Differentiation of Campylobacter species by protein banding patterns in polyacrylamide slab gels.

The electrophoretic mobilities of non-specific esterases in vertical polyacrylamide slab gels were determined for 184 strains of staphylococci, representing a total of 18 proposed species and subspecies. Markedly uniform esterase patterns were seen within species demonstrating a high degree of human host specificity, while those species demonstrating a wide host range were polytypic and often showed considerable polymorphism. The unique banding patterns found in several species indicate that this technique may serve as a valuable aid to existing taxonomic schemes. Starch gel electrophoresis of representative strains usually produced sharper esterase bands than were found with polyacrylamide electrophoresis. However, the additional molecular-sieving effect produced by the polyacrylamide gels differentiated esterases to a greater extent.

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Advances in imaging SIMS studies of stained and BrdU-labelled human metaphase chromosomes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141032 ◽

1995 ◽

Vol 53 ◽

pp. 932-933

Author(s):

R. Levi-Setti ◽

J. M. Chabala ◽

R. Espinosa ◽

M. M. Le Beau

Keyword(s):

High Performance ◽

Secondary Ion Mass Spectrometry ◽

Analytical Sensitivity ◽

Metaphase Chromosomes ◽

Banding Patterns ◽

Sector Mass Spectrometer ◽

Staining Methods ◽

The University ◽

Secondary Ion ◽

Better Than

We have shown previously that isotope-labelled nucleotides in human metaphase chromosomes can be detected and mapped by imaging secondary ion mass spectrometry (SIMS), using the University of Chicago high resolution scanning ion microprobe (UC SIM). These early studies, conducted with BrdU- and 14C-thymidine-labelled chromosomes via detection of the Br and 28CN- (14C14N-> labelcarrying signals, provided some evidence for the condensation of the label into banding patterns along the chromatids (SIMS bands) reminiscent of the well known Q- and G-bands obtained by conventional staining methods for optical microscopy. The potential of this technique has been greatly enhanced by the recent upgrade of the UC SIM, now coupled to a high performance magnetic sector mass spectrometer in lieu of the previous RF quadrupole mass filter. The high transmission of the new spectrometer improves the SIMS analytical sensitivity of the microprobe better than a hundredfold, overcoming most of the previous imaging limitations resulting from low count statistics.

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The staining pattern of the tropomyosin sequence is displayed in a new paracrystal

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142372 ◽

1986 ◽

Vol 44 ◽

pp. 150-151

Author(s):

M.K. Lamvik ◽

L.L. Klatt

Keyword(s):

Amino Acid ◽

Amino Acid Sequence ◽

Staining Pattern ◽

Banding Patterns ◽

Mass Thickness ◽

New Form

Tropomyosin paracrystals have been used extensively as test specimens and magnification standards due to their clear periodic banding patterns. The paracrystal type discovered by Ohtsuki1 has been of particular interest as a test of unstained specimens because of alternating bands that differ by 50% in mass thickness. While producing specimens of this type, we came across a new paracrystal form. Since this new form displays aligned tropomyosin molecules without the overlaps that are characteristic of the Ohtsuki-type paracrystal, it presents a staining pattern that corresponds to the amino acid sequence of the molecule.

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Campylobacter species distribution in outdoor pigs

10.31274/safepork-180809-479 ◽

2003 ◽

Author(s):

A. N. Jensen ◽

E. M. Nielsen

Keyword(s):

Species Distribution ◽

Campylobacter Species

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Diversity of Common Bean Landraces Collected in the Western and Eastern Carpatien

Czech Journal of Genetics and Plant Breeding ◽

10.17221/3723-cjgpb ◽

2011 ◽

Vol 39 (No. 3) ◽

pp. 73-83 ◽

Cited By ~ 3

Author(s):

O. Horňáková ◽

M. Závodná ◽

M. Žáková ◽

J. Kraic ◽

F. Debre

Keyword(s):

Cluster Analysis ◽

Common Bean ◽

Morphological Characters ◽

Molecular Data ◽

Seed Traits ◽

Phaseolus Vulgaris L ◽

Growth Type ◽

Banding Patterns ◽

Polymorphism Detection ◽

Thousand Seed Weight

The study of diversity in common bean was based on morphological and agronomical characteristics, differentiation of collected accessions by morphological and molecular markers, detection of genetic variation, and duplicates detection in bean landraces. The analysed 82 accessions of common bean (Phaseolus vulgaris L.) were collected in the Western andEastern Carpatien as landrace mixtures. Their seeds were segregated and pooled according to their characteristics; they were further multiplicated, and introduced into the collection. An extensive variation in plant and seed traits was discovered in thirty-three morphological and agronomical characteristics. Nevertheless, some of the accessions were identical in these characteristics. Cluster analysis grouped genotypes into two main branches, reflecting the growth type, seed size parameters, and thousand-seed weight. Molecular differentiation studies were performed by multilocus polymorphism detection in microsatellite and minisatellite DNA regions. Cluster analysis based on molecular data also grouped genotypes but no linkage to morphological traits was revealed. Bean accessions with very similar or identical morphological characters were clearly distinguished by DNA banding patterns. The presence of duplicates was excluded.  

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