Use of Protein-Specific Monoclonal Antibody-Based Latex Agglutination for Rapid Diagnosis of Burkholderia pseudomallei Infection in Patients with Community-Acquired Septicemia

ABSTRACT A latex agglutination test employing monoclonal antibody specific to a 30-kDa protein of Burkholderia pseudomallei was used to detect the organisms in blood culture specimens from 1,139 patients with community-acquired septicemia. The sensitivity, specificity, and positive and negative predictive values of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively.

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Rapid Identification of Burkholderia pseudomallei by Latex Agglutination Based on an Exopolysaccharide-Specific Monoclonal Antibody

Journal of Clinical Microbiology ◽

10.1128/jcm.37.1.225-228.1999 ◽

1999 ◽

Vol 37 (1) ◽

pp. 225-228 ◽

Cited By ~ 31

Author(s):

I. Steinmetz ◽

A. Reganzerowski ◽

B. Brenneke ◽

S. Häussler ◽

A. Simpson ◽

...

Keyword(s):

Monoclonal Antibody ◽

Southeast Asia ◽

Burkholderia Pseudomallei ◽

Rapid Identification ◽

Latex Agglutination ◽

Agglutination Test ◽

Latex Agglutination Test ◽

Specific Method ◽

Northern Australia ◽

Specific Monoclonal Antibody

We recently identified a constitutively expressed exopolysaccharide of Burkholderia pseudomallei which is composed of a unique linear tetrasaccharide repeating unit consisting of three galactose residues and one 3-deoxy-d-manno-2-octulosonic acid residue. In this study we developed a latex agglutination test based on monoclonal antibody 3015, which is specific for this exopolysaccharide, and evaluated this test for rapid identification ofB. pseudomallei grown on agar plates. All 74 environmental and clinical B. pseudomallei strains tested, originating from different areas of Southeast Asia, northern Australia, and Africa, showed a strong and specific agglutination. B. pseudomallei-like organisms and a variety of other bacteria did not react. In conclusion this monoclonal antibody-based test is a simple, rapid, and highly specific method for identifying B. pseudomallei culture isolates from different geographic areas.

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Short report: evaluation of a monoclonal antibody-based latex agglutination test for rapid diagnosis of septicemic melioidosis.

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.1999.61.735 ◽

1999 ◽

Vol 61 (5) ◽

pp. 735-737 ◽

Cited By ~ 20

Author(s):

N Samosornsuk ◽

S Sirisinha ◽

A Lulitanond ◽

N Saenla ◽

N Anuntagool ◽

...

Keyword(s):

Monoclonal Antibody ◽

Latex Agglutination ◽

Rapid Diagnosis ◽

Agglutination Test ◽

Latex Agglutination Test ◽

Short Report

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Detection of urinary leishmanial antigen by latex agglutination test (KAtex) in kala-azar patients

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v9i4.6688 ◽

1970 ◽

Vol 9 (4) ◽

pp. 216-222 ◽

Cited By ~ 1

Author(s):

Md Abdus Salam ◽

Dinesh Mondal ◽

Mamun Kabir ◽

Rashidul Haque

Keyword(s):

Visceral Leishmaniasis ◽

Confidence Interval ◽

Medical Science ◽

Latex Agglutination ◽

Agglutination Test ◽

Latex Agglutination Test ◽

Healthy Controls ◽

Predictive Values ◽

Non Invasive ◽

Kala Azar

Background: A new unique latex agglutination test (KAtex) that detects a stable, nonprotein, disease specific parasite antigen in the freshly voided urine of patients suffering from active kala-azar has been introduced by Kalon Biological Ltd. UK. This is absolutely non-invasive method of diagnosis for visceral leishmaniasis and suitable for implementation as a rapid diagnostic tool at the point of care. Objective: Diagnostic potential of KAtex was evaluated among clinically suspected kala-azar patients in an endemic zone of Bangladesh. Methodology: KAtex was done using freshly voided urine according to the manufacturer’s instructions for sixty (60) clinically suspected patients of kala-azar admitted in Rajshahi Medical College Hospital (RMCH), Bangladesh and forty (40) healthy controls during December 2005 to June 2006. Leishmania nested Polymerase Chain Reaction (Ln-PCR) using peripheral blood buffy coat was performed for all study population (100) and Ln-PCR positive cases were considered as confirmed cases of kalaazar. Results: Out of 60 clinically suspected kala-azar patients, 56 were Ln-PCR positive and 53 of 56 Ln-PCR positive cases were KAtex positive (sensitivity, 94.64%; Mantel-Haenszel Chi sq. 79.66, p= 0.0000, confidence interval [CI], >95 to 100%). None of the healthy controls was found positive by Ln-PCR but 2 of 40 were KAtex positive (specificity, 95%; confidence interval [CI], >95 to 100%). The positive and negative predictive values of KAtex were noted as 98.10% and 92.85% respectively. Conclusion: This limited prospective study suggests that KAtex is an absolutely non-invasive urinebased antigen detection test with high sensitivity and specificity and may be useful for screening active kala-azar patients, particularly suitable for field use. Key words: Visceral leishmaniasis; Kala-azar; KAtex; Ln-PCR; sensitivity; specificity. DOI: 10.3329/bjms.v9i4.6688Bangladesh Journal of Medical Science Vol.09 No.4 July 2010 pp.216-222

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