Serological Diagnosis of Lung Cystic Hydatid Disease Using the Synthetic p176 Peptide

ABSTRACTCystic hydatid disease (CHD) is a worldwide zoonosis caused by the larval stage of the dog tapewormEchinococcus granulosus. Diagnosis is based on imagenological tools (abdominal ultrasound, chest X-rays, or computed tomography [CT] scan). Serological antibody-detecting assays, using diverse native antigens, have been used as a supportive diagnostic tool, but their sensitivities and specificities differ greatly. The use of synthetic peptides as antigens should provide more reliability and allow better assessment and comparison of test formats and case series. The synthetic peptide p176, corresponding to the N-terminal extreme of the subunit of antigen B (AgB8/1), has shown promising performances for diagnosis of CHD. We evaluated the performance of the synthetic peptide p176 for the diagnosis of pulmonary hydatid disease in an enzyme-linked immunosorbent assay (ELISA) format. Sixty-one serum samples from patients with a diagnosis of pulmonary hydatidosis confirmed by surgery and 128 from healthy volunteers were tested. The overall sensitivity and specificity of the p176 ELISA for lung CHD were 78.69% and 96.88%, respectively. On bivariate analysis, positive serum antibody reactions were associated with the presence of complications and with the number of cysts (single/multiple). Only the presence of persistent complications significantly associated with seropositivity on multivariate logistic regression analysis (odds ratio [OR], 9.58; 95% confidence interval [CI], 2.15 to 42.6;P= 0.003). The p176 ELISA performs well for the diagnosis of lung CHD and adds an easily reproducible diagnostic assay to the existing diagnostic tools.

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An enzyme-linked immunosorbent assay using an arc 5 antigen for the diagnosis of cystic hydatid disease

Annals of Tropical Medicine and Parasitology ◽

10.1080/00034983.1990.11812449 ◽

1990 ◽

Vol 84 (2) ◽

pp. 157-162 ◽

Cited By ~ 16

Author(s):

P. R. Hira ◽

G. M. Bahr ◽

H. M. Shweiki ◽

K. Behbehani

Keyword(s):

Hydatid Disease ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Cystic Hydatid Disease ◽

Cystic Hydatid

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Cystic hydatid disease (Echinococcus granulosus)

Oxford Textbook of Medicine ◽

10.1093/med/9780198746690.003.0180 ◽

2020 ◽

pp. 1529-1533

Author(s):

Pedro L. Moro ◽

Hector H. Garcia ◽

Armando E. Gonzalez

Keyword(s):

Echinococcus Granulosus ◽

Hydatid Disease ◽

Imaging Techniques ◽

Clinical Manifestations ◽

Public Health Problem ◽

Major Public Health Problem ◽

Cystic Hydatid Disease ◽

Control Programmes ◽

Domestic Livestock ◽

Cystic Hydatid

Cystic hydatid disease, caused by Echinococcus granulosus, is a zoonotic disease principally transmitted between dogs and domestic livestock, particularly sheep. Humans are infected when they ingest tapeworm eggs, with disease occurring in most parts of the world where sheep are raised and dogs are used to herd livestock. The most common clinical manifestations are cysts in the liver (typically presenting with hepatomegaly) and/or lung (presenting with cough, haemoptysis, and dyspnoea). Diagnosis is usually made on the basis of serological tests in combination with imaging techniques. Treatment options include surgery, chemotherapy with anthelminthic agents, or—for liver cysts—PAIR (puncture–aspiration–injection–reaspiration). Echinococcosis is a major public health problem in several countries. Control programmes have been aimed at educating dog owners to prevent their animals from having access to infected offal. Vaccines against sheep hydatidosis and the dog tapeworm stage are promising alternatives.

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Praziquantel: Neglected drug? Ineffective treatment? Or therapeutic choice in cystic hydatid disease?

Acta Tropica ◽

10.1016/j.actatropica.2009.04.006 ◽

2009 ◽

Vol 111 (2) ◽

pp. 95-101 ◽

Cited By ~ 50

Author(s):

J.M. Bygott ◽

P.L. Chiodini

Keyword(s):

Hydatid Disease ◽

Cystic Hydatid Disease ◽

Therapeutic Choice ◽

Cystic Hydatid

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Cystic Hydatid Disease in Southern Sudan

Annals of African Surgery ◽

10.4314/aas.v2i1.46238 ◽

2009 ◽

Vol 2 (1) ◽

Author(s):

DK Lado

Keyword(s):

Hydatid Disease ◽

Cystic Hydatid Disease ◽

Southern Sudan ◽

Cystic Hydatid

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Oxfendazole Treatment for Cystic Hydatid Disease in Naturally Infected Animals

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.42.3.601 ◽

1998 ◽

Vol 42 (3) ◽

pp. 601-605 ◽

Cited By ~ 23

Author(s):

Ronald E. Blanton ◽

Timothy M. Wachira ◽

Eberhard E. Zeyhle ◽

Ernest M. Njoroge ◽

Japheth K. Magambo ◽

...

Keyword(s):

Hydatid Disease ◽

Cyst Wall ◽

Inflammatory Cells ◽

Chemotherapeutic Agents ◽

Postmortem Examination ◽

Cystic Hydatid Disease ◽

Adventitial Layer ◽

Cystic Hydatid

ABSTRACT Few chemotherapeutic agents are available for the medical management of hydatid disease caused by the parasite Echinococcus granulosus. In order to test the potential of oxfendazole for the treatment of infection with this parasite, nine infected goats and four sheep were given oxfendazole twice weekly at a dose of 30 mg/kg of body weight for 4 weeks and monitored by ultrasound for an additional 4 weeks. Efficacy was finally evaluated by postmortem examination, including determination of protoscolex viability and cyst wall histology. In treated animals, protoscolices were dead or absent in 97% of cysts from oxfendazole-treated animals compared to 28% of cysts from untreated control animals. On postmortem examination, 53% of cysts from treated animals were found to be grossly degenerate. A sample of those cysts that appeared potentially viable all demonstrated evidence of severe damage to the cyst wall. By light microscopy, cysts showed severe disorganization of the adventitial layer with invasion of inflammatory cells and in some cases frank necrosis with no apparent adventitial layer. The follow-up period for assessment of the drug’s ability to cause complete degeneration and resorption of cysts was relatively short. This study, however, indicates that oxfendazole is at least as effective as and is easier to administer than albendazole for the treatment of hydatid disease.

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