivity to five of the most diagnostically useful ENA was measured in 249 serum samples, including samples from 56 patients previously documented to have systemic lupus erythematosus (SLE). Results of the multiplexed assay were compared to results from established ENA enzyme-linked immunosorbent assays (ELISAs), and the agreement, sensitivity, and specificity, respectively, for the five ENA evaluated were as follows: SSA, 99.1, 100.0, and 98.8%; SSB, 98.6, 88.9, and 99.5%; Sm, 97.6, 95.8, and 97.9%; RNP, 97.2, 92.7, and 98.8%; Scl-70, 93.6, 50.0, and 99.0%. In the 56 confirmed SLE patients, the frequency of significant concentrations of autoantibodies with the multiplexed assay was 21.4% for SSA, 7.1% for SSB, 10.7% for Sm, 32.1% for RNP, and 0% for Scl-70. The new flow cytometric bead-based multiplexed assay showed excellent correlation with the well-established single-analyte ELISA methods for four of five the ENA markers investigated in this study. The most notable discrepancies between the two assays were for the Scl-70 antigen, which was most often resolved in favor of the multiplexed assay. Our studies show that the multiplexed microsphere-based immunoassay is a sensitive and specific method for the detection and semiquantitation of ENA antibodies in human sera.

scholarly journals Capillaroscopic nail fold changes in collagen vascular diseases: A prospective observational study from a tertiary care teaching hospital in Western India

2021 ◽  
Vol 7 (4) ◽  
pp. 347-352
Author(s):  
Ratnakar Shukla ◽  
Sheena Kapoor ◽  
Sharmila Patil ◽  
Anant Patil
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Systemic Sclerosis ◽  
Observational Study ◽  
Lupus Erythematosus ◽  
Prospective Observational Study ◽  
Raynaud’S Phenomenon ◽  
Tertiary Care ◽  
Vascular Diseases ◽  
Systemic Lupus ◽  
Collagen Vascular Diseases

: To examine nail changes with capillaroscopy in patients with collagen vascular diseases. In this prospective observational study, patients with collagen vascular diseases presented to dermatology outpatient department of a tertiary care center were subjected to capillaroscopy. Structural and morphological abnormalities in these patients were noted. Fifty patients (Mean age 38.2 years; 86% females) with mean disease duration of 1.96 years were included. Patients with systemic sclerosis and systemic lupus erythematosus constituted 34% and 32% patients respectively. Twenty two (44%) patients had presence of dilated nail fold capillary loops. Out of 17 patients with systemic sclerosis, 15(88.23%) and 14(87.5%) showed dilated nail fold capillary loops and capillary drop-pouts respectively. Capillary dropouts were observed in 14(82.35%) patients. Raynaud’s phenomenon, splinter hemorrhage and fingertip scarring was observed in 11(64.71%), six (35.29%) and 33(17.65%) patients respectively. Ragged cuticle was observed in four (25%) patients with systemic lupus erythematosus. In our study, systemic sclerosis and systemic lupus erythematosus were two most common disorders. Dilated nail fold capillary loops/giant capillaries were the most common observation in overall population. Dilated nail fold capillary loops, capillary dropouts, Raynaud’s phenomenon, splinter hemorrhage and fingertip scarring were common in patients with systemic sclerosis.

scholarly journals Determination of Cytokine Responses Using a Multiplexed Fluorescent Microsphere Immunoassay

2002 ◽  
Vol 118 (3) ◽  
pp. 346-353 ◽  
Author(s):  
Thomas B. Martins ◽  
Brian M. Pasi ◽  
Jerry W. Pickering ◽  
Troy D. Jaskowski ◽  
Christine M. Litwin ◽  
...  
Keyword(s):  
Fluorescent Microsphere ◽  
Cytokine Responses ◽  
Multiplexed Fluorescent Microsphere Immunoassay ◽  
Microsphere Immunoassay

scholarly journals Subacute Cutaneous Lupus Erythematosus-Like Drug Eruption Related to Terbinafine in a Male

2021 ◽  
Vol 5 (4) ◽  
pp. 437-440
Author(s):  
Haley Danielle Heibel ◽  
Sidra Ibad ◽  
Parneet Dhaliwal ◽  
Sharif Currimbhoy ◽  
Clay J. Cockerell
Keyword(s):  
Lupus Erythematosus ◽  
Cutaneous Lupus Erythematosus ◽  
Connective Tissue Diseases ◽  
Vascular Diseases ◽  
Future Research ◽  
Subacute Cutaneous Lupus Erythematosus ◽  
Drug Induced ◽  
Collagen Vascular Diseases ◽  
History Of ◽  
Cutaneous Lupus

        Patients with a history of or a predisposition to autoimmune or collagen vascular diseases are inclined to develop drug-induced subacute cutaneous lupus erythematosus (SCLE) due to terbinafine therapy.  Here, we report a case of terbinafine-induced SCLE in a male patient, although classic SCLE most commonly affects females, and he did not have a diagnosis of or a history suggestive of a predisposition to autoimmune or collagen vascular diseases.  Although the mechanism for terbinafine-induced SCLE has not been fully elucidated, we suggest that there may be distinctive mechanisms of terbinafine-induced SCLE of patients with and without a predisposition to or history of autoimmune or connective tissue diseases, which should be a focus of future research.

scholarly journals Seroprevalence of SARS-CoV-2 Antibodies Among Rural Healthcare Workers

