scholarly journals Evaluation of Oral Fluid Enzyme Immunoassay for Confirmation of a Positive Rapid Human Immunodeficiency Virus Test Result

2009 ◽  
Vol 16 (7) ◽  
pp. 1091-1092 ◽  
Author(s):  
L. G. Wesolowski ◽  
T. Sanchez ◽  
D. A. MacKellar ◽  
B. M. Branson ◽  
S. F. Ethridge ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Enzyme Immunoassay ◽  
Oral Fluid ◽  
False Negative ◽  
Test Results ◽  
Human Immunodeficiency Virus Test ◽  
Hiv Test ◽  
Rapid Hiv Test ◽  
Immunodeficiency Virus ◽  
Test Result

ABSTRACT The CDC recommends that a reactive rapid human immunodeficiency virus (HIV) test be confirmed with an approved supplemental test; the performance of an intermediate enzyme immunoassay (EIA) is optional. In support of this recommendation, it was found that of 1,431 reactive rapid HIV test results, 2 (0.1%) had false-negative oral fluid Western blot results and both had false-negative EIA results.

scholarly journals Efficacy of a Less-Sensitive Enzyme Immunoassay (3A11-LS) for Early Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Infants

2001 ◽  
Vol 8 (6) ◽  
pp. 1282-1285
Author(s):  
Debra Candal ◽  
Marc Bulterys ◽  
Elaine J. Abrams ◽  
Richard W. Steketee ◽  
Bharat S. Parekh
Keyword(s):  
Human Immunodeficiency Virus ◽  
Early Diagnosis ◽  
Enzyme Immunoassay ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunodeficiency Virus ◽  
Hiv Antibodies ◽  
Test Result ◽  
Rule Out

ABSTRACT We evaluated a less-sensitive enzyme immunoassay (3A11-LS) for its possible use for early diagnosis of human immunodeficiency virus type 1 (HIV-1) infection in infants. The results were compared with those from the immunoglobulin G-capture enzyme immunoassay. A total of 239 sera from 77 infants were tested. All 25 sera from the 10 infants born to seronegative mothers were found to be negative by both assays. Forty-one seroreverting infants showed a complete decay of maternal antibodies by 4 months by the 3A11-LS assay. However, the assay detected HIV antibodies in only 9 (36%) of 25 sera collected from infected infants between 4 and 6 months and in 27 (63%) of 43 sera collected after 6 months of age. Further analysis with alternative cutoff values indicated that the 3A11-LS had a sensitivity of 12 to 44% and a specificity of 90 to 100% for infants between 4–6 months of age. This data suggest that a diagnosis of HIV infection in some of the infants could be made after 4 months of age by the 3A11-LS assay, although a negative 3A11-LS test result may not rule out infection and may require a further followup.

scholarly journals Social Media–Based Secondary Distribution of Human Immunodeficiency Virus/Syphilis Self-testing Among Chinese Men Who Have Sex with Men

2020 ◽  
Author(s):  
Dan Wu ◽  
Yi Zhou ◽  
Nancy Yang ◽  
Shanzi Huang ◽  
Xi He ◽  
...  
Keyword(s):  
Social Media ◽  
Human Immunodeficiency Virus ◽  
Test Results ◽  
Self Testing ◽  
Immunodeficiency Virus ◽  
Secondary Distribution ◽  
Self Test ◽  
Sex With Men ◽  
Test Result ◽  
Unique Index

Abstract Background Social media and secondary distribution (distributing self-testing kits by indexes through their networks) both show strong promise to improve human immunodeficiency virus (HIV) self-testing uptake. We assessed an implementation program in Zhuhai, China, which focused on the secondary distribution of HIV/syphilis self-test kits among men who have sex with men (MSM) via social media. Methods Men aged ≥16 years, born biologically male, and ever had sex with another man were recruited as indexes. Banner ads on a social media platform invited the participants to apply for up to 5 self-test kits every 3 months. Index men paid a deposit of US$15/kit refundable upon submitting a photograph of a completed test result via an online submission system. They were informed that they could distribute the kits to others (referred to as “alters”). Results A total of 371 unique index men applied for 1150 kits (mean age, 28.7 [standard deviation, 6.9] years), of which 1141 test results were returned (99%). Among them, 1099 were valid test results; 810 (74%) were from 331 unique index men, and 289 tests (26%) were from 281 unique alters. Compared to index men, a higher proportion of alters were naive HIV testers (40% vs 21%; P < .001). The total HIV self-test reactivity rate was 3%, with alters having a significantly higher rate than indexes (5% vs 2%; P = .008). A total of 21 people (3%) had a reactive syphilis test result. Conclusions Integrating social media with the secondary distribution of self-test kits may hold promise to increase HIV/syphilis testing coverage and case identification among MSM.

scholarly journals Evaluation and Diagnostic Usefulness of Domestic and Imported Enzyme-Linked Immunosorbent Assays for Detection of Human Immunodeficiency Virus Type 1 Antibody in India

2005 ◽  
Vol 12 (12) ◽  
pp. 1425-1428 ◽  
Author(s):  
H. Syed Iqbal ◽  
Suniti Solomon ◽  
K. G. Murugavel ◽  
Sunil Suhas Solomon ◽  
P. Balakrishnan
Keyword(s):  
Human Immunodeficiency Virus ◽  
False Negative ◽  
Confirmatory Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Testing ◽  
Negative Results ◽  
Hiv Test ◽  
Immunodeficiency Virus ◽  
Hiv Antibodies ◽  
Hiv 1

ABSTRACT Diagnosis of human immunodeficiency virus (HIV) infection is important for patient management and prevention of new infections. The number of test kits available for the detection of HIV antibodies is unprecedented. In order to identify appropriate test kits, we evaluated a variety of commercial kits manufactured abroad as well as in India. The plasma and serum specimens (n = 264) were collected from individuals attending the Voluntary Counseling and Testing Centre at the YRG Centre for AIDS and Education. The specimens were used to evaluate six commercially available HIV test kits: Enzaids HIV 1+2, HIV-CheX, Murex HIV-1.2.0, Genscreen HIV 1/2 version 2, Vironostika HIV Uni-Form II Ag/Ab, and CombAids RS Advantage. High sensitivities and specificities (≥99%) were observed for the Enzaids, Murex, Vironostika, and CombAids assays. HIV-CheX showed the highest number of false-positive and false-negative results. The Genscreen test also gave many false positives. The study indicated that the Enzaids, Murex, and Vironostika enzyme-linked immunosorbent assay kits and the CombAids RS Advantage rapid assay could be used to achieve acceptable results for the detection of HIV antibodies. A combination of two tests is recommended to optimize the efficiency of HIV antibody testing algorithms, especially when evaluation with an HIV Western blot confirmatory test is not possible.

Repeatedly False-Negative Rapid HIV Test Results in a Patient with Undiagnosed Advanced AIDS

2008 ◽  
Vol 149 (1) ◽  
pp. 71 ◽  
Author(s):  
Patricia Brown ◽  
Joseph R. Merline ◽  
Diane Levine ◽  
Lucio Román Minces
Keyword(s):  
False Negative ◽  
Test Results ◽  
Hiv Test ◽  
Rapid Hiv Test
Sign in / Sign up

Export Citation Format

Share Document