scholarly journals Genome Sequences of Subcluster K5 Mycobacteriophages AlleyCat, Edugator, and Guillsminger

2017 ◽  
Vol 5 (45) ◽  
Author(s):  
Rodney A. King ◽  
Tina M. Slowan-Pomeroy ◽  
Jodi E. Thomas ◽  
Tithe Ahmed ◽  
Katie L. Alexander ◽  
...  

ABSTRACT Bacteriophages AlleyCat, Edugator, and Guillsminger were isolated on Mycobacterium smegmatis mc2155 from enriched soil samples. All are members of mycobacteriophage subcluster K5, with genomes of 62,112 to 63,344 bp. Each genome contains 92 to 99 predicted protein-coding genes and one tRNA. Guillsminger is the first mycobacteriophage to carry an IS1380 family transposon.

2017 ◽  
Vol 5 (16) ◽  
Author(s):  
Kirk R. Anders ◽  
Alex M. Murphy ◽  
William F. Ettinger ◽  
Douglas Kempthorne ◽  
Charles Kittridge ◽  
...  

ABSTRACT Mycobacteriophages DrHayes, Urkel, and SamuelLPlaqson were isolated from soil samples in Spokane, WA, using Mycobacterium smegmatis mc2155 grown at room temperature. The three genomes differ by only a few nucleotides, are 60,526 bp long, have 97 predicted protein-coding genes and one tRNA gene, and are members of subcluster K1.


2019 ◽  
Vol 8 (8) ◽  
Author(s):  
Kristofer A. Apellido ◽  
Divya Balchander ◽  
Matthew C. Erlich ◽  
Jakub K. Gocal ◽  
Wiktoria A. Gocal ◽  
...  

Twelve B1 cluster mycobacteriophages were isolated from soil samples collected in Philadelphia, PA, USA, using Mycobacterium smegmatis mc2 155 as a host, and were sequenced. The genome sequences range in size from 66,887 bp to 68,953 bp in length and have between 99 and 105 putative protein-coding genes.


2020 ◽  
Vol 9 (11) ◽  
Author(s):  
Joanna Katsanos ◽  
Chioma Ngene ◽  
Jennifer Cook-Easterwood

The mycobacteriophages Burwell21 and Nivrat are two F1 cluster bacteriophages isolated from different soil samples in Charlotte, NC. Burwell21 has a 58,098-base-pair double-stranded DNA genome, with 99 protein-coding genes predicted, whereas Nivrat has a 58,009-base-pair genome, with 102 protein-coding genes predicted.


2017 ◽  
Vol 5 (46) ◽  
Author(s):  
Christopher D. Herren ◽  
Alexandra Peister ◽  
Timothy S. Breton ◽  
Maggie S. Hill ◽  
Marcy S. Anderson ◽  
...  

ABSTRACT Four subcluster L2 mycobacteriophages, Finemlucis, Miley16, Wilder, and Zakai, that infect Mycobacterium smegmatis mc2155 were isolated. The four phages are closely related to each other and code for 12 to 14 tRNAs and 130 to 132 putative protein-coding genes, including tyrosine integrases, cro, immunity repressors, and excise genes involved in the establishment of lysogeny.


2020 ◽  
Vol 9 (34) ◽  
Author(s):  
Madison E. Davis ◽  
Véronique A. Delesalle ◽  
Kayla D. Blankenship ◽  
Jeffrey T. Good ◽  
Clayr M. Kroenke ◽  
...  

ABSTRACT Bacteriophages Awesomesauce and LastJedi infect Mycobacterium smegmatis mc2155. While the Awesomesauce genome is 57,054 bp with 94 protein-coding genes, the LastJedi genome is 55,149 bp with 94 protein-coding genes. Nucleotide sequence comparison in Phamerator detected synteny between Awesomesauce gp49 to gp61 and singleton LilSpotty. Whole-genome BLASTn alignments revealed that LastJedi strongly resembles Clifton (99.41% identity).


2019 ◽  
Vol 8 (35) ◽  
Author(s):  
A. R. Stahlke ◽  
A. Z. Ozsoy ◽  
D. W. Bean ◽  
P. A. Hohenlohe

We announce the complete circularized mitochondrial genome assemblies of Diorhabda carinata and Diorhabda carinulata, beetle species introduced to North America for the biological control of invasive shrubs of the genus Tamarix L. (Tamaricaceae). The assemblies (16,232 and 16,298 bp, respectively) each comprise 13 protein-coding genes, 22 tRNAs, two rRNAs, and a noncoding region.


2020 ◽  
Vol 9 (8) ◽  
Author(s):  
Sushanta Deb ◽  
Jhasketan Badhai ◽  
Subrata K. Das

We report the draft genome sequences of Vibrio alginolyticus strain S6-61 and Vibrio diabolicus strain S7-71, isolated from the corals Pocillopora verrucosa and Fungia danai, respectively. The genomes of strains S6-61 and S7-71 contain 4,880 and 4,641 protein coding genes, respectively, and harbor genes associated with the ectoine biosynthesis pathway.


2019 ◽  
Vol 8 (28) ◽  
Author(s):  
Dane M. Bowder ◽  
Brandon W. Gannon ◽  
Kathryn J. Grint ◽  
Jason T. Iltz ◽  
Teryn M. Koch ◽  
...  

Three mycobacteriophages, Bipolarisk, Bread, and FudgeTart, were isolated from enriched soil samples found in Crete, NE. All three phages are lytic, belong to subcluster C1, and infect Mycobacterium smegmatis mc2155. The structures of the three genomes are similar, with slight variations in gene number and content.


2020 ◽  
Vol 9 (46) ◽  
Author(s):  
Heidi Lin ◽  
Michael Reeves ◽  
Melissa Acevedo ◽  
Kelsey Bass ◽  
Elizabeth Chau ◽  
...  

ABSTRACT Cluster EC ClearAsMud and cluster EA4 Kauala are lytic Siphoviridae bacteriophages that were isolated from soil in southern California using Microbacterium foliorum NRRL B-24224 as the host. The ClearAsMud and Kauala genomes are 52,987 bp and 39,378 bp, respectively, and contain 92 and 56 predicted protein-coding genes, respectively.


2019 ◽  
Vol 8 (22) ◽  
Author(s):  
Jennifer Cook-Easterwood ◽  
Hailey Gase ◽  
Chioma Ngene ◽  
Joanna Katsanos

Ebony and Holeinone represent the first mycobacteriophages isolated from Charlotte, NC, soil samples, using the host Mycobacterium smegmatis strain mc2155. Ebony has a 52,152-bp genome and showed similarity to other phages in the A11 subcluster.


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