Megaplasmid pRme2011a of Sinorhizobium meliloti Is Not Required for Viability
2000 ◽
Vol 182
(12)
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pp. 3582-3586
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Keyword(s):
ABSTRACT We report the curing of the 1,360-kb megaplasmid pRme2011a fromSinorhizobium meliloti strain Rm2011. With a positive selection strategy that utilized Tn5B12-S containing thesacB gene, we were able to cure this replicon by successive rounds of selecting for deletion formation in vivo. Subsequent Southern blot, Eckhardt gel, and pulsed-field gel electrophoresis analyses were consistent with the hypothesis that the resultant strain was indeed missing pRme2011a. The cured derivative grew as well as the wild-type strain in both complex and defined media but was unable to use a number of substrates as a sole source of carbon on defined media.
2013 ◽
Vol 58
(3)
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pp. 1671-1677
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Keyword(s):
2011 ◽
Vol 77
(17)
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pp. 6020-6026
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Keyword(s):
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1971 ◽
Vol 29
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pp. 430-431
Keyword(s):
1991 ◽
Vol 29
(9)
◽
pp. 1780-1784
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2000 ◽
Vol 182
(3)
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pp. 599-606
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