In vitro effect of aqueous extract of fresh cassava leaves (Manihot esculenta, Crantz) on the larvae of Haemonchus contortus as an intestinal parasite of sheep.

Cassava is frequently fed to animals. In the case of sheep, the producer relates consumption to a reduction in the parasite load. The literature has proven the effect of phenolic compounds as an anthelmintic in vivo, but no evidence for cyanogenic compounds, also present in all parts of the cassava plant, was found. A controlled in vitro bioassay was used to evaluate the aqueous extract of fresh cassava leaves. The efficiency parameter was the immobility of Haemonchus contortus larvae at the L3 stage, also used to evaluate commercial anthelmintics. Cell culture plates with 100 active L3-stage larvae per well were used, being each replicate constituted by three wells. Aqueous extract of fresh cassava leaves (FCL), Ivomec® 0.01% (PCI) as the positive control, and distilled water as negative control (NCW), were placed in the culture plate wells. Considering the immobility of the larvae as a positive anthelmintic effect, the results showed that in NCW treatments all larvae were mobile, while in PCI all 300 larvae were immobile. FCL produced a gradient of larval inactivation correlation (R2 0.996). The best-fit equation was y = -33.39ln(x) + 40.517, a logarithmic equation, which allowed the calculation of the Lethal Concentration (CL) of 3.44 µg CN- ml, or 80.0 mg of fresh cassava leaves per milliliter of water, with a performance equivalent to ivermectin. This concentration of free cyanide is compatible with the safe consumption of fresh leaves by live weight of sheep. The exact amount of cassava roots, leaves, or shoots, consumed to provide an effective dose for controlling H. contort should be established in vivo. Although phenolic compounds must also be present in the extract, the immobility was attributed to cyanogenic compounds since the correlation was proportional to the increase in the cyanide concentration. It can be concluded that the consumption of fresh cassava leaves has the potential as an anthelmintic agent to be evaluated in vivo by feeding sheep and goats. Local use could also add value to the production of fresh cassava leaves, with an average potential production of 2.5 tonnes ha-1, available throughout the year, with greater production at the beginning of cultivation and in the summer months. These leaves could be used after the roots harvesting or even after pruning for this purpose. Currently, this amount of good quality protein material remains without use in the field.

Effect of in vitro aqueous extract of fresh cassava leaves (Manihot esculenta, Crantz) on the larvae of Haemonchus contortus as intestinal parasite of sheep

Animals feeding with cassava is frequent. In the case of sheep, the producer relates consumption to a reduction in the parasite load. The literature has proven the effect of phenolic compounds as an anthelmintic in vivo, but no evidence for cyanogenic compounds, also present in all parts of the cassava plant, was found. A controlled in vitro bioassay was used to evaluate the aqueous extract of fresh cassava leaves. The efficiency parameter was the imobility of Haemonchus contortus larvae at stage L3, also used to evaluate commercial anthelmintics. Culture plates with 100 active L3 larvae per well were used, constituted a replicate of a total of three. It was placed in these wells, water extract of fresh cassava leaves (FCL) macerated in water, Ivomec® 0.01% (PCI) as positive control and distilled water as negative one (NCW). By considering the immobility of the larvae as a positive anthelmintic effect, the results showed that in NCW all larvae were mobile, while in PCI all 300 larvae were immobile. FCL produced a gradient of larval inactivation correlation (r2 0.996). The best fit equation was y = -33.39ln(x) + 40.517, a logarithmic one, which allowed to calculate the Lethal Concentration (CL) of 3. 44µg.CN-.ml, or 80.0 mg of fresh cassava leaves per milliliter, with performance equivalent to ivermectin. This concentration of free cyanide is compatible with safe consumption of fresh leaves by live weight of sheep. The exact amount of cassava roots, leaves or shoots consumed to provide an effective dose for controlling Haemonchus contort should be established in vivo. Although phenolic compounds must also be present in the extract, immobility was attributed to cyanogenic compounds since the correlation was proportional to the increase in cyanide concentration. It can be concluded that the consumption of fresh cassava leaves has potential as an anthelmintic agent to be evaluated in vivo by feeding sheep and goats. Local use could also add value to the production of fresh cassava leaves, with an average potential production of 2.5 t/ha, available throughout the year, with greater production at the beginning of cultivation and in the summer months. These leaves could be used after the roots harvesting or even in pruning for this purpose. Currently, this volume of good quality protein material is in the field, unused.

