scholarly journals Possibility of diagnosing meningitis by gas chromatography: cryptococcal meningitis

1976 ◽  
Vol 3 (3) ◽  
pp. 239-245
Author(s):  
D Schlossberg ◽  
J B Brooks ◽  
J Shulman
Keyword(s):  
Gas Chromatography ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Cryptococcus Neoformans ◽  
Electron Capture ◽  
Cryptococcal Meningitis ◽  
Viral Infections ◽  
Viral Meningitis ◽  
Gas Liquid Chromatography ◽  
Cryptococcal Infection

Cerebrospinal fluid (CSF) from eight patients with cryptococcal meningitis, from ten patients with viral meningitis, and from four control patients without meningitis were analyzed by electron-capture gas-liquid chromatography (EC-GLC). All cryptococcal specimens had similar EC-GLC profiles, and these differed from those of the controls. Viral EC-GLC patterns were different from those obtained with specimens from the patients with cryptococcal infection and from the controls. In addition, specimens from patients with various types of viral infections gave profiles that differed from each other. Two normal CSFs were inoculated with Cryptococcus neoformans; aliquots of these cultures showed an EC-GLC pattern very similar to that seen in CSF of patients with cryptococcal meningitis. The EC-GLC procedure is rapid, reproducible, and easy to perform and may hold promise as an additional aid in the diagnosis of cryptococcal infection.

scholarly journals Rapid diagnosis of lymphocytic meningitis by frequency-pulsed electron capture gas-liquid chromatography: differentiation of tuberculous, cryptococcal, and viral meningitis

1977 ◽  
Vol 6 (1) ◽  
pp. 27-32
Author(s):  
R B Craven ◽  
J B Brooks ◽  
D C Edman ◽  
J D Converse ◽  
J Greenlee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Electron Capture ◽  
Viral Meningitis ◽  
Rapid Diagnosis ◽  
Gas Liquid Chromatography ◽  
Therapeutic Approaches ◽  
New Therapeutic Approaches ◽  
Lymphocytic Meningitis

Cerebrospinal fluid specimens from patients with tuberculous (17 cases), cryptococcal (15 cases), and viral (14 cases) meningitis were analyzed by frequency-pulsed electron capture gas-liquid chromatography and mass spectrometry. Compounds that disappeared after therapy were found to be present in each of these specimens and were not detected in controls. They occurred in repetitive patterns such that these three types of meningitis could be rapidly distinguished. The compound associated with tuberculous meningitis has been tentatively identified. These finding have implications for rapid diagnosis, pathophysiological studies, and possible new therapeutic approaches.

Concentration of Glutethimide and Associated Compounds in Human Serum and Cerebrospinal Fluid After Drug Overdose

1974 ◽  
Vol 20 (2) ◽  
pp. 195-199 ◽  
Author(s):  
Martin Gold ◽  
Ernest Tassoni ◽  
Michael Etzl ◽  
George Mathew
Keyword(s):  
Gas Chromatography ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Serum Concentration ◽  
Human Serum ◽  
Drug Overdose ◽  
Gas Liquid Chromatography ◽  
Maximum Serum ◽  
Maximum Serum Concentration ◽  
Maximal Serum Concentration

Abstract Serum and cerebrospinal fluid of patients in coma owing to glutethimide overdose was assayed for glutethimide and associated compounds. The sample was extracted with chloroform and the extract assayed by gas-liquid chromatography on a column of "3% OV-17." Up to six constituents were found in serum in addition to glutethimide, only three of which were ever present in substantial quantity. The peaks seen on gas chromatography were numbered in order of elution. Glutethimide was peak No. 2. Peak No. 1 usually reached a maximum serum concentration in 10 or less hours, as did peak No. 4. Peak No. 3 reached a maximal serum concentration in 10 h or sooner also, but at 20 h the amount had changed little in most patients. Ambre and Fischer [Res. Commun. Pathol. Pharmacol. 4, 307 (1972)] speculate that peak No. 3 plays an important role in maintaining coma. Neither the pattern of change nor the relative concentrations in serum or cerebrospinal fluid on waking support their hypothesis.

Analysis for diazepam and nordiazepam by electron-capture gas chromatography and by liquid chromatography.

