scholarly journals Evaluation of Serological Tests To Identify Trypanosoma cruzi Infection in Humans and Determine Cross-Reactivity with Trypanosoma rangeli and Leishmania spp.

2007 ◽  
Vol 14 (8) ◽  
pp. 1045-1049 ◽  
Author(s):  
Zuleima C. Caballero ◽  
Octavio E. Sousa ◽  
Waldelania P. Marques ◽  
Amadeo Saez-Alquezar ◽  
Eufrosina S. Umezawa
Keyword(s):  
Trypanosoma Cruzi ◽  
Synthetic Peptides ◽  
Cross Reactivity ◽  
Reference Test ◽  
Trypanosoma Rangeli ◽  
Serological Tests ◽  
Western Blot Assay ◽  
Leishmania Spp ◽  
Cruzi Infection ◽  
Secreted Antigens

ABSTRACT Five commercially available enzyme-linked immunosorbent assays (ELISAs), one in-house ELISA, and two hemagglutination assays were evaluated to determine their diagnostic accuracy for Chagas' disease in two studies. In study 1, ELISA kits showed 100% sensitivity, but specificities ranged from 82.84% to 100% when leishmaniasis cases were included and from 95.57% to 100% when leishmaniasis cases were excluded. Kits using recombinant antigens or synthetic peptides are more specific than those using crude extracts from Trypanosoma cruzi epimastigote forms. Kits evaluated in Panama, in study 2, showed 75% to 100% sensitivity and 97.12% to 100% specificity. These data were obtained by using a Western blot assay with T. cruzi trypomastigote excreted-secreted antigens as a reference test to confirm T. cruzi infection.

scholarly journals Etiological treatment of young women infected with Trypanosoma cruzi, and prevention of congenital transmission

2009 ◽  
Vol 42 (5) ◽  
pp. 484-487 ◽  
Author(s):  
Sergio Sosa-Estani ◽  
Estela Cura ◽  
Elsa Velazquez ◽  
Cristina Yampotis ◽  
Elsa Leonor Segura
Keyword(s):  
Trypanosoma Cruzi ◽  
Young Women ◽  
Serological Tests ◽  
Congenital Transmission ◽  
Cruzi Infection ◽  
Efficacy Of Treatment

The objective was to detect Trypanosoma cruzi infection in 32 children in Salta, Argentina, born to 16 chronically infected young women who were treated with benznidazole. Tests were performed to assess the efficacy of treatment after 14 years. At the end of the follow up, 87.5% of the women were non-reactive to EIA tests, 62.5% to IHA and 43.8% to IFA. 62.5% of the women were non-reactive according to two or three serological tests. No infected children were detected among the newborns of mothers treated before their pregnancy.

scholarly journals New evidence of spontaneous cure in human Chagas' disease

2003 ◽  
Vol 36 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Sonia S. Francolino ◽  
Antonio Fernandez Antunes ◽  
Rodolfo Talice ◽  
Rachel Rosa ◽  
Joel Selanikio ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Acute Phase ◽  
Clinical Data ◽  
Specific Treatment ◽  
Serological Tests ◽  
Spontaneous Cure ◽  
New Evidence ◽  
Cruzi Infection

A new case of spontaneous cure of human Chagas' disease is described in Uruguay. An 87-year-old man who had a typical acute phase of Trypanosoma cruzi infection in 1947 and never received specific treatment against the disease, when examined in 1998 revealed several completely negative parasitological and serological tests, including traditional serology, PCR and flow cytometry. As a whole, such findings fulfill the current criteria to define the cure of Chagas' disease. Clinical data suggest the possibility of a benign evolution of Chagas' disease in this case, but the basic findings (slight cardiac and esophageal impairment) could also be due to the advanced age of the patient.

scholarly journals Course of Chronic Trypanosoma cruzi Infection after Treatment Based on Parasitological and Serological Tests: A Systematic Review of Follow-Up Studies

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0139363 ◽  
Author(s):  
Yanina Sguassero ◽  
Cristina B. Cuesta ◽  
Karen N. Roberts ◽  
Elizabeth Hicks ◽  
Daniel Comandé ◽  
...  
Keyword(s):  
Systematic Review ◽  
Trypanosoma Cruzi ◽  
Serological Tests ◽  
Follow Up Studies ◽  
Cruzi Infection

scholarly journals Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera

2008 ◽  
Vol 1 (1) ◽  
pp. 20 ◽  
Author(s):  
Milene H de Moraes ◽  
Alessandra A Guarneri ◽  
Fabiana P Girardi ◽  
Juliana B Rodrigues ◽  
Iriane Eger ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Cross Reactivity ◽  
Trypanosoma Rangeli

scholarly journals Trypanosoma cruzi Infection in an Indigenous Kariña Community in Eastern Venezuela

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Mariolga Berrizbeitia ◽  
Dairene Moreno ◽  
Brian J. Ward ◽  
Erika Gómez ◽  
Alicia Jorquera ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Indigenous Population ◽  
Serum Samples ◽  
Factors Associated ◽  
Indirect Haemagglutination ◽  
Elisa Format ◽  
History Of ◽  
The Mean ◽  
Cruzi Infection ◽  
Secreted Antigens

