Characterization of a Nucleotide-Binding Domain Associated with Neisserial Iron Transport
2004 ◽
Vol 186
(10)
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pp. 3266-3269
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Keyword(s):
ABSTRACT The fbpABC operon in Neisseria gonorrhoeae encodes an ATP-binding cassette transporter required for iron uptake from the host ferric binding proteins. The gene for the nucleotide-binding domain (fbpC) expressed in Escherichia coli has intrinsic ATPase activity (0.5 mmol/min/mg) uncoupled from the iron transport process. The FbpC E164D mutant is found to have a 10-fold reduction in specific activity. FbpC is covalently modified by 8-azido-[γ32P]ATP, indicating that FbpC is a functional ATPase that likely combines with FbpB to form a ferric iron transporter.
2020 ◽
Vol 16
(9)
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pp. 1507-1525
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1989 ◽
Vol 160
(1)
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pp. 25-32
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Keyword(s):
1997 ◽
Vol 7
(16)
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pp. 2109-2114
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Keyword(s):
1995 ◽
Vol 270
(23)
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pp. 14085-14093
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2012 ◽
Vol 287
(28)
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pp. 23923-23931
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2003 ◽
Vol 278
(29)
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pp. 26862-26869
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2011 ◽
Vol 405
(1)
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pp. 19-23
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Keyword(s):