Production of bioethanol from wild cassava crude starch (Manihot glaziovii Muell. Arg) using different microbial types and fermentation times

Wild cassava (Manihot glaziovii Muell. Arg) is one of the varieties of cassava that contains toxic compounds cyanogenic glucosides, which cause unmarketable food products more suitable to be processed into bioethanol. This wild cassava plant produces tubers four times in weight compared to that of ordinary cassava, and the flesh contains about 40–70% starch by dry weight. This study aimed to determine the effect of the microbial types and fermentation times on bioethanol production from M. glaziovii Muell. Arg crude starch and to determine the type of microbes and fermentation time that can produce the highest bioethanol product from M. glaziovii Muell. Arg crude starch. This study consists of two factors using a factorial randomized block design (RBD). The first factor was the type of microbe which consists of 2 different microbes, namely R5I3 isolates and Saccharomyces cerevisiae ATCC 9763. The second factor was the fermentation time which consists of 4 levels, namely 3, 4, 5 and 6 days. The observed parameters were total dissolved solids value, pH value, reducing sugar content, and ethanol content. The data obtained were analyzed using analysis of variance (ANOVA) and continued with Tukey’s HSD post hoc tests. The results showed that fermentation using R5I3 isolates with a fermentation time of 5 days was the best treatment to obtain a maximum ethanol of 21.64±3.03 g/L, with a final pH value of 4.70±0.14, total dissolved solids 4.10±0.14°Brix and reducing sugar content of 0.91±0.02 g/L. Therefore, the new isolate R513 was highly potential for producing bioethanol from wild cassava crude starch.

Determination of Radiation Exposure and Heavy Metals Concentration in Agricultural Research Farm Federal Polytechnic Ile-Oluji and Environs

Exposure level of radiation and heavy metal concentration were examined in Agricultural research farm, Federal Polytechnic Ile-Oluji and environs using a gamma scout survey meter and Atomic Absorption Spectroscopy respectively. The exposure level was determined by placing a survey meter 1 m above the soil level in each sampling point. The mean value of exposure level was 0.5113 µSv hr-1which was higher than the recommended limit of 0.11µSv hr-1 [1]. For the heavy metal values, there is a general increase in the concentration from Zn metal to Pb in the order Zn>Fe>Cu>Cr>Cd>As>Pb. The highest concentration of heavy metals was recorded for Zn while Pb metal has the lowest heavy metal concentration for the soil samples. In the plant samples however, the trend of heavy metals is as Cu>Zn>Fe>Cr>Cd>As>Pb. The highest concentration of the metals is recorded for Cu metal while Pb has the lowest value.Concentrations of Cd, Cr, Zn and Fe were all above permissible levels in plant materials while only Pb and As have lower values than the safety limit.The transfer factor from soil to plant is greater than 0.5 in some of the areas indicating a high risk through the food chain to man. Similarly, most of the metals have transfer factors greater than 0.2 which is also an indication of contamination of the Cassava plant in the study area.

The Influence of Various Growth Regulators on Induction Organogenic Callus from Gajah and Kuning Cassava Genotype (Manihot esculenta Crantz)

Kuning and Gajah genotypes are two collections of cassava in the Biotechnology Research Center for Germplasm, LIPI with the advantages of each genotype are high beta carotene and high production. The multiplication in in vitro culture can be done one of them through organogenesis. The aim of this study was to evaluate the effect of using 2,4-D; NAA and Kinetin are used singly for the formation of organogenesis of cassava in the Kuning Cassava and Gajah genotypes. This research was conducted at the Laboratory of Molecular Genetics and Modification of Plant Biosynthetic Pathways, Biteknologi Research Center, LIPI, Bogor since January - February 2018. The source of explants were young leaves and petiols from cassava plant culture in vitro genotypes of Gajah and Kuning yam which were three months old. in culture. The basic media used as a planting medium were Murashige and Skoog (MS) media with the addition of growth regulators (ZPT) singly, 2,4-D, NAA and Kinetin with two concentrations of ZPT each, 8 and 10 mg L- 1 This research was arranged based on a completely randomized design factorial pattern consisting of 2 factors. All data obtained were analyzed using ANOVA and if there is an influence then proceed with the DMRT test with an error rate of 5% using the SPSS program. The highest number of Kuning genotype cassava organogenic callus that developed into shoots on the medium added by ZPT was 2.4 D and kinetin with the same concentration of 8 mg L-1. Formation of the best organogenic callus in petiol explants in the media with the addition of a single 2,4-D and Kinetin with the same concentration of 8 mg L-1. Keywords: Cassava, growth regulators, organogenic.