We investigated the seroprevalence of Trypanosoma cruzi infection in an indigenous Kariña population in eastern Venezuela. A total of 175 serum samples were collected in the community of Piñantal during February 2009. Interviews targeting socioeconomic and environmental factors associated with the T. cruzi transmission were also conducted. Samples were evaluated using trypomastigote excreted/secreted antigens (TESAs) in an ELISA format. TESA-ELISA positive samples were confirmed by indirect haemagglutination (HAI) (Wiener). A nonsystematic collection of vectors was also undertaken. T. cruzi seroprevalence was 7.43% according to both assays, and the mean age of infected patients was 48.61±10.40 years (range 34 to 73 years). The vector infection rate was 20.00% (2/10). T. cruzi seropositivity was associated with a history of triatomine bites, the ability to recognize the vector and poor knowledge about Chagas disease, but no associations were found with gender, house type, knowledge of how the disease is transmitted, or the presence of vectors or animals inside dwellings. To our knowledge, this is the first study of the seroprevalence of T. cruzi in an indigenous population in eastern Venezuela. All of the epidemiological variables required for the establishment of active vectorial transmission of T. cruzi were present in this community.

Geographic variations in test reactivity for the serological diagnosis of Trypanosoma cruzi infection

2021 ◽  
Author(s):  
Carine Truyens ◽  
Eric Dumonteil ◽  
Jackeline Alger ◽  
Maria Luisa Cafferata ◽  
Alvaro Ciganda ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Test Performance ◽  
Elisa Test ◽  
Standard Test ◽  
Human Populations ◽  
Geographic Variations ◽  
Serological Tests ◽  
Rapid Tests ◽  
Antibody Levels ◽  
Cruzi Infection

Chagas disease is a neglected disease caused by Trypanosoma cruzi parasites. Most of diagnosis is based on serological tests but the lack of a gold standard test complicates the measurement of test performance. To overcome this limitation, we used samples from a cohort of well-characterized T. cruzi infected women to evaluate the reactivity of two rapid diagnostic tests and one ELISA assay. Our cohort derived from a previous study on congenital transmission of T. cruzi , and consisted in 481 blood/plasma samples from Argentina (n=149), Honduras (n=228) and Mexico (n=104) with at least one positive T. cruzi PCR. Reactivity of the three tests ranged from 70.5% for the Wiener ELISA to 81.0% for the T-Detect and 90.4% for the Stat-Pak rapid tests. Test reactivity varied significantly among countries, and was highest in Argentina, and lowest in Mexico. When considering at least two reactive serological tests to confirm seropositivity, over 12% of T. cruzi infection cases from Argentina were missed by serological tests, over 21% in Honduras, and an alarming 72% in Mexico. Differences in test performance among countries were not due to differences in parasitemia, but differences in antibody levels against ELISA test antigens were observed. Geographic differences in T. cruzi parasite strains as well as genetic differences among human populations may both contribute to the discrepancies in serological testing. Improvements in serological diagnostics for T. cruzi infections are critically needed to ensure an optimum identification of cases.

scholarly journals trans-Sialidase Neutralizing Antibody Detection in Trypanosoma cruzi-Infected Domestic Reservoirs

2011 ◽  
Vol 18 (6) ◽  
pp. 984-989 ◽  
Author(s):  
Paula A. Sartor ◽  
Martha V. Cardinal ◽  
Marcela M. Orozco ◽  
Ricardo E. Gürtler ◽  
M. Susana Leguizamón
Keyword(s):  
Visceral Leishmaniasis ◽  
Trypanosoma Cruzi ◽  
Neutralizing Antibodies ◽  
Cross Reactivity ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Trypanosoma Rangeli ◽  
Serum Samples ◽  
Dipstick Test ◽  
Content Type

ABSTRACTThe detection ofTrypanosoma cruziinfection in domestic dogs and cats is relevant to evaluating human transmission risks and the effectiveness of insecticide spraying campaigns. However, the serological assays routinely used are associated with cross-reactivity in sera from mammals infected withLeishmaniaspp. We used atrans-sialidase inhibition assay (TIA) forT. cruzidiagnosis in serum samples from 199 dogs and 57 cats from areas where these types of infections are endemic. TIA is based on the antibody neutralization of recombinanttrans-sialidase, an enzyme that is not detected in the coendemicLeishmaniaspecies orTrypanosoma rangeliparasites.T. cruziinfection was also evaluated by conventional serology (CS) (indirect immunofluorescence, indirect hemagglutination, enzyme-linked immunosorbent assay, and immunochromatographic dipstick test) and xenodiagnosis. Sera from 30 dogs and 15 cats from areas where these organisms are not endemic and 5 dogs with visceral leishmaniasis were found to be nonreactive by TIA and CS. Samples from dogs and cats demonstrated 91 and 95% copositivities between TIA and CS, whereas the conegativities were 98 and 97%, respectively. Sera from xenodiagnosis-positive dogs and cats also reacted by TIA (copositivities of 97 and 83%, respectively). TIA was reactive in three CS-negative samples and was able to resolve results in two cat serum samples that were CS inconclusive. Our study is the first to describe the development oftrans-sialidase neutralizing antibodies in naturally infected dogs and cats. High CS conegativity and the absence oftrans-sialidase neutralization in dog sera from areas where leishmaniasis is not endemic and from dogs with visceral leishmaniasis support TIA specificity. The TIA may be a useful tool forT. cruzidetection in the main domestic reservoirs.

Sign in / Sign up

Export Citation Format

Share Document