Influence of the copepod Mesocyclops longisetus (Crustacea: Cyclopidae) on the survival of Vibrio cholerae O1 in fresh water

In an experimental microcosm, an analysis was performed of the influence exerted by freshwater Mesocyclops longisetus copepods on the survival of Vibrio cholerae O1 serovar Inaba. In the State of Ceará, copepods are used in the control of Aedes aegypti larvae. The system consisted of water with a salinity of 0.27‰ and pH 7.5, which after sterilizing filtration was distributed into seven flasks with a volume of 400 ml; in each of six flasks, 10 live copepods were inoculated along with 1 ml of an 8-hour culture of Vibrio cholerae O1 at 37ºC in Alkaline Peptone Water, resulting in a concentration of 3.80x10(4) colony-forming units. The control flask contained only the water with the same bacterial suspension. The system was maintained for six days at room temperature (25-28ºC), and daily duplicate counts were performed in TCBS Agar. Results confirmed a clear association between Vibrio cholerae O1 and the live copepods, based on survival of the bacteria at compatible levels with the initial inoculation until the sixth day of the analysis.

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Comparison of Methods for Recovering Vibrio cholerae O1 from Ice

Journal of Food Protection ◽

10.4315/0362-028x-69.1.217 ◽

2006 ◽

Vol 69 (1) ◽

pp. 217-220 ◽

Cited By ~ 2

Author(s):

RITA CAVA ◽

ELBA SANGRONIS ◽

FULGENCIO MARIN-INIESTA

Keyword(s):

Vibrio Cholerae ◽

Yeast Extract ◽

Mineral Water ◽

Most Probable Number ◽

Comparison Of Methods ◽

Probable Number ◽

Alkaline Peptone Water ◽

Nacl Concentration ◽

Peptone Water ◽

Vibrio Cholerae O1

Alkaline peptone water (1% peptone, 1% NaCl, pH 8.5) and Trypticase soy yeast extract broth (TSYB) supplemented with 2.5% NaCl (pH 8.5) or 1% NaCl (pH 7.5) were evaluated as enrichment broths for the isolation of Vibrio cholerae O1 from ice. Thirty samples of sterile and nonsterile mineral water were inoculated with cell suspensions of this bacterium, quickly frozen, and stored for 3 days at −18°C. After thawing, samples were analyzed by a three-tube most-probable-number technique. Incubation in TSYB with 2.5% NaCl (pH 8.5) for 18 h at 37°C yielded the highest recovery of V. cholerae O1 cells (P < 0.05), a result that might be attributable to the nutrients and to the NaCl concentration of the TSYB, both of which would promote V. cholerae O1 growth and prevent the growth of competitive microbiota.

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Large production of cholera toxin by Vibrio cholerae O1 in yeast extract peptone water.

Journal of Clinical Microbiology ◽

10.1128/jcm.25.12.2314-2316.1987 ◽

1987 ◽

Vol 25 (12) ◽

pp. 2314-2316 ◽

Cited By ~ 29

Author(s):

M Iwanaga ◽

T Kuyyakanond

Keyword(s):

Cholera Toxin ◽

Vibrio Cholerae ◽

Yeast Extract ◽

Peptone Water ◽

Vibrio Cholerae O1 ◽

Large Production

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Demonstration of viable but nonculturable Vibrio cholerae O1 in fresh water environment of India using ciprofloxacin DFA-DVC method

Letters in Applied Microbiology ◽

10.1111/j.1472-765x.2011.03077.x ◽

2011 ◽

Vol 53 (1) ◽

pp. 124-126 ◽

Cited By ~ 11

Author(s):

A. Mishra ◽

N. Taneja ◽

M. Sharma

Keyword(s):

Vibrio Cholerae ◽

Fresh Water ◽

Water Environment ◽

Viable But Nonculturable ◽

Vibrio Cholerae O1 ◽

Fresh Water Environment

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Laboratory Study of Vibrio cholerae O1 Survival on Three Types of Boiled Rice (Oryza sativa L.) Held at Room Temperature

Journal of Food Protection ◽

10.4315/0362-028x-71.12.2453 ◽

2008 ◽

Vol 71 (12) ◽

pp. 2453-2459 ◽

Cited By ~ 4

Author(s):

JOHN TANG YEW HUAT ◽

YAP KOK LEONG ◽

HING HIANG LIAN

Keyword(s):

