scholarly journals Timing of swarmer cell cycle morphogenesis and macromolecular synthesis by Hyphomicrobium neptunium in synchronous culture.

1980 ◽  
Vol 144 (1) ◽  
pp. 406-412 ◽  
Author(s):  
T M Wali ◽  
G R Hudson ◽  
D A Danald ◽  
R M Weiner
Keyword(s):  
Cell Cycle ◽  
Synchronous Culture ◽  
Macromolecular Synthesis ◽  
Swarmer Cell

Oxygen uptake during the cell cycle of the fission yeast Schizosaccharomyces pombe

1978 ◽  
Vol 33 (1) ◽  
pp. 399-411
Author(s):  
J. Creanor
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Oxygen Uptake ◽  
Dna Synthesis ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Nuclear Division ◽  
Synchronous Culture ◽  
Synchronous Cultures ◽  
The Many

Oxygen uptake was measured in synchronous cultures of the fission yeast Schizosaccharomyces pombe. The rate of oxygen uptake was found to increase in a step-wise manner at the beginning of the cycle and again in the middle of the cycle. The increases in rate were such that overall, oxygen uptake doubled in rate once per cell cycle. Addition of inhibitors of DNA synthesis or nuclear division to a synchronous culture did not affect the uptake of oxygen. In an induced synchronous culture, in which DNA synthesis, cell division, and nuclear division, but not ‘growth’ were synchronized, oxygen uptake increased continuously in rate and did not show the step-wise rises which were shown in the selection-synchronized culture. These results were compared with previous measurements of oxygen uptake in yeast and an explanation is suggested for the many different patterns which have been reported.

Changes in the Wall-Bound Glycosidase Activities during the Cell Cycle in a Synchronous Culture of Catharanthus roseus

1989 ◽  
Vol 44 (9-10) ◽  
pp. 754-756 ◽  
Author(s):  
Shin-ichi Amino ◽  
Atsushi Komamine
Keyword(s):  
Cell Cycle ◽  
Catharanthus Roseus ◽  
Cell Walls ◽  
Synchronous Culture ◽  
Galactosidase Activity

The activities of some of the glycosidases in cell walls were measured during the cell cycle in a synchronous culture of Catharanthus roseus (L.) G. Don. The salt-extractable α-galactosidase activity increased during the G 2 phase, whereas β-glucosidase and β-galactosidase activities in cell walls increased during the G 1 phase after cytokinesis.

scholarly journals Flow cytometric analysis of the cell cycle in synchronous culture of Catharanthus roseus.

1987 ◽  
Vol 51 (5) ◽  
pp. 1443-1445 ◽  
Author(s):  
Satoshi ANDO ◽  
Toshio SHIMIZU ◽  
Hiroaki KODAMA ◽  
Shin-ichi AMINO ◽  
Atsushi KOMAMINE
Keyword(s):  
Cell Cycle ◽  
Catharanthus Roseus ◽  
Flow Cytometric Analysis ◽  
Synchronous Culture ◽  
Flow Cytometric

scholarly journals Synchronous Culture and the Cell Cycle in Plants

1986 ◽  
Vol 3 (1) ◽  
pp. 9-15
Author(s):  
Shin-ichi AMINO ◽  
Sakiko AKADA ◽  
Hiroaki KODAMA ◽  
Atsushi KOMAMINE
Keyword(s):  
Cell Cycle ◽  
Synchronous Culture

An autoradiographical study of amino acid and nucleoside incorporation during the cell cycle of Amoeba proteus

1981 ◽  
Vol 51 (1) ◽  
pp. 219-228
Author(s):  
K.I. Mills ◽  
L.G. Bell
Keyword(s):  
Cell Cycle ◽  
Amino Acids ◽  
Amino Acid ◽  
Rna Synthesis ◽  
Thymidine Incorporation ◽  
Tritiated Thymidine ◽  
Amoeba Proteus ◽  
Macromolecular Synthesis ◽  
Future Studies ◽  
Specific Proteins

The incorporation of tritiated thymidine, uridine and leucine, into the acid-precipitable material of DNA. RNA and proteins, respectively, was studied by autoradiography throughout the cell cycle of Amoeba proteus. DNA synthesis occupied the first 17 h of the cycle (57 h long) and 2 peaks between 0.5 and 9.13 h accounted for the majority of the thymidine incorporation. RNA synthesis was represented by a series of peak uridine grain counts, the 3 major peaks occurring at 10, 26–27 and 47–48 h. The incorporation of leucine also followed a pattern of peaks and dips, the main peaks occurring 1–2 h after the major increases in uridine incorporation. The fraction of label present over the nucleus decreased during the cell cycle, and this was probably due to a lowered incorporation of the leucine label by proteins synthesized in the cytoplasm and destined to become nuclear proteins. The incorporation patterns of 6 amino acids (arginine, aspartic acid, leucine, lysine, serine and valine) were studied individually during 3 periods of the cell cycle: 0-10 h (S phase); 20–30 h (early G2); and 40–50 h (mid-late G2). Variations in the intensity and timings of the incorporation maxima of the amino acids were observed, although the timings of increased grain counts of some of the amino acids frequently coincided. “Unique” incorporation peaks (i.e. only observed in one of the amino acids studied) possibly indicate the synthesis of phase-specific proteins. The amino acid and nucleoside incorporation profiles presented in this paper will enable the results obtained from future studies on amoebae to be related to the macromolecular synthesis patterns.

Rates of synthesis of polyadenylated messenger RNA and ribosomal RNA during the cell cycle of Schizosaccharomyces pombe. With an appendix: calculation of the pattern of protein accumulation from observed changes in the rate of messenger RNA synthesis

1976 ◽  
Vol 21 (3) ◽  
pp. 497-521
Author(s):  
R.S. Fraser ◽  
F. Moreno
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Schizosaccharomyces Pombe ◽  
Ribosomal Rna ◽  
Messenger Rna ◽  
Rna Synthesis ◽  
Protein Accumulation ◽  
Synchronous Culture ◽  
Synchronous Cultures ◽  
Gene Copies

The rates of polyadenylated messenger RNA and ribosomal RNA synthesis were measured in synchronously dividing cultures of fission yeast (Schizosaccharomyces pombe). Control asynchronous cultures, which had been exposed to the conditions used for preparing synchronous cultures, were investigated to check for effects of the synchronization procedure itself on RNA synthesis. After each period of DNA synthesis in synchronous culture, the rates of messenger and ribosomal RNA synthesis doubled, suggesting that gene number controls the rate of messenger and ribosomal RNA synthesis. This was confirmed by experiments with asynchronous, exponential-phase cultures in which DNA synthesis was inhibited by hydroxyurea. Both synchronous culture and hydroxyurea experiments suggested that there is a delay of 15 min (0-1 of the cell generation time) between replication of the DNA and transcription of both gene copies. A pattern of protein accumulation was calculated from changes in the rate of polyadenylated messenger RNA synthesis during synchronous culture. The simulated pattern indicates that protein is accumulated linearly, with a doubling in the rate of accumulation once per cell cycle. The simulated pattern of protein accumulation is very similar to measurements previously reported by other workers of changes in activities of 3 enzymes in synchronous cultures. It is suggested that the doubling of the rate of messenger RNA synthesis, as a consequence of the replication of the DNA once per cycle, provides the basis of a mechanism for control of the doubling of other cellular constituents during the cell cycle.

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