Large production of cholera toxin by Vibrio cholerae O1 in yeast extract peptone water.

1987 ◽  
Vol 25 (12) ◽  
pp. 2314-2316 ◽  
Author(s):  
M Iwanaga ◽  
T Kuyyakanond
Keyword(s):  
Cholera Toxin ◽  
Vibrio Cholerae ◽  
Yeast Extract ◽  
Peptone Water ◽  
Vibrio Cholerae O1 ◽  
Large Production

Comparison of Methods for Recovering Vibrio cholerae O1 from Ice

2006 ◽  
Vol 69 (1) ◽  
pp. 217-220 ◽  
Author(s):  
RITA CAVA ◽  
ELBA SANGRONIS ◽  
FULGENCIO MARIN-INIESTA
Keyword(s):  
Vibrio Cholerae ◽  
Yeast Extract ◽  
Mineral Water ◽  
Most Probable Number ◽  
Comparison Of Methods ◽  
Probable Number ◽  
Alkaline Peptone Water ◽  
Nacl Concentration ◽  
Peptone Water ◽  
Vibrio Cholerae O1

Alkaline peptone water (1% peptone, 1% NaCl, pH 8.5) and Trypticase soy yeast extract broth (TSYB) supplemented with 2.5% NaCl (pH 8.5) or 1% NaCl (pH 7.5) were evaluated as enrichment broths for the isolation of Vibrio cholerae O1 from ice. Thirty samples of sterile and nonsterile mineral water were inoculated with cell suspensions of this bacterium, quickly frozen, and stored for 3 days at −18°C. After thawing, samples were analyzed by a three-tube most-probable-number technique. Incubation in TSYB with 2.5% NaCl (pH 8.5) for 18 h at 37°C yielded the highest recovery of V. cholerae O1 cells (P < 0.05), a result that might be attributable to the nutrients and to the NaCl concentration of the TSYB, both of which would promote V. cholerae O1 growth and prevent the growth of competitive microbiota.

scholarly journals Variation in Epitopes of the B Subunit of El Tor and Classical Biotype Vibrio cholerae O1 Cholera Toxin

Microbiology ◽  
1989 ◽  
Vol 135 (5) ◽  
pp. 1195-1200 ◽  
Author(s):  
M. L. Tamplin ◽  
M. K. Ahmed ◽  
R. Jalali ◽  
R. R. Colwell
Keyword(s):  
Cholera Toxin ◽  
Vibrio Cholerae ◽  
B Subunit ◽  
Vibrio Cholerae O1 ◽  
El Tor

scholarly journals Influence of the copepod Mesocyclops longisetus (Crustacea: Cyclopidae) on the survival of Vibrio cholerae O1 in fresh water

1996 ◽  
Vol 12 (4) ◽  
pp. 551-554 ◽  
Author(s):  
Daniela Bastos Araújo ◽  
Suzana Cláudia Silveira Martins ◽  
Laurênia Maria Braga de Albuquerque ◽  
Ernesto Hofer
Keyword(s):  
Aedes Aegypti ◽  
Vibrio Cholerae ◽  
Fresh Water ◽  
Room Temperature ◽  
The State ◽  
Bacterial Suspension ◽  
Alkaline Peptone Water ◽  
Colony Forming Units ◽  
Peptone Water ◽  
Vibrio Cholerae O1

In an experimental microcosm, an analysis was performed of the influence exerted by freshwater Mesocyclops longisetus copepods on the survival of Vibrio cholerae O1 serovar Inaba. In the State of Ceará, copepods are used in the control of Aedes aegypti larvae. The system consisted of water with a salinity of 0.27‰ and pH 7.5, which after sterilizing filtration was distributed into seven flasks with a volume of 400 ml; in each of six flasks, 10 live copepods were inoculated along with 1 ml of an 8-hour culture of Vibrio cholerae O1 at 37ºC in Alkaline Peptone Water, resulting in a concentration of 3.80x10(4) colony-forming units. The control flask contained only the water with the same bacterial suspension. The system was maintained for six days at room temperature (25-28ºC), and daily duplicate counts were performed in TCBS Agar. Results confirmed a clear association between Vibrio cholerae O1 and the live copepods, based on survival of the bacteria at compatible levels with the initial inoculation until the sixth day of the analysis.

scholarly journals Cholera Toxin Production by the El Tor Variant of Vibrio cholerae O1 Compared to Prototype El Tor and Classical Biotypes

2010 ◽  
Vol 48 (11) ◽  
pp. 4283-4286 ◽  
Author(s):  
J. Ghosh-Banerjee ◽  
M. Senoh ◽  
T. Takahashi ◽  
T. Hamabata ◽  
S. Barman ◽  
...  
Keyword(s):  
Cholera Toxin ◽  
Vibrio Cholerae ◽  
Toxin Production ◽  
Vibrio Cholerae O1 ◽  
El Tor

Classical RS1 and environmental RS1 elements in Vibrio cholerae O1 El Tor strains harbouring a tandem repeat of CTX prophage: revisiting Mozambique in 2005

2010 ◽  
Vol 59 (3) ◽  
pp. 302-308 ◽  
Author(s):  
Seon Young Choi ◽  
Je Hee Lee ◽  
Eun Jin Kim ◽  
Hye Ri Lee ◽  
Yoon-Seong Jeon ◽  
...  
Keyword(s):  
Cholera Toxin ◽  
Vibrio Cholerae ◽  
Tandem Repeat ◽  
Small Chromosome ◽  
Classical Type ◽  
Large Chromosome ◽  
New Type ◽  
Vibrio Cholerae O1 ◽  
Ctx Prophage ◽  
El Tor

Currently, Vibrio cholerae O1 serogroup biotype El Tor strains producing classical type cholera toxin (altered strains or El Tor variants) are prevalent in Asia and in Mozambique. Mozambican strains collected in 2004 contained a tandem repeat of CTX prophage on the small chromosome and each CTX prophage harboured the classical rstR and classical ctxB. We found that the majority of the strains collected in 2005 in Mozambique contained extra elements on the large chromosome in addition to the tandem repeat of CTX prophage on the small chromosome. New type RS1 elements RS1cla and RS1env, and a CTXenv with rstR env and the classical ctxB were identified on the large chromosome of the Mozambican isolates collected in 2005.

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