Analysis of the 16S rRNA Gene Sequence of Anaplasma centrale and Its Phylogenetic Relatedness to Other Ehrlichiae
2001 ◽
Vol 8
(2)
◽
pp. 241-244
◽
Keyword(s):
16S Rrna
◽
ABSTRACT The nucleotide sequence of the Anaplasma centrale 16S rRNA gene was determined and compared with the sequences of ehrlichial bacteria. The sequence of A. centrale was closely related to Anaplasma marginale by both level-of-similarity (98.08% identical) and distance analysis. A species-specific PCR was developed based upon the alignment data. The PCR can detect A. centrale DNA extracted from 10 infected bovine red blood cells in a reaction mixture. A. centrale DNA was amplified in the reaction, but not other related ehrlichial species.
1999 ◽
Vol 48
(6)
◽
pp. 723-739
◽
2006 ◽
Vol 56
(12)
◽
pp. 2765-2770
◽