scholarly journals Identification of a Novel Cathelicidin Gene in the Rainbow Trout, Oncorhynchus mykiss

2005 ◽  
Vol 73 (8) ◽  
pp. 5053-5064 ◽  
Author(s):  
Chin-I Chang ◽  
Olga Pleguezuelos ◽  
Yong-An Zhang ◽  
Jun Zou ◽  
Christopher J. Secombes
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Antimicrobial Peptide ◽  
Primary Cultures ◽  
Head Kidney ◽  
Cationic Antimicrobial Peptide ◽  
Specific Amino Acid ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Peptide Gene ◽  
Core Part

ABSTRACT We report the cloning of a novel antimicrobial peptide gene, termed rtCATH_1, found in the rainbow trout, Oncorhynchus mykiss. The predicted 216-residue rtCATH_1 prepropeptide consists of three domains: a 22-residue signal peptide, a 128-residue cathelin-like region containing two identifiable cathelicidin family signatures, and a predicted 66-residue C-terminal cationic antimicrobial peptide. This predicted mature peptide was unique in possessing features of different known (mammalian) cathelicidin subgroups, such as the cysteine-bridged family and the specific amino-acid-rich family. The rtCATH_1 gene comprises four exons, as seen in all known mammalian cathelicidin genes, and several transcription factor binding sites known to be of relevance to host defenses were identified in the 5′ flanking region. By Northern blot analysis, the expression of rtCATH_1 was detected in gill, head kidney, and spleen of bacterially challenged fish. Primary cultures of head kidney leukocytes from rainbow trout stimulated with lipopolysaccharide or poly(I · C) also expressed rtCATH_1. A 36-residue peptide corresponding to the core part of the fish cathelicidin was chemically synthesized and shown to exhibit potent antimicrobial activity and a low hemolytic effect. Thus, rtCATH_1 represents a novel antimicrobial peptide gene belonging to the cathelicidin family and may play an important role in the innate immunity of rainbow trout.

The effect of peptidoglycan enriched diets on antimicrobial peptide gene expression in rainbow trout (Oncorhynchus mykiss)

2013 ◽  
Vol 34 (2) ◽  
pp. 529-537 ◽  
Author(s):  
Elisa Casadei ◽  
Steve Bird ◽  
Jose L. González Vecino ◽  
Simon Wadsworth ◽  
Christopher J. Secombes
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Antimicrobial Peptide ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Peptide Gene

scholarly journals Optimisation of the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss)

2019 ◽  
Vol 64 (No. 12) ◽  
pp. 547-557
Author(s):  
H Minarova ◽  
M Palikova ◽  
J Mares ◽  
E Syrova ◽  
J Blahova ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Bovine Serum ◽  
Lymphocyte Proliferation ◽  
Head Kidney ◽  
Pokeweed Mitogen ◽  
Lymphocyte Proliferation Assay ◽  
Proliferation Assay ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Foetal Bovine Serum

The lymphocyte proliferation assay is a valuable method used for the evaluation of the fish immune system. However, there are many variations and optimal results are not always obtained. Unification is necessary to ensure the comparability between different studies. The aim of this study was to optimise the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss). This goal included the determination of the optimal incubation length, serum type, incubation temperature, type of mitogen and its concentration, and anticoagulant. The peripheral blood and head kidney lymphocytes were isolated by density gradient centrifugation. Subsequently, the cells were incubated for 3–8 days with different mitogens (pokeweed mitogen 5, 10 and 50 µg/ml, concanavalin A 1, 10 and 20 µg/ml, phytohaemagglutinin 25, 50 and 100 µg/ml, lipopolysaccharide 1, 50 and 100 µg/ml). The use of the different serum types (foetal bovine serum, trout serum), incubation temperatures (10–20 °C) and anticoagulants (heparin, EDTA) was compared. Labelled thymidine was used to evaluate the assay. The best results were obtained after seven days of incubation at 15 °C with foetal bovine serum (FBS). The head kidney lymphocytes showed the highest proliferative response with 50 µg/ml phytohaemagglutinin. With the peripheral blood lymphocytes (heparin and EDTA), the best results were obtained with 50 µg/ml pokeweed mitogen. The highest proliferation levels were detected with heparinised blood. In conclusion, optimisation of this assay contributes to the improved assessment of the rainbow trout immune function.

scholarly journals Development of a monoclonal antibody for ELISA of CYP1A in primary cultures of rainbow trout Oncorhynchus mykiss hepatocytes

