scholarly journals 4,4′-Diapophytoene Desaturase: Catalytic Properties of an Enzyme from the C30 Carotenoid Pathway ofStaphylococcus aureus

1999 ◽  
Vol 181 (19) ◽  
pp. 6184-6187 ◽  
Author(s):  
Axel Raisig ◽  
Gerhard Sandmann
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Substrate Specificity ◽  
Affinity Chromatography ◽  
Catalytic Properties ◽  
Ofstaphylococcus Aureus ◽  
Double Bonds ◽  
One Step ◽  
Single Enzyme ◽  
Carotenoid Pathway

ABSTRACT Staphylococcus aureus synthesizes C30carotenoids. Their formation involves the introduction of three double bonds, which is catalyzed by a single enzyme. This enzyme, 4,4′-diapophytoene desaturase from S. aureus, was overexpressed in Escherichia coli and purified in one step by affinity chromatography, and then the protein was characterized with respect to substrate specificity, cofactor requirement, and oligomerization.

scholarly journals Escherichia coli RNase D. Catalytic properties and substrate specificity.

1981 ◽  
Vol 256 (11) ◽  
pp. 5633-5637 ◽  
Author(s):  
H. Cudny ◽  
R. Zaniewski ◽  
M.P. Deutscher
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Catalytic Properties

One-Step Synthesis and Antibacterial Properties of Polyaniline/TiO2 Nanocomposites

2012 ◽  
Vol 534 ◽  
pp. 78-81
Author(s):  
Ting Wei Hu ◽  
Shao Yun Shan ◽  
Shi Mei Yang ◽  
Li Hong Jiang ◽  
Ya Ming Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Interfacial Polymerization ◽  
Antibacterial Property ◽  
Antibacterial Properties ◽  
Molar Ratio ◽  
Tetrabutyl Titanate ◽  
One Step ◽  
Polymerization Method

Polyaniline(PANI)/TiO2 nanocomposites are successful synthesized by one step-interfacial polymerization method. Morphologies and structures of the nanocomposites are characterized using SEM, FTIR, and XRD. The antibacterial properties of PANI/TiO2 nanocomposites on Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Bacillus megateriu and Bacillus cereus are tested. The effect of molar ratio of aniline and tetrabutyl titanate on structures and antibacterial properties are also been explored. The results show that the content of tetrabutyl titanate has a obvious influence on structure and morphologies of the nanocomposites. PANI/TiO2 nanocomposites have antibacterial properties especially on Staphylococcus aureus and Escherichia coli, which shows a best antibacterial property when the molar ratio of aniline to tetrabutyl titanate is 3:2.

scholarly journals Evolution of the C30 Carotenoid Synthase CrtM for Function in a C40 Pathway

2002 ◽  
Vol 184 (23) ◽  
pp. 6690-6699 ◽  
Author(s):  
Daisuke Umeno ◽  
Alexander V. Tobias ◽  
Frances H. Arnold
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Substrate Specificity ◽  
Significant Variation ◽  
The Other ◽  
Biosynthetic Pathways ◽  
Product Range

ABSTRACT The C30 carotene synthase CrtM from Staphylococcus aureus and the C40 carotene synthase CrtB from Erwinia uredovora were swapped into their respective foreign C40 and C30 biosynthetic pathways (heterologously expressed in Escherichia coli) and evaluated for function. Each displayed negligible ability to synthesize the natural carotenoid product of the other. After one round of mutagenesis and screening, we isolated 116 variants of CrtM able to synthesize C40 carotenoids. In contrast, we failed to find a single variant of CrtB with detectable C30 activity. Subsequent analysis revealed that the best CrtM mutants performed comparably to CrtB in an in vivo C40 pathway. These mutants showed significant variation in performance in their original C30 pathway, indicating the emergence of enzymes with broadened substrate specificity as well as those with shifted specificity. We discovered that Phe 26 alone determines the specificity of CrtM. The plasticity of CrtM with respect to its substrate and product range highlights the potential for creating further new carotenoid backbone structures.

scholarly journals One-step purification of the NADH dehydrogenase fragment of the Escherichia coli complex I by means of Strep -tag affinity chromatography

FEBS Letters ◽  
1999 ◽  
Vol 460 (2) ◽  
pp. 207-211 ◽  
Author(s):  
Stefanie Bungert ◽  
Bianca Krafft ◽  
Ramona Schlesinger ◽  
Thorsten Friedrich
Keyword(s):  
Escherichia Coli ◽  
Affinity Chromatography ◽  
Complex I ◽  
Nadh Dehydrogenase ◽  
One Step

scholarly journals Functional Reconstitution of SdcS, a Na+-Coupled Dicarboxylate Carrier Protein from Staphylococcus aureus

2006 ◽  
Vol 189 (3) ◽  
pp. 880-885 ◽  
Author(s):  
Jason A. Hall ◽  
Ana M. Pajor
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Substrate Specificity ◽  
Transport Properties ◽  
Family Member ◽  
Partial Purification ◽  
Carrier Protein ◽  
Electrochemical Gradient ◽  
Structural Aspects

ABSTRACT In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expressed SdcS in Escherichia coli and found it to have transport properties similar to those of its eukaryotic counterparts (J. A. Hall and A. M. Pajor, J. Bacteriol. 187:5189-5194, 2005). Here, we report the partial purification and subsequent reconstitution of functional SdcS into liposomes. These proteoliposomes exhibited succinate counterflow activity, as well as Na+ electrochemical-gradient-driven transport. Examination of substrate specificity indicated that the minimal requirement necessary for transport was a four-carbon terminal dicarboxylate backbone and that productive substrate-transporter interaction was sensitive to substitutions at the substrate C-2 and C-3 positions. Further analysis established that SdcS facilitates an electroneutral symport reaction having a 2:1 cation/dicarboxylate ratio. This study represents the first characterization of a reconstituted Na+-coupled DASS family member, thus providing an effective method to evaluate functional, as well as structural, aspects of DASS transporters in a system free of the complexities and constraints associated with native membrane environments.

scholarly journals Ribotyping ofStaphylococcus aureus: an assessment using well–defined strains

1994 ◽  
Vol 112 (1) ◽  
pp. 93-101 ◽  
Author(s):  
J. F. Richardson ◽  
P. Aparicio ◽  
R. R. Marples ◽  
B. D. Cookson
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Phage Type ◽  
Ofstaphylococcus Aureus ◽  
Human Origin ◽  
Primary Method ◽  
Epidemiological Typing ◽  
Typing Method ◽  
Phage Group ◽  
Group Iii

SummaryRibotyping, with homologous or heterologous (Escherichia coli) r–RNA, of the propagating strains for phages of the international set for strains ofStaphylococcus aureusof human origin was undertaken to determine the discrimination of this typing method. Ribotyping could distinguish between strains of different phage groups, but could not distinguish between seven phage group III strains of different phage type. Ribotyping may be a useful adjunct to phage typing inS. aureusbut is unlikely to replace it as the primary method of epidemiological typing.

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