scholarly journals Characterization of the Pathogenicity of Streptococcus intermedius TYG1620 Isolated from a Human Brain Abscess Based on the Complete Genome Sequence with Transcriptome Analysis and Transposon Mutagenesis in a Murine Subcutaneous Abscess Model

2016 ◽  
Vol 85 (2) ◽  
Author(s):  
Noriko Hasegawa ◽  
Tsuyoshi Sekizuka ◽  
Yutaka Sugi ◽  
Nobuhiro Kawakami ◽  
Yumiko Ogasawara ◽  
...  
Keyword(s):  
Brain Abscess ◽  
Genome Sequence ◽  
Transcriptome Analysis ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Antimicrobial Agents ◽  
Gc Content ◽  
Streptococcus Intermedius ◽  
Content Type

ABSTRACT Streptococcus intermedius is known to cause periodontitis and pyogenic infections in the brain and liver. Here we report the complete genome sequence of strain TYG1620 (genome size, 2,006,877 bp; GC content, 37.6%; 2,020 predicted open reading frames [ORFs]) isolated from a brain abscess in an infant. Comparative analysis of S. intermedius genome sequences suggested that TYG1620 carries a notable type VII secretion system (T7SS), two long repeat regions, and 19 ORFs for cell wall-anchored proteins (CWAPs). To elucidate the genes responsible for the pathogenicity of TYG1620, transcriptome analysis was performed in a murine subcutaneous abscess model. The results suggest that the levels of expression of small hypothetical proteins similar to phenol-soluble modulin β1 (PSMβ1), a staphylococcal virulence factor, significantly increased in the abscess model. In addition, an experiment in a murine subcutaneous abscess model with random transposon (Tn) mutant attenuation suggested that Tn mutants with mutations in 212 ORFs in the Tn mutant library were attenuated in the murine abscess model (629 ORFs were disrupted in total); the 212 ORFs are putatively essential for abscess formation. Transcriptome analysis identified 37 ORFs, including paralogs of the T7SS and a putative glucan-binding CWAP in long repeat regions, to be upregulated and attenuated in vivo. This study provides a comprehensive characterization of S. intermedius pathogenicity based on the complete genome sequence and a murine subcutaneous abscess model with transcriptome and Tn mutagenesis, leading to the identification of pivotal targets for vaccines or antimicrobial agents for the control of S. intermedius infections.

scholarly journals Complete Genome Sequence of Streptococcus pneumoniae Strain Rx1, a Hex Mismatch Repair-Deficient Standard Transformation Recipient

2021 ◽  
Vol 10 (41) ◽  
Author(s):  
Anna Maria Cuppone ◽  
Lorenzo Colombini ◽  
Valeria Fox ◽  
David Pinzauti ◽  
Francesco Santoro ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Mismatch Repair ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
Open Reading Frames ◽  
Circular Chromosome ◽  
Content Type ◽  
Sequencing Technologies

The complete genome sequence of Streptococcus pneumoniae strain Rx1, a Hex mismatch repair-deficient standard transformation recipient, was obtained by combining Nanopore and Illumina sequencing technologies. The genome consists of a 2.03-Mb circular chromosome, with 2,054 open reading frames and a GC content of 39.72%.

scholarly journals Complete Genome Sequence of Escherichia coli Siphophage BRET

2019 ◽  
Vol 8 (5) ◽  
Author(s):  
Solange Ngazoa-Kakou ◽  
Yuyu Shao ◽  
Geneviève M. Rousseau ◽  
Audrey A. Addablah ◽  
Denise M. Tremblay ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Cote D'ivoire ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Côte D’Ivoire ◽  
Gc Content ◽  
Local Market ◽  
Cote D’Ivoire ◽  
Content Type

The lytic Escherichia coli siphophage BRET was isolated from a chicken obtained at a local market in Abidjan, Côte d’Ivoire. Its linear genome sequence consists of 59,550 bp (43.4% GC content) and contains 88 predicted genes, including 4 involved in archaeosine biosynthesis.

scholarly journals Complete Genome Sequence of Ureibacillus thermosphaericus A1, a Thermophilic Bacillus Isolated from Compost

2017 ◽  
Vol 5 (38) ◽  
Author(s):  
Hironaga Akita ◽  
Zen-ichiro Kimura ◽  
Akinori Matsushika
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
Coding Sequences ◽  
Content Type ◽  
Thermophilic Bacillus ◽  
Fukuoka Prefecture ◽  
Ureibacillus Thermosphaericus

ABSTRACT Ureibacillus thermosphaericus A1 was isolated from compost collected in Munakata City, Fukuoka Prefecture, Japan. Here, we report the first complete genome sequence of U. thermosphaericus. The complete genome of this strain consists of 3,488,104 bp with a GC content of 36.3% and comprises 3,362 predicted coding sequences.

scholarly journals Complete Genome Sequence and Methylome of the Type Strain of Shewanella algae

