scholarly journals Chemokine Expression Patterns Differ within Anatomically Distinct Regions of the Genital Tract during Chlamydia trachomatis Infection

2002 ◽  
Vol 70 (3) ◽  
pp. 1538-1546 ◽  
Author(s):  
Heather K. Maxion ◽  
Kathleen A. Kelly
Keyword(s):  
Genital Tract ◽  
Expression Patterns ◽  
Cd4 Cells ◽  
Chlamydia Trachomatis Infection ◽  
Chemokine Expression ◽  
Inflammatory Protein ◽  
Ascending Infection ◽  
Immune Mediated ◽  
Inducible Protein ◽  
Tubal Pathology

ABSTRACT Untreated infections with Chlamydia trachomatis commonly result in ascending infection to fallopian tubes and subsequent immune-mediated tubal pathology in females. The proposed immune-mediated injury may be associated with the increased recruitment of CD4 cells to the upper genital tract (GT) (oviducts) in comparison to the lower GT (cervix) during infection, as shown in animal models. To understand the mechanisms responsible for this biased recruitment of CD4 cells within the GT, we characterized chemokine expression patterns in the upper and lower GTs in mice during infection with the murine pneumonitis biovar of Chlamydia trachomatis. Enzyme-linked immunosorbent assays of supernatants from GT homogenates revealed that the levels of the Th1-associated chemokines CXCL9 (monokine induced by gamma interferon), CXCL10 (interferon-inducible protein 10), and CCL5 (RANTES) were significantly higher in the upper GT than in the lower GT after infection, while the CCL3 (macrophage inflammatory protein 1α) level was not increased. In contrast, the level of chemokine CCL11 (eotaxin) was significantly elevated in the lower GT later in the course of infection. Increased levels of mRNA confirmed the selective differences in chemokine expression within the upper and lower GTs. The increased levels of Th1-inducible chemokines in the upper GT were not due to differences in the magnitude of infection or progesterone pretreatment. These data demonstrate that the upper and lower regions of the GT respond differently to Chlamydia infection.

scholarly journals Chemokine Expression Patterns in the Systemic and Genital Tract Compartments are Associated with HIV-1 Infection in Women from Benin

2009 ◽  
Vol 30 (1) ◽  
pp. 90-98 ◽  
Author(s):  
Julie Lajoie ◽  
Johanne Poudrier ◽  
Marguerite Massinga Loembe ◽  
Fernand Guédou ◽  
François Leblond ◽  
...  
Keyword(s):  
Genital Tract ◽  
Expression Patterns ◽  
Chemokine Expression ◽  
Hiv 1

scholarly journals Differential Regulation of CD4 Lymphocyte Recruitment between the Upper and Lower Regions of the Genital Tract during Chlamydia trachomatis Infection

2000 ◽  
Vol 68 (3) ◽  
pp. 1519-1528 ◽  
Author(s):  
Kathleen A. Kelly ◽  
Jennifer C. Walker ◽  
Shimul H. Jameel ◽  
Heather L. Gray ◽  
Roger G. Rank
Keyword(s):  
Chlamydia Trachomatis ◽  
Genital Tract ◽  
Female Genital Tract ◽  
Chlamydia Trachomatis Infection ◽  
Flow Cytometric ◽  
Cd4 Lymphocyte ◽  
Local Recruitment ◽  
Tubal Pathology ◽  
Female Genital

ABSTRACT Genital infection with Chlamydia trachomatis results in both the local recruitment of protective immune responses and an inflammatory infiltrate that may also participate in tubal pathology. As a beginning to understanding the etiology of immune system-mediated tubal pathology, we evaluated the regional recruitment of lymphocyte subsets to different areas of the female genital tract (GT) over the course of a murine infection with the mouse pneumonitis agent ofChlamydia trachomatis (MoPn). Using flow cytometric techniques we found that the CD4 lymphocyte subset was preferentially recruited to the upper GT (oviduct and uterine horn) over the lower GT (cervical-vaginal region) throughout the course of MoPn infection. The influx of CD4 cells also correlated with the expression of endothelial cell adhesion molecules (ECAMs) and in vitro lymphocyte adherence in the upper GT. Interestingly, the expression of ECAMs in the lower GT was not maintained longer than 7 days after infection, even in the presence of viable chlamydiae. Taken together, these data suggest that regulatory mechanisms of lymphocyte recruitment differ between the upper and lower regions of the GT and may influence the clearance of chlamydiae and the development of tubal pathology.

