Specific detection and typing of adenovirus types 40 and 41 in stool specimens by dot-blot hybridization.

1985 ◽  
Vol 22 (6) ◽  
pp. 934-939 ◽  
Author(s):  
A H Kidd ◽  
E H Harley ◽  
M J Erasmus
Keyword(s):  
Blot Hybridization ◽  
Dot Blot ◽  
Specific Detection ◽  
Dot Blot Hybridization ◽  
Stool Specimens

scholarly journals Comparative Infection Progress Analysis of Lettuce big-vein virus and Mirafiori lettuce virus in Lettuce Crops by Developed Molecular Diagnosis Techniques

2004 ◽  
Vol 94 (5) ◽  
pp. 470-477 ◽  
Author(s):  
Jose A. Navarro ◽  
Francisco Botella ◽  
Antonio Maruhenda ◽  
Pedro Sastre ◽  
M. Amelia Sánchez-Pina ◽  
...  
Keyword(s):  
Blot Hybridization ◽  
Hybridization Technique ◽  
Dot Blot ◽  
Specific Detection ◽  
Chain Reaction ◽  
Dot Blot Hybridization ◽  
Production Area ◽  
Olpidium Brassicae ◽  
Polymerase Chain ◽  
Production Areas

Nonisotopic molecular dot blot hybridization technique and multiplex reverse transcription-polymerase chain reaction assay for the specific detection of Lettuce big-vein virus (LBVV) and Mirafiori lettuce virus (MiLV) in lettuce tissue were developed. Both procedures were suitable for the specific detection of both viruses in a range of naturally infected lettuce plants from various Spanish production areas and seven different cultivars. The study of the distribution of both viruses in the plant revealed that the highest concentration of LBVV and MiLV occurred in roots and old leaves, respectively. LBVV infection progress in a lettuce production area was faster than that observed for MiLV. In spite of different rates of virus infection progress, most lettuce plants became infected with both viruses about 100 days posttransplant. The appearance of both viruses in lettuce crops was preceded by a peak in the concentration of resting spores and zoosporangia of the fungus vector Olpidium brassicae in lettuce roots.

scholarly journals Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe

1992 ◽  
Vol 108 (1) ◽  
pp. 175-184 ◽  
Author(s):  
J. Fernández ◽  
A. Sandino ◽  
A. Yudelevich ◽  
L. F. Avendaño ◽  
A. Venegas ◽  
...  
Keyword(s):  
Blot Hybridization ◽  
Hybridization Assay ◽  
Hybridization Method ◽  
Dot Blot ◽  
Laboratory Procedure ◽  
Dot Blot Hybridization ◽  
Gene Coding ◽  
Dot Hybridization ◽  
Stool Specimens ◽  
Higher Sensitivity

SUMMARYA synthetic oligodeoxynucletide of 40 nucleotides corresponding to nucleotides 33–72 of the gene coding for the viral protein VP7 of rotavirus, was used as a nucleic acid probe to develop a non-radiactive hybridization method for rotavirus detection. The probe was labelled at the 3' end with biotin-7-dATP. The sensitivity and specificity of the dot blot hybridization assay for rotavirus detection was evaluated with 303 stool specimens. The results indicate that the hybridization assay has a higher sensitivity than both PAGE and EIA. Among the rotavirus strains tested 37 different electropherotypes were found. The results suggest that rotavirus diagnosis by dot hybridization using a non-radioactive probe may become routine laboratory procedure because it is simple, highly specific and very sensitive.

Feasibility of Qualitative Testing of BCR-ABL and JAK2 V617F in Suspected Myeloproliperative Neoplasm (MPN) Using RT-PCR Reversed Dot Blot Hybridization (RT-PCR RDB)

2019 ◽  
Vol 19 (4) ◽  
pp. 220-227
Author(s):  
Najmiatul Masykura ◽  
Ummu Habibah ◽  
Siti Fatimah Selasih ◽  
Soegiarto Gani ◽  
Cosphiadi Irawan ◽  
...  
Keyword(s):  
Blot Hybridization ◽  
Jak2 V617f ◽  
Rt Pcr ◽  
Dot Blot ◽  
Dot Blot Hybridization

Prevalence of HPV in a Melbourne Female STD Population: Comparison of RNA and DNA Probes in Detecting HPV by Dot Blot Hybridization

1993 ◽  
Vol 4 (3) ◽  
pp. 159-164 ◽  
Author(s):  
A J Borg ◽  
G Medley ◽  
S M Garland
Keyword(s):  
Past History ◽  
Blot Hybridization ◽  
Condylomata Acuminata ◽  
Dot Blot ◽  
Hpv Dna ◽  
Sexually Transmitted ◽  
Dot Blot Hybridization ◽  
History Of ◽  
Cytological Features ◽  
Dna Types

A total of 377 women, consecutively selected as first attenders to a sexually transmitted diseases clinic in Melbourne, Australia, were examined for overt Condylomata acuminata and were screened for genital HPV DNA types 6, 11, 16, 18, 31, 33 and (35) using 2 dot blot hybridization methods. Overall, there was a 90% positivity correlation between the 2 methods with HPV DNA being detected in 12% of ectocervical samples. Overt warts were found in 15% of the women and HPV DNA was detected at the cervix in 35% with cytology predicting HPV with or without dysplasia in 27%. Thirteen percent had a past history of warts but none on examination and HPV DNA was evident in 16% while 18% had cytological features of HPV. Those with no warts evident and no past history of warts had both HPV DNA and cytological features of HPV in 7%.

Detection of herpes simplex virus and adenovirus DNA by dot blot hybridization using in vitro synthesized RNA transcripts

1986 ◽  
Vol 13 (4) ◽  
pp. 291-299 ◽  
Author(s):  
Volker Schuster ◽  
Bertfried Matz ◽  
Helga Wiegand ◽  
Brigitte Traub ◽  
Dieter Neumann-Haefelin
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Blot Hybridization ◽  
Dot Blot ◽  
Rna Transcripts ◽  
Dot Blot Hybridization ◽  
Simplex Virus
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