scholarly journals Complete Genome Sequence of Nervous Necrosis Virus Isolated from Orange-Spotted Grouper (Epinephelus coioides) in Taiwan

2017 ◽  
Vol 5 (37) ◽  
Author(s):  
Ming-Hui Chien ◽  
Thi Tuyet Minh Vo ◽  
Shaw-Yun Wu ◽  
Cheng-Hui Lin
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Sequence ◽  
Virus Strain ◽  
Viral Genome ◽  
Nervous Necrosis Virus ◽  
Epinephelus Coioides ◽  
Necrosis Virus ◽  
Virus Genotype ◽  
Rna Molecules ◽  
Positive Sense

ABSTRACT The genome sequence of nervous necrosis virus strain KS1 isolated from orange-spotted grouper (Epinephelus coioides) was cloned and analyzed. The viral genome is composed of two single-stranded positive-sense RNA molecules, RNA1 and RNA2. Phylogenetic analysis shows that the virus strain KS1 belongs to the red-spotted grouper nervous necrosis virus genotype.

scholarly journals Near-Complete Genome Sequence of a Fish Nervous Necrosis Virus Isolated from Hybrid Grouper in China

2020 ◽  
Vol 9 (15) ◽  
Author(s):  
Peng Jia ◽  
Xiaoqi Chen ◽  
Jiajie Fu ◽  
Meisheng Yi ◽  
Wenbo Chen ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus ◽  
Virus Genotype ◽  
Content Type ◽  
Hybrid Grouper ◽  
Epinephelus Fuscoguttatus

The genome sequence of nervous necrosis virus strain HGN1910, isolated from hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂), was cloned, sequenced, and characterized. Two near-complete gene segments were obtained, RNA1 and RNA2. Phylogenetic analysis shows that the virus belongs to the red-spotted grouper nervous necrosis virus genotype of betanodavirus.

scholarly journals Near-Complete Genome Sequence of a Fish Nervous Necrosis Virus Isolated from a Clinical Disease Outbreak in Farm-Reared Bream Sparus aurata in Spain

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Panagiota Stathopoulou ◽  
Nausika Rafailidou ◽  
Kostas Tzokas ◽  
Costas Batargias ◽  
George Tsiamis
Keyword(s):  
Phylogenetic Tree ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Viral Genome ◽  
Complete Genome ◽  
Sparus Aurata ◽  
Disease Outbreak ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus ◽  
Viral Nervous Necrosis

ABSTRACT Sparus aurata larvae infected by viral nervous necrosis were collected from an aquaculture fish farm. The isolated viral genome, composed of two segments (RNA1 and RNA2), was sequenced and analyzed comparatively. Phylogenetic tree analyses revealed that the isolated strain is a reassortant, exhibiting a red-spotted grouper nervous necrosis virus (RGNNV)-type RNA1 and a striped jack nervous necrosis virus (SJNNV)-type RNA2.

scholarly journals Complete Genome Sequence of a Zika Virus Strain Isolated from the Serum of an Infected Patient in Thailand in 2006

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Narong Nitatpattana ◽  
Kumchol Chaiyo ◽  
Supoth Rajakam ◽  
Kanya Poolam ◽  
Kusuma Chansiprasert ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Sequence ◽  
Virus Strain ◽  
Zika Virus ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Infected Patient ◽  
Vero Cells ◽  
Asian Lineage

ABSTRACT The complete genome of Zika virus (ZIKV) strain CVD_06-274 was isolated from the serum of an infected patient in Thailand in 2006. Phylogenetic analysis showed that this strain belongs to the Asian lineage and also high titers in Vero cells (RCB 10-87). It has potential for development as an inactivated ZIKV vaccine.

scholarly journals Betanodavirus infection in Kuhlia rupestris and Ambassis marianus and isolation of betanodavirus from infected pond water

2020 ◽  
Vol 142 ◽  
pp. 1-11
Author(s):  
K Agnihotri ◽  
R Chong ◽  
D Underwood ◽  
C Kistler ◽  
M Hutchison
Keyword(s):  
Phylogenetic Analysis ◽  
Nucleotide Sequence ◽  
Cell Line ◽  
Environmental Samples ◽  
Sanger Sequencing ◽  
High Throughput Sequencing ◽  
Pond Water ◽  
Fish Cell ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus

This is the first report of betanodavirus infection in 2 species of finfish, Kuhlia rupestris (jungle perch) and Ambassis marianus (estuary perchlet). This report also describes isolation of betanodavirus from infected pond water using the SSN-1 cell line. Histopathology of K. rupestris larvae revealed vacuolation in the eye and brain, which was confirmed using betanodavirus-specific immunohistochemistry. The eye and brain from A. marianus and betanodavirus isolated from pond water were confirmed using real-time PCR and Sanger sequencing. High throughput sequencing was used to obtain betanodavirus sequences from paraffin blocks containing infected K. rupestris. The phylogenetic analysis of betanodavirus RNA1 and RNA2 sequences from all 3 sources were associated with the red-spotted grouper nervous necrosis virus (RGNNV) genotype. The RNA1 nucleotide sequence from jungle perch showed 100% identity with the betanodavirus water isolate and 99.37% identity with A. marianus. Furthermore, we have demonstrated the usefulness of combining recovery of viable virus from environmental samples through fish cell line culture with PCR testing as a means of validating the efficacy of chlorination to eradicate betanodavirus from the pond environment.