Effect of glyphosate on antibiosis in the coevolution of the pathosystem Manihot esculenta-Colletotrichum gloeosporioides

The high use of glyphosate for weed and pest control of cassava crops in Africa, particularly in Côte d'Ivoire, is correlated with the increasing extent of cassava anthracnose in the cassava producing regions. This health situation raises the question of the existence of a combined effect of glyphosate and anthracnose infection on the defence response of the cassava plant. The study was on the effect of glyphosate on antibiosis in M. esculenta in the pathosystem with C. gloeosporioides. To achieve this objective, a classical fully randomized 3-repeat device was used and antibiosis was the factor studied with 3 modalities. Treatments carried out on the plots, 4 months after planting, were inoculation of test plots of cassava with C. gloeosporoides before they were treated with 4JAI glyphosate. The assessment of phenolic and flavonoid antibiosis levels was carried out on leaf and stem organs harvested from 1JHA to 45 JHA and from the day of glyphosate treatment to 41 JHA. Comparative flavonoid antibiosis profiles were determined by CCM at the stages of maximum inhibition and maximum accumulation. Results showed that the phenolic and flavonoid antibiosis content of plants in glyphosate-treated plots decreased progressively in all varieties studied from JATr 1 to JATr 7. After the seventh day, the respective amounts of phenolic and flavonoid antibiosis gradually increased from the 8th JATr to the 180th JATr. This study showed a negative but partial interference of glyphosate in the defense mechanism of cassava cultivars (Manihot esculenta). Brou et al., 2021 Journal of Animal & Plant Sciences (J.Anim.Plant Sci. ISSN 2071-7024) Vol.48 (2): 8637-8648 https://doi.org/10.35759/JAnmPlSci.v48-2.1 8638 RESUME L’importante utilisation du glyphosate pour le désherbage et la lutte contre les adventices des cultures de manioc en Afrique et notamment en Côte d’Ivoire, et l’étendue croissante des cas d’anthracnose du manioc dans les régions de production de cette culture, soulève la question d’un possible effet conjugué du glyphosate et de l’infection à l’anthracnose. Les auteurs ont étudié l’effet du glyphosate sur les antibioses chez M. esculenta dans le pathosystème avec C. gloeosporioides. Pour atteindre cet objectif, un dispositif classique à trois répétions complètement randomisées a été utilisé et les antibioses sont le facteur étudié avec 3 modalités. Les traitements réalisés sur les parcelles, 4 mois après plant plantation, ont été l’inoculation des parcelles tests de manioc par C. gloeosporoides avant que celles-ci ne soient traitées par le glyphosate 4JAI. L’évaluation des teneurs en antibioses phénoliques et flavonoïdiques a été réalisée sur les organes feuilles et tiges récoltées de 1JAI à 45 JAI et du jour du traitement au glyphosate à 41 JAT. Les profils comparatifs en antibioses flavonoïdiques ont été déterminés par CCM aux stades de l’inhibition maximale et de l’accumulation maximale. Les résultats ont montré que la teneur en antibioses phénoliques et flavonoïdiques des plantes des parcelles traitées avec le glyphosate ont diminué progressivement chez toutes les variétés étudiées du 1er JATr au 7ème JATr. Après le septième jour, les quantités respectives des antibioses phénoliques et de flavonoïdes ont augmenté progressivement du 8ème JATr au 180ème JATr. Il ressort de cette étude, une interférence négative mais partielle du glyphosate dans le mécanisme de défense des cultivars de manioc (Manihot esculenta).