scholarly journals Prevalence of Chlamydia trachomatis infection among women seen at the lower genital tract pathology clinic, Jundiaí School of Medicine, Brazil

2011 ◽  
Vol 9 (3) ◽  
pp. 332-336
Author(s):  
João Bosco Ramos Borges ◽  
Ana Carolina Marchesini ◽  
Luciana Francine Bocchi De Stefani ◽  
Marcus Vinícius Gonçalves Belintani ◽  
Thaís Andrea dos Santos
Keyword(s):  
Chlamydia Trachomatis ◽  
Human Papilloma Virus ◽  
Genital Tract ◽  
Human Papilloma Virus Infection ◽  
Papilloma Virus ◽  
Chlamydia Trachomatis Infection ◽  
Screening Programs ◽  
Lower Genital Tract ◽  
Number Of Patients ◽  
The City

ABSTRACT Objective: To estimate the prevalence of Chlamydia trachomatis in a population with a high risk of sexually transmitted diseases and to compare data of the literature and the relationship of infection with the presence of human papilloma virus induced lesions. Methods: A total of 28 hybrid capture tests for C. trachomatis were collected from patients referred to the Municipal Health Division of the city of Jundiaí (SP) for the lower genital tract pathology. The results were compared with findings in the literature, and with the test results from a general population of the city of Jundiaí. Results: Of the 28 tests, 3 (10.7%) were positive. We did not find a positive association between C. trachomatis infection and the presence or aggravation of intraepithelial cervical cancer. Conclusion: Our findings showed a high prevalence of C. trachomatis infection in the population studied, but no association with human papilloma virus infection. Because the number of patients assessed was small, it is difficult to generalize from our findings. We suggest there is a need to expand screening programs for C. trachomatis, mainly in symptomatic patients and in those patients with cervical changes.

Defensin and chemokine expression patterns in the palatine tonsil: a model of their local interaction

2005 ◽  
Vol 263 (4) ◽  
pp. 319-326 ◽  
Author(s):  
Jens Eduard Meyer ◽  
Ulf Henning Beier ◽  
Tibor Görögh ◽  
Sebastian Schreiber ◽  
Christian Beck ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Local Interaction ◽  
Palatine Tonsil ◽  
Chemokine Expression

scholarly journals Differential chemokine expression patterns in tonsillar disease

2018 ◽  
Vol 38 (4) ◽  
pp. 316-322
Author(s):  
M. Mandapathil ◽  
U. H. Beier ◽  
H. Graefe ◽  
B. Kröger ◽  
J. Hedderich ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Chemokine Expression

scholarly journals Campylobacter jejuni Induces Secretion of Proinflammatory Chemokines from Human Intestinal Epithelial Cells

2005 ◽  
Vol 73 (7) ◽  
pp. 4437-4440 ◽  
Author(s):  
Lan Hu ◽  
Thomas E. Hickey
Keyword(s):  
Campylobacter Jejuni ◽  
Inflammatory Responses ◽  
Intestinal Epithelial ◽  
Mrna Transcription ◽  
Contributing Factors ◽  
Monocyte Chemoattractant Protein 1 ◽  
Inflammatory Protein ◽  
Human Intestinal Epithelial Cells ◽  
Inducible Protein ◽  
Immunological Assays

ABSTRACT Campylobacter jejuni is a common cause of diarrhea in humans. While the pathogenic mechanisms of C. jejuni are not completely understood, host inflammatory responses are thought to be contributing factors. In this report, C. jejuni 81-176 is shown to up-regulate chemokines essential to inflammatory responses. Growth-related oncogene α (GROα), GROγ, macrophage inflammatory protein 1, monocyte chemoattractant protein 1 (MCP-1), and gamma interferon-inducible protein 10 (γIP-10) mRNA transcription in INT-407 cells was enhanced within 4 h of bacterial exposure. Infection with viable campylobacters was necessary for sustained chemokine transcription and was NF-κB dependent. GROα, γIP-10, and MCP-1 chemokine secretions were confirmed by immunological assays.

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