Biomarkers ◽  
1997 ◽  
Vol 2 (5) ◽  
pp. 287-294 ◽  
Author(s):  
S. Scholz ◽  
I. Behn ◽  
H. Honeck ◽  
C. Hauck ◽  
T. Braunbeck ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Primary Cultures ◽  
Rainbow Trout Oncorhynchus Mykiss

Transcriptomic responses to heat stress in rainbow trout Oncorhynchus mykiss head kidney

2018 ◽  
Vol 82 ◽  
pp. 32-40 ◽  
Author(s):  
Jinqiang Huang ◽  
Yongjuan Li ◽  
Zhe Liu ◽  
Yujun Kang ◽  
Jianfu Wang
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Head Kidney ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Transcriptomic Responses

An in vitro examination of selenium–cadmium antagonism using primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes

Metallomics ◽  
2016 ◽  
Vol 8 (2) ◽  
pp. 218-227 ◽  
Author(s):  
Ankur Jamwal ◽  
Mohammad Naderi ◽  
Som Niyogi
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Primary Cultures ◽  
Ros Generation ◽  
Antagonistic Effects ◽  
Low Concentration ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Dose Dependent ◽  
In Vitro Examination

Se has antagonistic effects on Cd-induced cytotoxicityviaboth enzymatic and non-enzymatic antioxidative mechanisms and the effects are strictly dose dependent. Confocal fluorescent images of isolated rainbow trout hepatocytes exposed to 100 µM Cd, alone or in combination with low (25 µM) or high (250 µM) concentration of Se, show reduced ROS generation with low concentration of Se.

scholarly journals 11-Deoxycorticosterone Is a Potent Agonist of the Rainbow Trout (Oncorhynchus mykiss) Mineralocorticoid Receptor

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 47-55 ◽  
Author(s):  
A. Sturm ◽  
N. Bury ◽  
L. Dengreville ◽  
J. Fagart ◽  
G. Flouriot ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Mineralocorticoid Receptor ◽  
Head Kidney ◽  
Luciferase Reporter ◽  
Similar Response ◽  
Hormonal Stimulation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Juvenile Trout

The teleost fish are thought to lack the mineralocorticoid hormone aldosterone but possess mineralocorticoid receptor (MR) homologs. Here we describe the characterization of two rainbow trout (Oncorhynchus mykiss) MRs, called rtMRa and rtMRb. The open reading frame of rtMRa cDNA encoded a protein of 1041 amino acids. The rtMRb predicted protein sequence is similar, differing in only 10 amino acids in the nonconserved A/B domain and lacking a three-amino acid insertion between the two zinc fingers of the C domain. Expression of rtMR mRNA (sum of both forms), measured in juvenile trout by real-time RT-PCR, shows that the transcripts are ubiquitous. Expression was significantly higher in brain than the other tissues studied (eye, trunk kidney, head kidney, gut, gills, liver, spleen, ovary, heart, white muscle, skin). Hormonal stimulation of receptor transactivation activity was studied in COS-7 cells transiently cotransfected with receptor cDNA and a mouse mammary tumor virus-luciferase reporter. The mineralocorticoids 11-deoxycorticosterone and aldosterone were more potent enhancers of rtMRa transcriptional activity (EC50 = 1.6 ± 0.5 × 10−10 and 1.1 ± 0.4 × 10−10m, respectively) than the glucocorticoids cortisol and 11-deoxycortisol (EC50 = 1.1 ± 0.3 × 10−9 and 3.7 ± 1.9 × 10−9m, respectively). A similar response was observed in transactivation assays with rtMRb. These results are discussed in the view of reported circulating levels of corticosteroids in trout.

scholarly journals Draft Genome Sequence of Epilithonimonas sp. FP211-J200, Isolated from an Outbreak Episode on a Rainbow Trout (Oncorhynchus mykiss) Farm

2017 ◽  
Vol 5 (37) ◽  
Author(s):  
Manuel Ayala ◽  
Cristopher Segovia ◽  
Rodrigo Rojas ◽  
Claudio Miranda ◽  
Javier Santander
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Genome Sequence ◽  
Draft Genome ◽  
Head Kidney ◽  
Fish Host ◽  
Draft Genome Sequence ◽  
Kidney Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Head Kidney Cells

ABSTRACT Here, we report the draft genome sequence of Epilithonimonas sp. FP211-J200, isolated from rainbow trout head kidney cells. The size of the genome is 4,110,772 bp, with a G+C content of 37.1%. The Epilithonimonas sp. FP211-J200 genome has genes related to tetracycline and β-lactam resistance. This is the first reported Epilithonimonas species genome isolated from a fish host.

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