scholarly journals Genome Sequences of the Mycobacteriophages Awesomesauce and LastJedi

2020 ◽  
Vol 9 (34) ◽  
Author(s):  
Madison E. Davis ◽  
Véronique A. Delesalle ◽  
Kayla D. Blankenship ◽  
Jeffrey T. Good ◽  
Clayr M. Kroenke ◽  
...  

ABSTRACT Bacteriophages Awesomesauce and LastJedi infect Mycobacterium smegmatis mc2155. While the Awesomesauce genome is 57,054 bp with 94 protein-coding genes, the LastJedi genome is 55,149 bp with 94 protein-coding genes. Nucleotide sequence comparison in Phamerator detected synteny between Awesomesauce gp49 to gp61 and singleton LilSpotty. Whole-genome BLASTn alignments revealed that LastJedi strongly resembles Clifton (99.41% identity).

2017 ◽  
Vol 5 (45) ◽  
Author(s):  
Rodney A. King ◽  
Tina M. Slowan-Pomeroy ◽  
Jodi E. Thomas ◽  
Tithe Ahmed ◽  
Katie L. Alexander ◽  
...  

ABSTRACT Bacteriophages AlleyCat, Edugator, and Guillsminger were isolated on Mycobacterium smegmatis mc2155 from enriched soil samples. All are members of mycobacteriophage subcluster K5, with genomes of 62,112 to 63,344 bp. Each genome contains 92 to 99 predicted protein-coding genes and one tRNA. Guillsminger is the first mycobacteriophage to carry an IS1380 family transposon.


2017 ◽  
Vol 5 (16) ◽  
Author(s):  
Kirk R. Anders ◽  
Alex M. Murphy ◽  
William F. Ettinger ◽  
Douglas Kempthorne ◽  
Charles Kittridge ◽  
...  

ABSTRACT Mycobacteriophages DrHayes, Urkel, and SamuelLPlaqson were isolated from soil samples in Spokane, WA, using Mycobacterium smegmatis mc2155 grown at room temperature. The three genomes differ by only a few nucleotides, are 60,526 bp long, have 97 predicted protein-coding genes and one tRNA gene, and are members of subcluster K1.


2017 ◽  
Vol 5 (46) ◽  
Author(s):  
Christopher D. Herren ◽  
Alexandra Peister ◽  
Timothy S. Breton ◽  
Maggie S. Hill ◽  
Marcy S. Anderson ◽  
...  

ABSTRACT Four subcluster L2 mycobacteriophages, Finemlucis, Miley16, Wilder, and Zakai, that infect Mycobacterium smegmatis mc2155 were isolated. The four phages are closely related to each other and code for 12 to 14 tRNAs and 130 to 132 putative protein-coding genes, including tyrosine integrases, cro, immunity repressors, and excise genes involved in the establishment of lysogeny.


2021 ◽  
Vol 10 (26) ◽  
Author(s):  
Christine A. Byrum ◽  
Hannah Marie Rozier ◽  
Toni E. Allison ◽  
Emilia Ballou ◽  
Lauren Bergen ◽  
...  

Lolalove, a B4 subcluster soil bacteriophage of Mycobacterium smegmatis , was isolated in Charleston, South Carolina. It possesses a 71,111-bp linear double-stranded DNA (dsDNA) genome with 99 protein-coding genes and a GC content of 68.9%. Whole-genome BLASTn alignments indicate high sequence identity to the related B4 subcluster M. smegmatis phages BrownCNA, Mithril, and Hangman.


2019 ◽  
Vol 8 (8) ◽  
Author(s):  
Kristofer A. Apellido ◽  
Divya Balchander ◽  
Matthew C. Erlich ◽  
Jakub K. Gocal ◽  
Wiktoria A. Gocal ◽  
...  

Twelve B1 cluster mycobacteriophages were isolated from soil samples collected in Philadelphia, PA, USA, using Mycobacterium smegmatis mc2 155 as a host, and were sequenced. The genome sequences range in size from 66,887 bp to 68,953 bp in length and have between 99 and 105 putative protein-coding genes.


2019 ◽  
Vol 8 (35) ◽  
Author(s):  
A. R. Stahlke ◽  
A. Z. Ozsoy ◽  
D. W. Bean ◽  
P. A. Hohenlohe

We announce the complete circularized mitochondrial genome assemblies of Diorhabda carinata and Diorhabda carinulata, beetle species introduced to North America for the biological control of invasive shrubs of the genus Tamarix L. (Tamaricaceae). The assemblies (16,232 and 16,298 bp, respectively) each comprise 13 protein-coding genes, 22 tRNAs, two rRNAs, and a noncoding region.


2020 ◽  
Vol 9 (18) ◽  
Author(s):  
Rita Zgheib ◽  
Hussein Anani ◽  
Didier Raoult ◽  
Pierre-Edouard Fournier

In 2007, Salirhabdus euzebyi was first described as a bacterial isolate from a sea salt evaporation pond. As no genome sequence was previously available for this species, we performed whole-genome sequencing. The chromosome of strain Q1438 was 3,784,443 bp long with 36% G+C content, 3,830 protein-coding genes, and 74 RNA genes.


2020 ◽  
Vol 9 (8) ◽  
Author(s):  
Sushanta Deb ◽  
Jhasketan Badhai ◽  
Subrata K. Das

We report the draft genome sequences of Vibrio alginolyticus strain S6-61 and Vibrio diabolicus strain S7-71, isolated from the corals Pocillopora verrucosa and Fungia danai, respectively. The genomes of strains S6-61 and S7-71 contain 4,880 and 4,641 protein coding genes, respectively, and harbor genes associated with the ectoine biosynthesis pathway.


2017 ◽  
Vol 5 (22) ◽  
Author(s):  
Madeline R. Galac ◽  
Jason Stam ◽  
Rosslyn Maybank ◽  
Mary Hinkle ◽  
Dietrich Mack ◽  
...  

ABSTRACT Staphylococcus epidermidis 1457 is a frequently utilized strain that is amenable to genetic manipulation and has been widely used for biofilm-related research. We report here the whole-genome sequence of this strain, which encodes 2,277 protein-coding genes and 81 RNAs within its 2.4-Mb genome and plasmid.


2017 ◽  
Vol 5 (31) ◽  
Author(s):  
Shiro Kato ◽  
Tadao Oikawa

ABSTRACT Lactobacillus sakei strain LT-13 is a lactic acid bacterium isolated from moto starter of Japanese sake. This genome analysis revealed that the genome is composed of a circular chromosome and one plasmid, which contain 1,938 and 8 putative protein-coding genes, respectively.


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