Vibrio Cholerae ◽

Room Temperature ◽

Brown Rice ◽

Rice Grain ◽

White Rice ◽

Rice Grains ◽

Cooked Rice ◽

Glutinous Rice ◽

Vibrio Cholerae O1 ◽

Grain Surface

This study examined whether the survival of Vibrio cholerae O1 on contaminated cooked rice was influenced by the type of rice. Vibrios survived unchanged on clumps of glutinous white rice (wet, grains adhered) held at room temperature for 24 h. On nonglutinous white rice (slightly moist, grains separate), 30% viable vibrios remained at 24 h. On nonglutinous brown rice (moist, separate, covered with a mucus-like substance), the number of vibrios increased 2.7-fold at 24 h. Survival rates of vibrios on the surfaces of a row of five cooked rice grains after 2 h of exposure at room temperature were 86, 29, 12, and 4% for glutinous rice, white rice, and the endosperm and pericarp of brown rice, respectively. (Each boiled brown rice grain surface was partly pericarp and partly endosperm, which became exposed by a rupture of the pericarp.) Covering each inoculated grain with a similar cooked rice grain surface increased the corresponding figures to 93, 99, 60, and 94%. Scanning electron microscopy revealed that each type of cooked grain surface possessed a distinct microtopography. For example, the surfaces of glutinous rice grains consisted of separated overlapping strips with many holes, while the pericarps of brown rice were flat interspersed with small pits. In conclusion, each type of boiled rice produced a distinct survival pattern of V. cholerae O1 caused by both the distinct gross features and the fine surface characteristics of the rice. The significance of this finding is that the type of rice consumed can be a factor in cholera transmission by contaminated rice.

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Two Different Diagnosis Methods For The detection of cholera toxin production From Vibrio cholerae isolated from different areas in Iraq

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v34i2.636 ◽

2010 ◽

Vol 34 (2) ◽

pp. 95-98

Author(s):

Atheer Abdulrazzaq

Keyword(s):

Cholera Toxin ◽

Vibrio Cholerae ◽

Toxin Production ◽

Secretory Diarrhea ◽

Chain Reaction ◽

Central Public Health ◽

Alkaline Peptone Water ◽

Peptone Water ◽

Polymerase Chain ◽

Positive Results

Fifty isolates of Vibrio cholerae obtained from different areas in Iraq from patients with acute secretory diarrhea were diagnosed serologically in central public health laboratory(CPHL), Two different methods were used for detection cholera toxin production ,the first based on the degradation of Nicotin Amide Adinine Dinucleotide( NAD) by Cholera toxin (CT). and the second was by detection CT gene by polymerase chain reaction (PCR).All Vibrio cholerae were cultured in alkaline peptone water, out of 50 isolates 46 (92%) were positive when we used NAD for the detection of cholera toxin(CT).Whereas all isolates gave positive results by PCR. From these data we conclude that the degradation of NAD by CT is simple and can be carried out in small laboratories, it is also easy to perform, and gives reproducible results, while PCR provides a more sensitive and specific assay for rapid diagnosis of cholera than currently available methods.

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In vivo Production of Soluble Inorganic Pyrophosphatases in Two Strains of Vibrio cholerae

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v24i1.1235 ◽

1970 ◽

Vol 24 (1) ◽

pp. 38-41

Author(s):

Taslima Taher Lina ◽

Mohammad Ilias

Keyword(s):

Vibrio Cholerae ◽

Specific Activity ◽

Experimental Conditions ◽

Environmental Strain ◽

Cell Extracts ◽

Atcc Strain ◽

The Family ◽

Effects Of Ph ◽

Vibrio Cholerae O1

The in vivo production of soluble inorganic pyrophosphatases (PPases) was investigated in two strains, namely, Vibrio cholerae EM 004 (environmental strain) and Vibrio cholerae O1 757 (ATCC strain). V. cholerae is known to contain both family I and family II PPase coding sequences. The production of family I and family II PPases were determined by measuring the enzyme activity in cell extracts. The effects of pH, temperature, salinity of the growth medium on the production of soluble PPases were studied. In case of family I PPase, V. cholerae EM 004 gave the highest specific activity at pH 9.0, with 2% NaCl + 0.011% NaF and at 37°C. The strain V. cholerae O1 757 gave the highest specific activity at pH 9.0, with media containing 0% NaCl and at 37°C. On the other hand, under all the conditions family II PPase did not give any significant specific activity, suggesting that the family II PPase was not produced in vivo in either strains of V. cholerae under different experimental conditions. Keywords: Vibrio cholerae, Pyrophosphatases (PPases), Specific activityDOI: http://dx.doi.org/10.3329/bjm.v24i1.1235 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 38-41

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