scholarly journals Identification of Beak and Feather Disease Virus in an Unusual Novel Host ( Merops ornatus ) Using Nested PCR

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Subir Sarker ◽  
Shubhagata Das ◽  
Karla Helbig ◽  
Shane R. Raidal

The complete genome sequence of beak and feather disease virus (BFDV) was discovered from a rainbow bee-eater ( Merops ornatus ), a species of Coraciiformes . The genome consisted of 1,996 bp encoding two major bidirectional transcribed open reading frames. This is the first evidence of BFDV infection and complete genome characterization for this novel host species.

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Shubhagata Das ◽  
Sarker Subir ◽  
Jade K. Forwood ◽  
Seyed A. Ghorashi ◽  
Shane R. Raidal

The complete genome sequence of beak and feather disease virus (BFDV) from a fledgling red-capped parrot ( Purpureicephalus spurius ) was assembled and characterized. The genome consists of 1,995 nucleotides and encodes two major proteins in opposing directions. This is the first evidence of BFDV infectivity and a complete genome sequence for this novel host.


2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Shubhagata Das ◽  
Sarker Subir ◽  
Kathleen Fearnside ◽  
Jade K. Forwood ◽  
Seyed A. Ghorashi ◽  
...  

The complete genome sequence of beak and feather disease virus (BFDV) from a purple crowned lorikeet ( Glossopsitta porphyrocephala ) was characterized. The genome consists of 2,010 nucleotides and encodes replicase-associated protein and capsid protein. This is the first evidence of BFDV infectivity and complete genome sequence for this novel host.


Author(s):  
Zilong Zhang ◽  
Danlei Liu ◽  
Zilei Zhang ◽  
Peng Tian ◽  
Shenwei Li ◽  
...  

AbstractNorovirus is recognized as one of the leading causes of acute gastroenteritis outbreaks. Genotype GII.9 was first detected in Norfolk, VA, USA, in 1997. However, the complete genome sequence of this genotype has not yet been determined. In this study, a complete genome sequence of GII.9[P7] norovirus, SCD1878_GII.9[P7], from a patient was determined using high-throughput sequencing and rapid amplification of cDNA ends (RACE) technology. The complete genome sequence of SCD1878_GII.9[P7] is 7544 nucleotides (nt) in length with a 3’ poly(A) tail and contains three open reading frames. Sequence comparisons indicated that SCD1878_GII.9[P7] shares 92.1%-92.3% nucleotide sequence identity with GII.P7 (AB258331 and AB039777) and 96.7%-97.4% identity with GII.9 (AY038599 and DQ379715). The results suggested that SCD1878_GII.9[P7] is a member of P genotype GII.P7 and G genotype GII.9. This viral sequence fills a gap at the whole-genome level for the GII.9 genotype.


2018 ◽  
Vol 6 (7) ◽  
Author(s):  
Sondos Badran ◽  
Nathanael Morales ◽  
Phillip Schick ◽  
Brandon Jacoby ◽  
William Villella ◽  
...  

ABSTRACTBacillusspp. are ubiquitous Gram-positive microbes with many ecological and symbiotic interactions and can be pathogens. Phage Leo2 was found to infect aBacillus pumilusstrain isolated from soil. The sequence of phage Leo2 revealed 74 genes; 31% of the genes have associated functions, and 67% of coding regions are unidentified open reading frames.


2017 ◽  
Vol 5 (16) ◽  
Author(s):  
Adriana N. Souza ◽  
Fábio N. Silva ◽  
Claudine M. Carvalho

ABSTRACT A novel satellite virus of 1,228 bp in length was found in a single cassava plant. Bioinformatic analyses show that it has two open reading frames (ORFs) in its genome, probably encoding a coat protein of 156 and a putative protein of 90 amino acids.


2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Yuan-Yuan Liu ◽  
Wen-Fei Xian ◽  
Jin Xue ◽  
Yong-Lu Wei ◽  
Xiao-Wen Cheng ◽  
...  

ABSTRACT The complete genome of Trichoplusia ni ascovirus 6b (TnAV-6b) was sequenced for the first time. The TnAV-6b isolate, which has its closest phylogenetic relationship with the TnAV-6a isolate, has a circular genome of 185,664 bp, with a G+C content of 46.0% and 178 predicted open reading frames.


2021 ◽  
Vol 10 (29) ◽  
Author(s):  
Mohammad A. Tariq ◽  
Simon R. Carding

Bacteroides fragilis is an obligate anaerobe and a common gut commensal bacterium that is also an important opportunistic pathogen. Here, we present the complete genome sequence of the circularly permuted B. fragilis bacteriophage vB_BfrS_NCTC. It comprises 47,160 bp, with 69 open reading frames.


2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jakub Kubacki ◽  
Isabelle Hardmeier ◽  
Weihong Qi ◽  
Eleonora Flacio ◽  
Mauro Tonolla ◽  
...  

ABSTRACT We report here the full-length genome sequence of a rhabdovirus strain detected in a pool of 21 Culex pipiens and Culex torrentium mosquitos collected in southern Switzerland. The genome has a length of 11,914 nucleotides and encodes five major putative open reading frames.


2020 ◽  
Vol 9 (5) ◽  
Author(s):  
Guillermo Alejandro Solís-Sánchez ◽  
Evangelina Esmeralda Quiñones-Aguilar ◽  
Saul Fraire-Velázquez ◽  
Julio Vega-Arreguín ◽  
Gabriel Rincón-Enríquez

The phytopathogenic bacterium Xanthomonas vesicatoria is the causative agent of bacterial spot disease in various Solanaceae family members. Here, we describe the complete genome sequence of XaF13, a novel filamentous phage that infects the phytopathogenic bacterium X. vesicatoria. The 7,045-bp genome is predicted to encode 14 open reading frames, 7 of which are related to those of other filamentous Xanthomonas phages.


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