scholarly journals Cross-Reactivity between Immune Responses to Helicobacter bilis and Helicobacter pylori in a Population in Thailand at High Risk of Developing Cholangiocarcinoma

2008 ◽  
Vol 15 (9) ◽  
pp. 1363-1368 ◽  
Author(s):  
Paola Pisani ◽  
Mark T. Whary ◽  
Ingrid Nilsson ◽  
Supannee Sriamporn ◽  
Torkel Wadström ◽  
...  
Keyword(s):  
High Risk ◽  
Immune Responses ◽  
Cross Reactivity ◽  
Surface Proteins ◽  
Massachusetts Institute Of Technology ◽  
Whole Cell ◽  
Serologic Tests ◽  
Helicobacter Bilis ◽  
Institute Of Technology ◽  
H Pylori

ABSTRACT Helicobacter bilis DNA has been detected in human tissue and is a candidate for etiologic investigations on the causes of hepatic and biliary tract diseases, but reliable serologic tests need to be developed in order to pursue such investigations. The scope of this study was to assess the specificity of two assays for H. bilis immune response allowing for H. pylori, and their cross-reactivity in a population in Thailand at high risk for cholangiocarcinoma. Plasma samples from 92 Thai volunteers were independently tested in two laboratories (Massachusetts Institute of Technology [MIT] and Lund). MIT performed three analyses of H. pylori and H. bilis based either on (i) outer membrane protein (OMP) with no preabsorption or on antigens derived from whole-cell sonicate before (ii) or after (iii) preabsorption with H. pylori sonicate protein. Lund used cell surface proteins from H. pylori and H. bilis as antigens. Testing for H. bilis was preabsorbed with a whole-cell lysate of H. pylori. More than 80% of the samples were positive for H. pylori in both laboratories. As tested by MIT, 58.7% (95% confidence interval, 47.9 to 68.9%) were positive for H. bilis by OMP and 44.5% (34.1 to 55.3%) were positive for H. bilis sonicate protein, but only 15.2% (8.6 to 24.2%) remained positive after preabsorption with H. pylori sonicate protein. Lund found 34.5% of the samples positive for H. bilis (22.0 to 41.0%), which was statistically compatible with all three MIT results. Serologic responses to OMPs of the two bacteria coincided in 66 and 45% of the samples in the MIT and Lund assays, respectively. We found high cross-reactivity between the immune responses to H. pylori and H. bilis antigens. More-specific H. bilis antigens need to be isolated to develop serologic tests suitable for epidemiological studies.

Serologic detection ofBrachyspira (Serpulina) hyodysenteriaeinfections

2001 ◽  
Vol 2 (1) ◽  
pp. 45-52 ◽  
Author(s):  
T. La ◽  
D.J. Hampson
Keyword(s):  
Cross Reactivity ◽  
Whole Cell ◽  
Brachyspira Hyodysenteriae ◽  
Serologic Tests ◽  
Cell Surface Structures ◽  
Outer Membrane Lipoprotein ◽  
Membrane Lipoprotein ◽  
Different Strains ◽  
Serologic Assay ◽  
Important Disease

AbstractSwine dysentery (SD) caused by the intestinal spirocheteBrachyspira hyodysenteriaeis an economically important disease in pig-producing countries throughout the world. To date, no specific serologic assay is commercially available for the diagnosis of pigs with SD. Several serologic techniques have been identified in the past; however, these tests have all used either whole-cell proteins or lipopolysaccharide (LPS) as the antigen. Whole-cell antigens are plagued with false-positive reactions due to cross-reactivity with common proteins shared with other spirochetes. LPS antigens produce fewer false-positives; however, false-negatives may result due to LPS components being serogroup-specific. Generally, these techniques are useful for detecting infected herds, but are unreliable for the detection of individual infected pigs. In order to develop improved serologic tests it will be necessary to identify suitable diagnostic antigens, in particular immunogenic cell-surface structures which are specific toB. hyodysenteriaebut common amongst different strains of the species. Recently, we identified and cloned a 30-kDa outer membrane lipoprotein (BmpB) which is specific toB. hyodysenteriaeand is recognized by experimentally and naturally infected pigs. In this review we summarize the available serologic tests for SD, and speculate on the use of recombinant BmpB as an antigen for future development of an improved serologic test for SD diagnosis.

scholarly journals Characterization of Proteins in the Outer Membrane Preparation of a Murine Pathogen, Helicobacter bilis

2001 ◽  
Vol 69 (5) ◽  
pp. 3502-3506 ◽  
Author(s):  
Zhongming Ge ◽  
Peter Doig ◽  
James G. Fox
Keyword(s):  
Outer Membrane ◽  
Wide Spectrum ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Cross Reactivity ◽  
Membrane Preparation ◽  
Laboratory Animals ◽  
Helicobacter Bilis ◽  
H Pylori