scholarly journals Nervous Necrosis Virus Coat Protein Mediates Host Translation Shutoff through Nuclear Translocalization and Degradation of Polyadenylate Binding Protein

2021 ◽  
Author(s):  
Chao-An Cheng ◽  
Jia-Ming Luo ◽  
Ming-Hsien Chiang ◽  
Kuei-Yuan Fang ◽  
Chen-Hung Li ◽  
...  
Keyword(s):  
Coat Protein ◽  
Marine Fish ◽  
Molecular Mechanisms ◽  
Fish Larvae ◽  
Binding Protein ◽  
Economic Damage ◽  
Brain Cells ◽  
Nervous Necrosis Virus ◽  
Epinephelus Coioides ◽  
Necrosis Virus

Nervous necrosis virus (NNV) belongs to the Betanodavirus genus of the Nodaviridae family and is the main cause of viral nervous necrosis disease in marine fish larvae and juveniles worldwide. The NNV virion contains two positive-sense, single-stranded RNA genomes, which encode RNA-dependent RNA polymerase, coat protein and B2 protein. Interestingly, NNV infection can shut off host translation in orange-spotted grouper ( Epinephelus coioides ) brain cells, however, the detailed mechanisms of this action remain unknown. In this study, we discovered that the host translation factor, polyadenylate binding protein (PABP), is a key target during NNV takeover of host translation machinery. Additionally, ectopic expression of NNV coat protein is sufficient to trigger nuclear translocalization and degradation of PABP, followed by translation shutoff. A direct interaction between NNV coat protein and PABP was demonstrated, and this binding requires the NNV coat protein N-terminal shell domain and PABP proline-rich linker region. Notably, we also showed that degradation of PABP during later stages of infection is mediated by the ubiquitin-proteasome pathway. Thus, our study reveals that the NNV coat protein hijacks host PABP, causing its relocalization to the nucleus and promoting its degradation to stimulate host translation shutoff. IMPORTANCE Globally, more than 200 species of aquacultured and wild marine fish are susceptible to NNV infection. Devastating outbreaks of this virus have been responsible for massive economic damage in the aquaculture industry, but the molecular mechanisms by which NNV affects its host remain largely unclear. In this study, we show that NNV hijacks translation in host brain cells, with the viral coat protein binding to host PABP to promote its nuclear translocalization and degradation. This previously unknown mechanism of NNV-induced host translation shutoff greatly enhances the understanding of NNV pathogenesis and provides useful insights and novel tools for development of NNV treatments, such as the use of orange-spotted grouper brain cells as an in vitro model system.

A BIVALENT INACTIVATED VACCINE OF VIRAL NERVOUS NECROSIS VIRUS AND GROUPER IRIDOVIRUS APPLIED TO GROUPER BROODFISH (EPINEPHELUS COIOIDES) REDUCES THE RISK OF VERTICAL TRANSMISSION

2017 ◽  
Vol 43 (03) ◽  
pp. 171-176 ◽  
Author(s):  
Sue-Min Huang ◽  
Jin-Hua Cheng ◽  
Chien Tu ◽  
Tzyy-Ing Chen ◽  
Chun-Ta Lin ◽  
...  
Keyword(s):  
Vertical Transmission ◽  
Neutralizing Antibodies ◽  
High Titer ◽  
Serum Antibody ◽  
Pcr Analysis ◽  
Nervous Necrosis Virus ◽  
Epinephelus Coioides ◽  
Necrosis Virus ◽  
Viral Nervous Necrosis ◽  
Level 1

Forty-one broodfish of orange-spotted groupers (Epinephelus coioides) were selected to evaluate the effectiveness of a viral nervous necrosis virus (VNNV) and grouper iridovirus (GIV) inactivated bivalent vaccine in grouper broodfish. Real-time quantitative PCR analysis showed that a detection rate of 10.5% (2/19) was found in egg specimens of VNNV and GIV, which carried approximately 1780 copies of GIV viral DNA in the egg specimens from broodfish before vaccination. This confirmed the vertical transmission route of GIV in broodfish. A significant increase of the anti-VNNV serum antibody titer was more than 50% in the high titer level (1:1810 to 1:5120) and 45% in the moderate titer level (1:452 to 1:1280), which were higher than those of the anti-GIV display, with 50% (10/20) in a titer of 1:57 to 1:320 and 40% (8/20) in a titer of 1:452 to 1:1280 one month after the vaccination. This result showed that the VNNV is a highly antigenic virus and can effectively induce neutralizing antibodies better than GIV. In addition, the VNNV and GIV viral copy numbers were 97.1 and 1780 copies per [Formula: see text]g host egg DNA from the broodfish before vaccination, respectively. One month after the vaccination, the viral genomes of VNNV and GIV were undetectable in egg specimens. The results show that immunization can induce the production of specific protective neutralizing antibodies, and the infective antigens can thereby be eliminated by the immunity. The results demonstrate that the specific antibodies of GIV and VNNV induced by vaccination can reduce the risk of vertical transmission of VNNV and GIV in grouper broodfish.

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