Investigation of Linezolid Resistance in Staphylococci and Enterococci

The objective of this study was to investigate an apparent increase in linezolid-nonsusceptible staphylococci and enterococci following a laboratory change in antimicrobial susceptibility testing from disk diffusion to an automated susceptibility testing system. Isolates with nonsusceptible results (n= 27) from Vitek2 were subjected to a battery of confirmatory testing which included disk diffusion, Microscan broth microdilution, Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution, gradient diffusion (Etest), 23S rRNA gene sequencing, andcfrPCR. Our results show that there is poor correlation between methods and that only 70 to 75% of isolates were confirmed as linezolid resistant with alternative phenotypic testing methods (disk diffusion, Microscan broth microdilution, CLSI broth microdilution, and Etest). 23S rRNA gene sequencing identified mutations previously associated with linezolid resistance in 16 (59.3%) isolates, and thecfrgene was detected in 3 (11.1%) isolates. Mutations located at positions 2576 and 2534 of the 23S rRNA gene were most common. In addition, two previously undescribed variants (at positions 2083 and 2345 of the 23S rRNA gene) were also identified and may contribute to linezolid resistance.

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Infective Arthritis: Bacterial 23S rRNA Gene Sequencing as a Supplementary Diagnostic Method

The Open Microbiology Journal ◽

10.2174/1874285800802010085 ◽

2008 ◽

Vol 2 (1) ◽

pp. 85-88 ◽

Cited By ~ 5

Author(s):

Claus Moser ◽

Keld Andresen ◽

Anne Kjerulf ◽

Suheil Salamon ◽

Michael Kemp ◽

...

Keyword(s):

Diagnostic Method ◽

Gene Sequencing ◽

23S Rrna ◽

Rrna Gene ◽

23S Rrna Gene ◽

Rrna Gene Sequencing ◽

Infective Arthritis

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Corrigendum to “Linezolid resistance in Staphylococcus epidermidis associated with a G2603T mutation in the 23S rRNA gene” [Int. J. Antimicrob. Agents 34 (2009) 281–282]

International Journal of Antimicrobial Agents ◽

10.1016/j.ijantimicag.2011.11.001 ◽

2012 ◽

Vol 39 (2) ◽

pp. 186

Author(s):

Nilton Lincopan ◽

Lara M. de Almeida ◽

Maria R. Elmor de Araújo ◽

Elsa M. Mamizuka

Keyword(s):

Staphylococcus Epidermidis ◽

23S Rrna ◽

Rrna Gene ◽

Linezolid Resistance ◽

23S Rrna Gene

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Characterization of Lactic Bacteria Isolated from Raw Milk and Their Antibacterial Activity against Bacteria as the Cause of Clinical Bovine Mastitis

Journal of Food Quality ◽

10.1155/2021/6466645 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Nada K. Alharbi ◽

Albandary Nasser Alsaloom

Keyword(s):

Bovine Mastitis ◽

16S Rrna Gene Sequencing ◽

Raw Milk ◽

Antibacterial Activities ◽

Antagonistic Activity ◽

23S Rrna ◽

Rrna Gene ◽

Technological Properties ◽

23S Rrna Gene ◽

Rrna Gene Sequencing

The objectives of this study were the selection of lactic acid bacteria (LAB) isolated from raw milk and studying their technological properties and antibacterial activities against bacteria as the cause of cattle mastitis. Biochemical and molecular identification using 16S–23S rRNA gene spacer analysis and 16S rRNA gene sequencing highlighted the presence of three species: Lactiplantibacillus plantarum, Lactococcus lactis, and Levilactobacillus brevis. The enzymatic characterization followed by the determination of technofunctional properties showed that LAB strains did not exhibit any hemolytic effect and were able to produce protease and lipase enzymes. Isolates showed very high antagonistic activity against Gram-positive and Gram-negative bacteria by producing H2O2, bacteriocin(s), and organic acid(s). APIZYM micromethod demonstrated that all selected strains are capable of producing valine arylamidase, cystine arylamidase, N-acetyl-β-glucosaminidase, and ᾳ-mannosidase. The antibiotic susceptibility assay showed that all selected strains were sensible to the majority of tested antibiotics. Based on these results, it can be concluded that the technological properties of the selected LAB allow considering their industrial use in order to formulate bioactive functional foods or drug(s).

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Linezolid Resistance in SequentialStaphylococcus aureusIsolates Associated with a T2500A Mutation in the 23S rRNA Gene and Loss of a Single Copy of rRNA

The Journal of Infectious Diseases ◽

10.1086/421471 ◽

2004 ◽

Vol 190 (2) ◽

pp. 311-317 ◽

Cited By ~ 159

Author(s):

Venkata G. Meka ◽

Satish K. Pillai ◽

George Sakoulas ◽

Christine Wennersten ◽

Lata Venkataraman ◽

...

Keyword(s):

Single Copy ◽

23S Rrna ◽

Rrna Gene ◽

Linezolid Resistance ◽

23S Rrna Gene

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Rapid Detection and Estimation by Pyrosequencing of 23S rRNA Genes with a Single Nucleotide Polymorphism Conferring Linezolid Resistance in Enterococci

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.47.11.3620-3622.2003 ◽

2003 ◽

Vol 47 (11) ◽

pp. 3620-3622 ◽

Cited By ~ 68

Author(s):

Alistair Sinclair ◽

Catherine Arnold ◽

Neil Woodford

Keyword(s):

Fragment Length Polymorphism ◽

Rrna Genes ◽

23S Rrna ◽

Rrna Gene ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Linezolid Resistance ◽

Gene Copies ◽

23S Rrna Gene ◽

23S Rrna Genes

ABSTRACT Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygous for either G2576 or T2576 or heterozygous for this mutation. A good correlation was found between linezolid MICs and the number of 23S rRNA gene copies carrying the mutation.