Effect of glyphosate on antibiosis in the coevolution of the pathosystem Manihot esculenta-Colletotrichum gloeosporioides

The high use of glyphosate for weed and pest control of cassava crops in Africa, particularly in Côte d'Ivoire, is correlated with the increasing extent of cassava anthracnose in the cassava producing regions. This health situation raises the question of the existence of a combined effect of glyphosate and anthracnose infection on the defence response of the cassava plant. The study was on the effect of glyphosate on antibiosis in M. esculenta in the pathosystem with C. gloeosporioides. To achieve this objective, a classical fully randomized 3-repeat device was used and antibiosis was the factor studied with 3 modalities. Treatments carried out on the plots, 4 months after planting, were inoculation of test plots of cassava with C. gloeosporoides before they were treated with 4JAI glyphosate. The assessment of phenolic and flavonoid antibiosis levels was carried out on leaf and stem organs harvested from 1JHA to 45 JHA and from the day of glyphosate treatment to 41 JHA. Comparative flavonoid antibiosis profiles were determined by CCM at the stages of maximum inhibition and maximum accumulation. Results showed that the phenolic and flavonoid antibiosis content of plants in glyphosate-treated plots decreased progressively in all varieties studied from JATr 1 to JATr 7. After the seventh day, the respective amounts of phenolic and flavonoid antibiosis gradually increased from the 8th JATr to the 180th JATr. This study showed a negative but partial interference of glyphosate in the defense mechanism of cassava cultivars (Manihot esculenta). Brou et al., 2021 Journal of Animal & Plant Sciences (J.Anim.Plant Sci. ISSN 2071-7024) Vol.48 (2): 8637-8648 https://doi.org/10.35759/JAnmPlSci.v48-2.1 8638 RESUME L’importante utilisation du glyphosate pour le désherbage et la lutte contre les adventices des cultures de manioc en Afrique et notamment en Côte d’Ivoire, et l’étendue croissante des cas d’anthracnose du manioc dans les régions de production de cette culture, soulève la question d’un possible effet conjugué du glyphosate et de l’infection à l’anthracnose. Les auteurs ont étudié l’effet du glyphosate sur les antibioses chez M. esculenta dans le pathosystème avec C. gloeosporioides. Pour atteindre cet objectif, un dispositif classique à trois répétions complètement randomisées a été utilisé et les antibioses sont le facteur étudié avec 3 modalités. Les traitements réalisés sur les parcelles, 4 mois après plant plantation, ont été l’inoculation des parcelles tests de manioc par C. gloeosporoides avant que celles-ci ne soient traitées par le glyphosate 4JAI. L’évaluation des teneurs en antibioses phénoliques et flavonoïdiques a été réalisée sur les organes feuilles et tiges récoltées de 1JAI à 45 JAI et du jour du traitement au glyphosate à 41 JAT. Les profils comparatifs en antibioses flavonoïdiques ont été déterminés par CCM aux stades de l’inhibition maximale et de l’accumulation maximale. Les résultats ont montré que la teneur en antibioses phénoliques et flavonoïdiques des plantes des parcelles traitées avec le glyphosate ont diminué progressivement chez toutes les variétés étudiées du 1er JATr au 7ème JATr. Après le septième jour, les quantités respectives des antibioses phénoliques et de flavonoïdes ont augmenté progressivement du 8ème JATr au 180ème JATr. Il ressort de cette étude, une interférence négative mais partielle du glyphosate dans le mécanisme de défense des cultivars de manioc (Manihot esculenta).

The Microbiome of Cassava (Manihot esculanta)

The plant microbiome, like the plant, influences the processes that lead to plant development, health, and crop productivity. Cassava is a perennial herbaceous plant native to South America that has been cultivated for centuries as a staple food throughout the world. Not only is cassava a good source of carbohydrates, but it also has a high tolerance for a variety of phenotypic conditions, and the majority of cassava plants are susceptible to a variety of diseases. Thus, using cassava as a model, this chapter discusses the plant microbiome. We discuss the structure and function of the microbiome, as well as the technique for studying microbiomes. Additionally, we conducted a systematic review of references pertaining to the microbiome of the cassava plant using cultivation-dependent or cultivation-independent methods. Numerous significant genera of bacteria and fungi are found in cassava’s phyllosphere and rhizosphere, including groups of gram-negative bacteria, gram-positive Actinobacteria, and gram-positive non Actinobacteria. Additionally, we identified critical organisms in the phyllosphere and rhizosphere. Cassava endophytes also produce antifungal secondary metabolites such as pumilacidins and surfactin. The investigation of their phenotypes and interactions with the cassava plant will aid in increasing productivity.