2021 ◽  
Vol 10 (31) ◽  
Author(s):  
Christian Tellgren-Roth ◽  
Kaisa Thorell ◽  
Michael Y. Galperin ◽  
Tino Krell ◽  
Ute Römling ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Gc Content ◽  
Single Chromosome ◽  
Content Type ◽  
Base Modification ◽  
Shewanella Algae

We report the complete genome sequence and base modification analysis of the Shewanella algae type strain CECT 5071 (= OK-1 = ATCC 51192 = DSM 9167 = IAM 14159). The genome is composed of a single chromosome of 4,924,764 bp, with a GC content of 53.10%.

scholarly journals Complete Genome Sequence of a Third- and Fourth-Generation Cephalosporin-Resistant Comamonas kerstersii Isolate

2021 ◽  
Vol 10 (28) ◽  
Author(s):  
Aline I. Moser ◽  
Edgar I. Campos-Madueno ◽  
Peter M. Keller ◽  
Andrea Endimiani
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rectal Swab ◽  
Gc Content ◽  
Generation Cephalosporin ◽  
Fourth Generation ◽  
Content Type ◽  
Class A ◽  
Lactamase Gene

Here, we report the complete genome sequence of Comamonas kerstersii 3132976, a strain isolated from a human rectal swab sample in Switzerland. The isolate was resistant to third- and fourth-generation cephalosporins and possessed a novel class A β-lactamase gene. The complete genome is 3,693,404 bp long with a GC content of 59.4%.

scholarly journals Complete Genome Sequence of Pseudomonas sp. Strain MM213, an Isolate from a Brookside in Bielefeld, Germany

2022 ◽  
Author(s):  
Bart Verwaaijen ◽  
Özgülen Cevahir ◽  
Fabian Hitz ◽  
Jacqueline Römmich ◽  
Donat Wulf
Keyword(s):  
Genome Size ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
Pseudomonas Sp ◽  
Protein Coding ◽  
Coding Sequences ◽  
Content Type ◽  
Pseudomonas Mandelii

Here, we report the complete genome sequence of Pseudomonas sp. strain MM213 of the Pseudomonas mandelii group, which was isolated from a brookside in Bielefeld, Germany. The genome size is 6,746,355 bp, with a GC content of 59.4% and 6,145 predicted protein-coding sequences.

scholarly journals Complete Genome Sequence of a Wild-Type Isolate of Caulobacter vibrioides Strain CB1

2018 ◽  
Vol 7 (12) ◽  
Author(s):  
Derrick C. Scott ◽  
Kiesha Wilson ◽  
Keshawn Ross ◽  
Damyen Ingram ◽  
Tajah Lewter ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Dna Sequences ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genome Rearrangement ◽  
Gc Content ◽  
Wild Type ◽  
Content Type ◽  
Type Isolate

The complete genome sequence of Caulobacter vibrioides strain CB1 consists of a chromosome of 4,137,285 bp, with a GC content of 67.2% and 3,990 coding DNA sequences. This strain contains the typical genome rearrangement that is characteristic of the Caulobacter strains that are currently sequenced.

scholarly journals Complete Genome Sequence of Rhodococcus erythropolis X5, a Psychrotrophic Hydrocarbon-Degrading Biosurfactant-Producing Bacterium

2019 ◽  
Vol 8 (48) ◽  
Author(s):  
Yanina Delegan ◽  
Leonid Valentovich ◽  
Kirill Petrikov ◽  
Anna Vetrova ◽  
Artur Akhremchuk ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Rhodococcus Erythropolis ◽  
Gc Content ◽  
Linear Plasmid ◽  
Circular Chromosome ◽  
Content Type ◽  
Cold Adapted ◽  
Degrading Bacterium

Rhodococcus erythropolis X5 is a psychrotrophic (cold-adapted) hydrocarbon-degrading bacterium, as it showed effective n-alkane destruction at low positive temperatures. Here, the genome of strain X5 was completely sequenced; it consists of a 6,472,161-bp circular chromosome (62.25% GC content) and a 526,979-bp linear plasmid, pRhX5-526k (62.37% GC content).

scholarly journals Complete Genome Sequence of Acidithiobacillus ferridurans JAGS, Isolated from Acidic Mine Drainage

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Jinjin Chen ◽  
Yilan Liu ◽  
Patrick Diep ◽  
Andrew Jo ◽  
Camilla Nesbø ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Mine Drainage ◽  
Gc Content ◽  
Acidic Mine Drainage ◽  
Smrt Sequencing ◽  
Protein Coding ◽  
Content Type

We report a complete genome sequence of Acidithiobacillus ferridurans JAGS, determined using PacBio single-molecule real-time (SMRT) sequencing. The circular genome of JAGS (2,933,811 bp; GC content, 58.57%) contains 3,001 protein-coding sequences, 46 tRNAs, and 6 rRNAs. Predicted genes indicate the potential to fix CO2 and N2 and to utilize Fe2+, S0, and H2 as energy sources.

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