ABSTRACT Helicobacter bilis is a bacterial pathogen associated with multifocal hepatitis and inflammatory bowel disease in certain strains of mice. This bacterium colonizes the liver, bile, and lower intestine in mice and has also been isolated from a wide spectrum of laboratory animals. In this study, proteins present in the outer membrane preparation (OMP) of four H. bilis strains isolated from a mouse, a dog, a rat, and a gerbil were characterized and compared with that of Helicobacter pylori, a human gastric pathogen. All four H. bilis strains had similar OMP protein profiles that were distinct from those of H. pylori. Immunoblotting demonstrated that OMP proteins fromH. bilis and H. pylori have little cross-reactivity, except for their flagellins. Nine major immunogenic polypeptides were present in the H. bilis OMPs. By using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, five heat-modifiable proteins with molecular masses of 82, 66, 52, 47 and 37 kDa were identified. The N-terminal sequences of the 46- and 47-kDa OMP proteins had no homology with protein sequences available in public databases. These results indicate that H. bilis has a conserved, unique OMP protein profile that is distinct from those of H. pylori.

scholarly journals Identification of immunodominant Helicobacter pylori proteins with reactivity to H. pylori-specific egg-yolk immunoglobulin

2003 ◽  
Vol 52 (3) ◽  
pp. 217-222 ◽  
Author(s):  
Ji-Hyun Shin ◽  
Seung-Woo Nam ◽  
Jung-Taik Kim ◽  
Jong-Bok Yoon ◽  
Won-Gi Bang ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Egg Yolk ◽  
Cross Reactivity ◽  
Heat Shock Protein 60 ◽  
Α Subunit ◽  
Whole Cell ◽  
Whole Cell Lysate ◽  
Cell Lysate ◽  
Normal Human ◽  
H Pylori

The importance of hens eggs as a source of specific antibodies (IgY) is well recognized. The protective effect of IgY obtained from hens immunized with Helicobacter pylori whole-cell lysate has been reported for the control of H. pylori infection. However, IgY produced by whole-cell lysates presents the possibility of cross-reactivity with other bacteria, including the normal human flora, and this could decrease the efficiency of IgY. In the present study, the immunodominant proteins of H. pylori with reactivity to H. pylori-specific IgY (IgY-Hp) were identified. IgY obtained from hens immunized with various fractions of H. pylori proteins was isolated and purified, titres of IgY-Hp against H. pylori were determined and cross-reactivity between IgY-Hp and normal human bacteria was examined by Western blot analysis. Finally, immunodominant H. pylori proteins were identified by LC/MS analysis. IgY obtained 2 months after immunization with H. pylori whole-cell lysate showed the highest antibody titre. Five immunodominant proteins were identified that were strongly reactive to IgY-Hp: urease β-subunit (62 kDa), heat-shock protein 60 (60 kDa), urease α-subunit (26 kDa), probable peroxiredoxin (22 kDa) and probable thiol peroxidase (18 kDa). Immunization of hens with the immunodominant proteins identified would produce a more specific IgY against H. pylori.

scholarly journals Analysis of Humoral Immune Responses to Surface and Virulence-Associated Chlamydia abortus Proteins in Ovine and Human Abortions by Use of a Newly Developed Line Immunoassay

2016 ◽  
Vol 54 (7) ◽  
pp. 1883-1890 ◽  
Author(s):  
Jürgen Benjamin Hagemann ◽  
Ulrike Simnacher ◽  
David Longbottom ◽  
Morag Livingstone ◽  
Julia Maile ◽  
...  
Keyword(s):  
Immune Responses ◽  
Specific Antigen ◽  
Surface Antigens ◽  
Laboratory Diagnosis ◽  
Cross Reactivity ◽  
Infected Sheep ◽  
Surface Proteins ◽  
Expression Library ◽  
Content Type ◽  
Zoonotic Risk

The obligate intracellular bacteriumChlamydiaabortusis the causative agent of enzootic abortion of ewes and poses a significant zoonotic risk for pregnant women. Using proteomic analysis and gene expression library screening in a previous project, we identified potential virulence factors and candidates for serodiagnosis, of which nine were scrutinized here with a strip immunoassay. We have shown that aborting sheep exhibited a strong antibody response to surface (MOMP, MIP, Pmp13G) and virulence-associated (CPAF, TARP, SINC) antigens. While the latter disappeared within 18 weeks following abortion in a majority of the animals, antibodies to surface proteins persisted beyond the duration of the study. In contrast, nonaborting experimentally infected sheep developed mainly antibodies to surface antigens (MOMP, MIP, Pmp13G), all of which did not persist. We were also able to detect antibodies to these surface antigens inC.abortus-infected women who had undergone septic abortion, whereas a group of shepherds and veterinarians with occupational exposure toC.abortus-infected sheep revealed only sporadic immune responses to the antigens selected. The most specific antigen for the serodiagnosis of humanC.abortusinfections was Pmp13G, which showed no cross-reactivity with other chlamydiae infecting humans. We suggest that Pmp13G-based serodiagnosis accomplished by the detection of antibodies to virulence-associated antigens such as CPAF, TARP, and SINC may improve the laboratory diagnosis of human and animalC.abortusinfections.

