scholarly journals Distribution of Twelve Linear Extrachromosomal DNAs in Natural Isolates of Lyme Disease Spirochetes

2000 ◽  
Vol 182 (9) ◽  
pp. 2476-2480 ◽  
Author(s):  
Nanette Palmer ◽  
Claire Fraser ◽  
Sherwood Casjens
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Type Strain ◽  
Sensu Stricto ◽  
Linear Plasmids ◽  
Borrelia Garinii ◽  
Natural Isolates ◽  
Disease Agent ◽  
Lyme Disease Agent ◽  
Sequence Similarities

ABSTRACT We have analyzed a panel of independent North American isolates of the Lyme disease agent spirochete, Borrelia burgdorferi (sensu stricto), for the presence of linear plasmids with sequence similarities to the 12 linear plasmids present in theB. burgdorferi type strain, isolate B31. The frequency of similarities to probes from each of the 12 B31 plasmids varied from 13 to 100% in the strain panel examined, and these similarities usually reside on plasmids similar in size to the cognate B31 plasmid. Sequences similar to 5 of the 12 B31 plasmids were found in all of the isolates examined, and >66% of the panel members hybridized to probes from 4 other plasmids. Sequences similar to most of the B. burgdorferi B31 plasmid-derived DNA probes used were also found on linear plasmids in the related Eurasian Lyme agentsBorrelia garinii and Borrelia afzelii; however, some of these plasmids had uniform but substantially different sizes from their B. burgdorferi counterparts.

scholarly journals DNA Microarray Assessment of Putative Borrelia burgdorferi Lipoprotein Genes

2002 ◽  
Vol 70 (6) ◽  
pp. 3300-3303 ◽  
Author(s):  
Fang Ting Liang ◽  
F. Kenneth Nelson ◽  
Erol Fikrig
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Dna Microarray ◽  
Sensu Stricto ◽  
Borrelia Garinii ◽  
Borrelia Afzelii ◽  
Genes Encoding ◽  
The Individual

ABSTRACT A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.

scholarly journals Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Karie E. Robertson ◽  
Chloe D. Truong ◽  
Felicia M. Craciunescu ◽  
Jay-How Yang ◽  
Po-Lin Chiu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Virulence Factors ◽  
Protein Complexes ◽  
Signal Sequence ◽  
Surface Proteins ◽  
Disease Agent ◽  
And Function ◽  
Lyme Disease Agent

AbstractMembrane-embedded proteins are critical to the establishment, survival and persistence in the host of the Lyme disease bacterium Borrelia burgdorferi (Bb), but to date, there are no solved structures of transmembrane proteins representing these attractive therapeutic targets. All available structures from the genus Borrelia represent proteins expressed without a membrane-targeting signal peptide, thus avoiding conserved pathways that modify, fold and assemble membrane protein complexes. Towards elucidating structure and function of these critical proteins, we directed translocation of eleven expression-optimized Bb virulence factors, including the signal sequence, to the Escherichia coli membrane, of which five, BBA57, HtrA, BB0238, BB0323, and DipA, were expressed with C-terminal His-tags. P66 was also expressed using the PelB signal sequence fused to maltose binding protein. Membrane-associated BBA57 lipoprotein was solubilized by non-ionic and zwitterionic detergents. We show BBA57 translocation to the outer membrane, purification at a level sufficient for structural studies, and evidence for an α-helical multimer. Previous studies showed multiple critical roles of BBA57 in transmission, joint arthritis, carditis, weakening immune responses, and regulating other Bb outer surface proteins. In describing the first purification of membrane-translocated BBA57, this work will support subsequent studies that reveal the precise mechanisms of this important Lyme disease virulence factor.

scholarly journals Plasmid diversity and phylogenetic consistency in the Lyme disease agent Borrelia burgdorferi

BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Sherwood R. Casjens ◽  
Eddie B. Gilcrease ◽  
Marija Vujadinovic ◽  
Emmanuel F. Mongodin ◽  
Benjamin J. Luft ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Disease Agent ◽  
Lyme Disease Agent ◽  
Plasmid Diversity

scholarly journals Amblyomma americanum (Acari: Ixodidae) Ticks Are Not Vectors of the Lyme Disease Agent, Borrelia burgdorferi (Spirocheatales: Spirochaetaceae): A Review of the Evidence

2018 ◽  
Vol 55 (3) ◽  
pp. 501-514 ◽  
Author(s):  
Ellen Y Stromdahl ◽  
Robyn M Nadolny ◽  
Graham J Hickling ◽  
Sarah A Hamer ◽  
Nicholas H Ogden ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Amblyomma Americanum ◽  
Disease Agent ◽  
Lyme Disease Agent

scholarly journals Conservation of Plasmid Maintenance Functions between Linear and Circular Plasmids in Borrelia burgdorferi

2003 ◽  
Vol 185 (10) ◽  
pp. 3202-3209 ◽  
Author(s):  
Philip E. Stewart ◽  
George Chaconas ◽  
Patricia Rosa
Keyword(s):  
Borrelia Burgdorferi ◽  
Linear Plasmid ◽  
Linear Plasmids ◽  
Autonomous Replication ◽  
Plasmid Maintenance ◽  
Disease Agent ◽  
Mammalian Infection ◽  
Lyme Disease Agent ◽  
Paralogous Gene Family ◽  
Derivatives Of

ABSTRACT The Lyme disease agent Borrelia burgdorferi maintains both linear and circular plasmids that appear to be essential for mammalian infection. Recent studies have characterized the circular plasmid regions that confer autonomous replication, but the genetic elements necessary for linear plasmid maintenance have not been experimentally identified. Two vectors derived from linear plasmids lp25 and lp28-1 were constructed and shown to replicate autonomously in B. burgdorferi. These vectors identify internal regions of linear plasmids necessary for autonomous replication in B. burgdorferi. Although derived from linear plasmids, the vectors are maintained in circular form in B. burgdorferi, indicating that plasmid maintenance functions are conserved, regardless of DNA form. Finally, derivatives of these vectors indicate that paralogous gene family 49 is apparently not required for either circular or linear plasmid replication.

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