2021 ◽  
Author(s):  
Jordan Z Neises ◽  
Hossain Md Saddam ◽  
Rifat Sultana ◽  
Kevin N Wanniarachchi ◽  
Jared W Wollman ◽  
...  
Keyword(s):  
South Dakota ◽  
Healthcare Workers ◽  
Virus Neutralization ◽  
Serum Samples ◽  
Public Health Agencies ◽  
Fluorescent Microsphere ◽  
Previous Diagnosis ◽  
Health Agencies ◽  
Study Population ◽  
Microsphere Immunoassay

The objective of this longitudinal cohort study was to determine the seroprevalence of antibodies to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in healthcare workers employed at healthcare clinics in three rural counties in eastern South Dakota and western Minnesota from May 13, 2020 through December 22, 2020. Three blood draws were performed at five clinical sites and tested for the presence of antibodies against the SARS-CoV-2 virus. Serum samples were tested for the presence of antibodies using a fluorescent microsphere immunoassay (FMIA), neutralization of SARS-CoV-2 Spike-pseudotyped particles (SARS-CoV-2pp) assay, and serum virus neutralization (SVN) assay. The seroprevalence was determined to be 1/336 (0.29%) for samples collected from 5/13/20-7/13/20, 5/260 (1.92%) for samples collected from 8/13/20-9/25/20, and 35/235 (14.89%) for samples collected from 10/16/20-12/22/20. Eight of the 35 (22.8%) seropositive individuals identified in the final draw did not report a previous diagnosis with COVID-19. There was a high correlation (>90%) among the FMIA and virus neutralization assays. Each clinical site's seroprevalence was higher than the cumulative incidence for the general public in each respective county as reported by state public health agencies. As of December 2020, there was a high percentage (85%) of seronegative individuals in the study population.

MR Imaging of Rheumatic Diseases Affecting the Pediatric Population

2021 ◽  
Vol 25 (01) ◽  
pp. 082-093
Author(s):  
Eva Schiettecatte ◽  
Lennart Jans ◽  
Jacob Lester Jaremko ◽  
Min Chen ◽  
Caroline Vande Walle ◽  
...  
Keyword(s):  
Rheumatic Diseases ◽  
Lupus Erythematosus ◽  
Pediatric Population ◽  
Vascular Diseases ◽  
Unknown Origin ◽  
Chronic Recurrent Multifocal Osteomyelitis ◽  
Joint Inflammation ◽  
Response To Treatment ◽  
Collagen Vascular Diseases ◽  
Juvenile Spondyloarthritis

AbstractThis article reviews the application of magnetic resonance imaging (MRI) to pediatric rheumatic diseases. MRI can detect early manifestations of arthritis, evaluate the extent of disease, and monitor disease activity and response to treatment.Juvenile idiopathic arthritis (JIA) is the most common pediatric rheumatic disorder, representing a diverse group of related diseases that share a definition of joint inflammation of unknown origin with onset before 16 years of age and lasting > 6 weeks. JIA may lead to significant functional impairment and is increasingly imaged with MRI to assess for active inflammation as a target for therapy. This is particularly true for juvenile spondyloarthritis that includes multiple subgroups of JIA and primarily involves the spine and sacroiliac joints.Other less common pediatric rheumatic diseases considered here are chronic recurrent multifocal osteomyelitis and collagen vascular diseases including polymyositis, dermatomyositis, scleroderma, and juvenile systemic lupus erythematosus.

scholarly journals A Multiplexed Fluorescent Microsphere Immunoassay for Antibodies to Pneumococcal Capsular Polysaccharides

2002 ◽  
Vol 117 (4) ◽  
pp. 589-596 ◽  
Author(s):  
Jerry W. Pickering ◽  
Thomas B. Martins ◽  
Ryan W. Greer ◽  
M. Carl Schroder ◽  
Mark E. Astill ◽  
...  
Keyword(s):  
Capsular Polysaccharides ◽  
Fluorescent Microsphere ◽  
Multiplexed Fluorescent Microsphere Immunoassay ◽  
Microsphere Immunoassay

A CASE OF TUBERCULOUS TENOSYNOVITIS IN A PATIENT WITH SYSTEMIC LUPUS ERYTHEMATOSUS

Hand Surgery ◽  
2004 ◽  
Vol 09 (01) ◽  
pp. 109-113 ◽  
Author(s):  
M. Oshima ◽  
A. Fukui ◽  
Y. Takakura
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Steroid Therapy ◽  
Lupus Erythematosus ◽  
Histopathological Examination ◽  
Vascular Diseases ◽  
Steroid Treatment ◽  
Systemic Lupus ◽  
Collagen Vascular Diseases ◽  
Increased Risk ◽  
Tuberculous Tenosynovitis

A 52-year-old woman with systemic lupus erythematosus (SLE) was infected with tuberculosis (TB) on her forearm and hand, after 16 years of steroid therapy. Debridements and anti-TB therapy were performed successfully. Recently, the risk of significant morbidity from TB has been on the rise; this appears to be a complication of steroid therapy used to treat AIDS and some collagen vascular diseases. It is thought that steroid therapy causes an increased risk of TB. In this paper, we report our experience of this SLE patient who developed tuberculous tenosynovitis. We suggest that TB infection must be considered in the differential diagnosis whenever a patient presents with a chronic wrist or hand inflammation that is non-responsive to steroid treatment. Once TB infection is suspected, both histopathological and bacteriological examinations should be performed. Emergent treatment includes surgical debridement and the institution of early anti-TB therapy immediately after completing histopathological examination.

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