Applying the Anderson-Darling Test to Suicide Clusters

Crisis ◽  
2013 ◽  
Vol 34 (6) ◽  
pp. 434-437 ◽  
Author(s):  
Donald W. MacKenzie
Keyword(s):  
Poisson Process ◽  
Goodness Of Fit ◽  
Small Samples ◽  
Massachusetts Institute Of Technology ◽  
Homogeneous Poisson Process ◽  
Cornell University ◽  
The Difference ◽  
Suicide Clusters ◽  
Institute Of Technology ◽  
Anderson Darling

Background: Suicide clusters at Cornell University and the Massachusetts Institute of Technology (MIT) prompted popular and expert speculation of suicide contagion. However, some clustering is to be expected in any random process. Aim: This work tested whether suicide clusters at these two universities differed significantly from those expected under a homogeneous Poisson process, in which suicides occur randomly and independently of one another. Method: Suicide dates were collected for MIT and Cornell for 1990–2012. The Anderson-Darling statistic was used to test the goodness-of-fit of the intervals between suicides to distribution expected under the Poisson process. Results: Suicides at MIT were consistent with the homogeneous Poisson process, while those at Cornell showed clustering inconsistent with such a process (p = .05). Conclusions: The Anderson-Darling test provides a statistically powerful means to identify suicide clustering in small samples. Practitioners can use this method to test for clustering in relevant communities. The difference in clustering behavior between the two institutions suggests that more institutions should be studied to determine the prevalence of suicide clustering in universities and its causes.

Editorial: Advancing the Debate on Architecture, Planning, and Built Environment Research

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Ashraf M. Salama
Keyword(s):  
Learning Styles ◽  
Cultural Values ◽  
Urban Studies ◽  
Wide Spectrum ◽  
Journal Ranking ◽  
Massachusetts Institute Of Technology ◽  
Open Access Journals ◽  
Practice Based Research ◽  
Institute Of Technology ◽  
Number Of Citations

With an acceptance rate that does not exceed 25% of the total papers and articles submitted to the journal, IJAR – International Journal of Architectural Research is moving forward to position itself among the leading journals in architecture and urban studies worldwide. As this is the case since the beginning of volume 5, issue 1, March 2011, one must note that the journal has been covered by several data and index bases since its inception including Avery Index to Architectural Periodicals, EBSCO-Current Abstracts-Art and Architecture, INTUTE, Directory of Open Access Journals, Pro-Quest, Scopus-Elsevier and many university library databases across the globe. This is coupled with IJAR being an integral part of the archives and a featured collection of ArchNet and the Aga Khan Documentation Centre at MIT: Massachusetts Institute of Technology, Cambridge, MA.In 2014, IJAR was included in Quartile 2 / Q2 list of Journals both in ‘Architecture’ and ‘Urban Studies.’ As of May 2015, IJAR is ranked 23 out of 83 journals in ‘Architecture’ and 59 out of 119 in ‘Urban Studies.’ Rankings are based on the SJR (SCImago Journal Ranking); an Elsevier- SCOPUS indicator that measures the scientific influence of the average article in a journal. SJR is a measure of scientific influence of scholarly journals that accounts for both the number of citations received by a journal and the importance or prestige of the journals where such citations come from. See here for more information (http://www.scimagojr.com/index.php) and (http://www.journalmetrics.com/sjr.php). While the journal is now on top of many of the distinguished journals in Elsevier- SCOPUS database, we will keep aspiring to sustain our position and move forward to Q1 group list and eventually in the top 10 journal list in the field. However, this requires sustained efforts and conscious endeavours that give attention to quality submissions through a rigorous review process. This edition of IJAR: volume 9, issue 2, July 2015 includes debates on a wide spectrum of issues, explorations and investigations in various settings. The issue encompasses sixteen papers addressing cities, settlements, and projects in Europe, South East Asia, and the Middle East. Papers involve international collaborations evidenced by joint contributions and come from scholars in universities, academic institutions, and practices in Belgium; Egypt; Greece; Italy; Jordan; Malaysia; Palestine; Qatar; Saudi Arabia; Serbia; Spain; Turkey; and the United Kingdom. In this editorial I briefly outline the key issues presented in these papers, which include topics relevant to social housing, multigenerational dwelling, practice-based research, sustainable design and biomimetic models, learning environments and learning styles, realism and the post modern condition, development and planning, urban identity, contemporary landscapes, and cultural values and traditions.

XX Rudi Dombusch, Keys to Prosperity. Free Markets, Sound Money, and bit of Luck The MIT Press 2000 Londres

2009 ◽  
Vol 33 (130) ◽  
Author(s):  
GERARDO REYES GUZMÁN
Keyword(s):  
Massachusetts Institute ◽  
Massachusetts Institute Of Technology ◽  
Free Markets ◽  
Institute Of Technology

Rudiger Dornbusch, destacado economista del Massachusetts Institute of Technology (MIT), analiza en esta trascendental obra tópicos como inflación, deuda, tipos de cambio, política externa y mercados emergentes. El marco conceptual descansa en la corriente de la escuela de Chicago, la cual parte del principio de que el mercado es el mecanismo que garantiza la creación del progreso en contraste con el Estado, que en su afán por encontrar soluciones perfectas, fracasa regularmente en sus cometidos.

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