scholarly journals SOS-Independent Induction of dinB Transcription by β-Lactam-Mediated Inhibition of Cell Wall Synthesis in Escherichia coli

2005 ◽  
Vol 187 (4) ◽  
pp. 1515-1518 ◽  
Author(s):  
Tatiana Pérez-Capilla ◽  
María-Rosario Baquero ◽  
José-María Gómez-Gómez ◽  
Alina Ionel ◽  
Soledad Martín ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Dna Polymerase ◽  
Frameshift Mutation ◽  
Regulatory System ◽  
Cell Wall Synthesis ◽  
Gene Encoding ◽  
Lactam Antibiotic ◽  
Dna Polymerase Iv ◽  
Different Levels

ABSTRACT Transcription of the dinB gene, encoding DNA polymerase IV, is induced by the inhibition of cell wall synthesis at different levels. Using the β-lactam antibiotic ceftazidime, a PBP3 inhibitor, as a model, we have shown that this induction is independent of the LexA/RecA regulatory system. Induction of dinB transcription mediated by ceftazidime produces an increase in the reversion of a +1 Lac frameshift mutation.

scholarly journals Interactions between Penicillin-Binding Proteins (PBPs) and Two Novel Classes of PBP Inhibitors, Arylalkylidene Rhodanines and Arylalkylidene Iminothiazolidin-4-ones

2004 ◽  
Vol 48 (3) ◽  
pp. 961-969 ◽  
Author(s):  
Astrid Zervosen ◽  
Wei-Ping Lu ◽  
Zhouliang Chen ◽  
Ronald E. White ◽  
Thomas P. Demuth ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Cell Wall Synthesis ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Penicillin Binding Proteins ◽  
E Coli ◽  
Peptidoglycan Biosynthesis ◽  
Vancomycin Resistant ◽  
Inhibitory Activities

ABSTRACT Several non-β-lactam compounds were active against various gram-positive and gram-negative bacterial strains. The MICs of arylalkylidene rhodanines and arylalkylidene iminothiazolidin-4-ones were lower than those of ampicillin and cefotaxime for methicillin-resistant Staphylococcus aureus MI339 and vancomycin-resistant Enterococcus faecium EF12. Several compounds were found to inhibit the cell wall synthesis of S. aureus and the last two steps of peptidoglycan biosynthesis catalyzed by ether-treated cells of Escherichia coli or cell wall membrane preparations of Bacillus megaterium. The effects of the arylalkylidene rhodanines and arylalkylidene iminothiazolidin-4-one derivatives on E. coli PBP 3 and PBP 5, Streptococcus pneumoniae PBP 2xS (PBP 2x from a penicillin-sensitive strain) and PBP 2xR (PBP 2x from a penicillin-resistant strain), low-affinity PBP 2a of S. aureus, and the Actinomadura sp. strain R39 and Streptomyces sp. strain R61 dd-peptidases were studied. Some of the compounds exhibited inhibitory activities in the 10 to 100 μM concentration range. The inhibition of PBP 2xS by several of them appeared to be noncompetitive. The dissociation constant for the best inhibitor (Ki = 10 μM) was not influenced by the presence of the substrate.

scholarly journals Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamily

1999 ◽  
Vol 96 (21) ◽  
pp. 11922-11927 ◽  
Author(s):  
V. L. Gerlach ◽  
L. Aravind ◽  
G. Gotway ◽  
R. A. Schultz ◽  
E. V. Koonin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Polymerase Iv ◽  
Human And Mouse

scholarly journals A strategically located serine residue is critical for the mutator activity of DNA polymerase IV from Escherichia coli

2013 ◽  
Vol 41 (9) ◽  
pp. 5104-5114 ◽  
Author(s):  
Amit Sharma ◽  
Jithesh Kottur ◽  
Naveen Narayanan ◽  
Deepak T. Nair
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Serine Residue ◽  
Mutator Activity ◽  
Dna Polymerase Iv

Bactericidal action of peptide antibiotic AS-48 against Escherichia coli K-12

1989 ◽  
Vol 35 (2) ◽  
pp. 318-321 ◽  
Author(s):  
A. Gálvez ◽  
E. Valdivia ◽  
M. Martínez ◽  
M. Maqueda
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Cell Growth ◽  
Mode Of Action ◽  
Cytoplasmic Membrane ◽  
Peptide Antibiotic ◽  
Biosynthetic Pathways ◽  
Bactericidal Action ◽  
Cell Wall Synthesis ◽  
K 12

Peptide antibiotic AS-48 exerts a bactericidal mode of action on exponential cultures of Escherichia coli K-12 through a multi-hit kinetics interaction. AS-48 causes a parallel and gradual cessation of all biosynthetic pathways monitored (protein, RNA, DNA, and cell wall synthesis), the rate of incorporation of labeled precursors, the rate of O2 consumption, and cell growth. These effects have been attributed to alterations of cytoplasmic membrane functions.Key words: Escherichia coli, peptide antibiotic, bactericide.

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