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Analysis of methods commonly used for glycopeptide and oxazolidinone susceptibility testing in Enterococcus faecium isolates

Journal of Medical Microbiology ◽

10.1099/jmm.0.016444-0 ◽

2010 ◽

Vol 59 (6) ◽

pp. 672-678 ◽

Cited By ~ 7

Author(s):

Giammarco Raponi ◽

Maria Cristina Ghezzi ◽

Giovanni Gherardi ◽

Giulia Lorino ◽

Giordano Dicuonzo

Keyword(s):

Enterococcus Faecium ◽

Susceptibility Testing ◽

23S Rrna ◽

Rrna Gene ◽

Disc Diffusion ◽

Pcr Rflp ◽

23S Rrna Gene ◽

Vitek 2 ◽

Resistance Rates ◽

The Phoenix

The susceptibility to teicoplanin, vancomycin and linezolid of 30 clinical isolates of Enterococcus faecium was tested by Vitek 2, Phoenix, Etest, broth microdilution and disc diffusion tests. The vanA and vanB resistance genes and the 23S rRNA gene G2576T mutation were detected by PCR and PCR-RFLP, respectively. Resistance rates to teicoplanin ranged from 3 % for Vitek 2 to 57.6 % for the Phoenix test, and those to vancomycin ranged from 56.7 % for Vitek 2 to 86.7 % for the Phoenix test. Only two out of 25 strains carrying the vanA gene were univocally recognized as the VanA phenotype. The only strain with the G2576T mutation did not carry the vanA gene and showed resistance to linezolid by the disc diffusion, Vitek 2 and broth dilution methods (MIC >8 μg ml−1), but was susceptible when tested with the Phoenix test and Etest (MIC ≤4 μg ml−1). Therefore, the resistance to glycopeptides and linezolid was not univocally detected by the susceptibility testing methods used in this study.

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Linezolid Resistance in Staphylococcus aureus: Gene Dosage Effect, Stability, Fitness Costs, and Cross-Resistances

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01098-07 ◽

2008 ◽

Vol 52 (4) ◽

pp. 1570-1572 ◽

Cited By ~ 88

Author(s):

Silke Besier ◽

Albrecht Ludwig ◽

Johannes Zander ◽

Volker Brade ◽

Thomas A. Wichelhaus

Keyword(s):

Staphylococcus Aureus ◽

Gene Dosage ◽

Single Point ◽

23S Rrna ◽

Cross Resistance ◽

Single Point Mutation ◽

Rrna Gene ◽

Gene Dosage Effect ◽

Linezolid Resistance ◽

23S Rrna Gene

ABSTRACT Linezolid resistance in Staphylococcus aureus is typically associated with mutations in the 23S rRNA gene. Here we show that the accumulation of a single point mutation, G2576T, in the different copies of this gene causes stepwise increases in resistance, impairment of the biological fitness, and cross-resistance to quinupristin-dalfopristin and chloramphenicol.

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Deletion of One 23S rRNA Gene (rrl) Copy Contributes to the Development of Linezolid Resistance in Staphylococcus warneri

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01139-18 ◽

2018 ◽

Vol 62 (11) ◽

Author(s):

Caroline Rouard ◽

Florence Doucet-Populaire ◽

Christelle Guillet-Caruba ◽

Millie Villet ◽

Nadège Bourgeois-Nicolaos

Keyword(s):

23S Rrna ◽

Rrna Gene ◽

Staphylococcus Warneri ◽

Linezolid Resistance ◽

23S Rrna Gene

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Impact of Clarithromycin Resistance on Eradication of Helicobacter pylori in Infected Adults

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.49.4.1600-1603.2005 ◽

2005 ◽

Vol 49 (4) ◽

pp. 1600-1603 ◽

Cited By ~ 46

Author(s):

Jong Hwa Lee ◽

Ji-Hyun Shin ◽

Im Hwan Roe ◽

Seung Ghyu Sohn ◽

Jung Hun Lee ◽

...

Keyword(s):

Helicobacter Pylori ◽

Drug Therapy ◽

Antimicrobial Susceptibility ◽

Susceptibility Testing ◽

Gastric Biopsy ◽

Antimicrobial Susceptibility Testing ◽

23S Rrna ◽

Rrna Gene ◽

Clarithromycin Resistance ◽

23S Rrna Gene

ABSTRACT The outcome of Helicobacter pylori infection was analyzed in 114 dyspeptic patients treated with triple-drug therapy including clarithromycin. Clarithromycin resistance (in 20.2% of our isolates) was mainly caused by an A2142G mutation in the 23S rRNA gene of H. pylori. H. pylori eradication was obtained in all patients with clarithromycin-susceptible isolates but not in any patients with clarithromycin-resistant isolates (P = 0.0001). Therefore, it would be useful to conduct H. pylori antimicrobial susceptibility testing of the first gastric biopsy culture before choosing the first three drugs for therapy of infected patients.

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