1979 ◽  
Vol 25 (7) ◽  
pp. 1296-1300 ◽  
Author(s):  
J E Wallace ◽  
H A Schwertner ◽  
E L Shimek
Keyword(s):  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electron Capture ◽  
Chromatographic Analysis ◽  
High Performance ◽  
Reversed Phase ◽  
Gas Liquid Chromatography ◽  
Stationary Liquid Phase ◽  
Concurrent Analysis

Abstract We describe the use of electron-capture gas chromatography or reversed-phase "high-performance" liquid chromatography for concurrent analysis for diazepam and nordiazepam in serum. In the gas-chromatographic analysis our use of a new chemically deactivated stationary liquid phase, SP 2250-DB (Supelco, Inc.), resulted in improved chromatographic sensitivity and peak symmetry for the two benzodiazepines as compared to that obtained with either OV-17or OV-1 phases. Steady-state concentrations of diazepam and nordiazepam in serum as determined by gas-liquid chromatography correlated closely with those found by liquid-liquid chromatography.

scholarly journals Electron capture gas chromatography detection and mass spectrum identification of 3-(2'-ketohexyl)indoline in spinal fluids of patients with tuberculous meningitis

1977 ◽  
Vol 5 (6) ◽  
pp. 625-628
Author(s):  
J B Brooks ◽  
G Choudhary ◽  
R B Craven ◽  
C C Alley ◽  
J A Liddle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Mass Spectrum ◽  
Electron Capture ◽  
Tuberculous Meningitis ◽  
Gas Liquid Chromatography ◽  
Heptafluorobutyric Anhydride

A basic, extractable, indolic type of compound, which was derivatized with heptafluorobutyric anhydride and pyridine, was obtained from the cerebrospinal fluids of patients with acute tuberculous meningitis. The compound was detected by frequency-pulsed, modulated electron capture gas-liquid chromatography, and it was tentatively identified by mass spectrometry as 3-(2'-ketohexyl)indoline. The compound was found to be valuable for differentiating between tuberculous, cryptococcal, and aseptic meningitides.

scholarly journals Determination of δ-aminolaevulinic acid in biological fluids by gas-liquid chromatography with electron-capture detection

1984 ◽  
Vol 219 (3) ◽  
pp. 883-889 ◽  
Author(s):  
A Gorchein
Keyword(s):  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Electron Capture ◽  
Biological Fluids ◽  
Internal Standard ◽  
Electron Capture Detection ◽  
Gas Liquid Chromatography ◽  
Aminolaevulinic Acid ◽  
Highly Sensitive

A derivative of delta-aminolaevulinic acid (AmLev), 2-methyl-3-acetyl-4-(3-propionic acid pentafluorobenzyl ester)pyrrole, with favourable properties for g.l.c. with electron-capture detection, was synthesized. Less than 1 pg could be detected on the column. 6-Amino-5-oxohexanoic acid formed the analogous derivative under similar conditions and was used as the internal standard in the development of a highly sensitive and specific assay for AmLev. The method has been applied to peripheral-venous and umbilical-cord plasma and to cerebrospinal fluid of normal and porphyric subjects.

Citramalic acid in cerebrospinal fluid of patients with bacterial meningitis.

1984 ◽  
Vol 30 (7) ◽  
pp. 1209-1212 ◽  
Author(s):  
S Perlman ◽  
S A Carr
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Viral Meningitis ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Hydroxy Acids ◽  
Ionization Detector ◽  
Flame Ionization

Abstract Cerebrospinal fluid (CSF) from uninfected patients and from patients with bacterial and viral meningitis was analyzed by gas-liquid chromatography, with use of a flame ionization detector, and by gas chromatography-mass spectrometry. The resulting profiles were consistent and reproducible. Hydroxy acids were the compounds found in greatest abundance in both normal and infected CSF. Control experiments to establish the sensitivity and efficiency of the extraction and derivatization methods are also presented. Constituents of CSF from patients with bacterial meningitis differed quantitatively and qualitatively from those of CSF from uninfected patients or patients with nonbacterial infections. CSF from seven of eight patients with bacterial meningitis contained citramalic acid, a compound not previously identified in either normal or infected CSF. The implications of these findings are discussed.

scholarly journals Electron capture gas chromatography study of the acid and alcohol products of Clostridium septicum and Clostridium chauvoei

1976 ◽  
Vol 3 (2) ◽  
pp. 180-185
Author(s):  
J B Brooks ◽  
M J Selin ◽  
C C Alley
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Electron Capture ◽  
Metabolic Profile ◽  
Gas Liquid Chromatography ◽  
Glucose Medium ◽  
Clostridium Septicum ◽  
Metabolic Products ◽  
Clostridium Chauvoei ◽  
Animal Isolates

The metabolic products produced by several strains of Clostridium septicum obtained from patients and animals, along with strains of Clostridium chauvoei, were studied in chopped meat glucose medium by electron capture gas-liquid chromatography (EC-GLC). The strains of C. septicum and C. chauvoei were shown to comprise five different metabolic groups. Both the EC-GLC study and the O and H antigenic study performed previously showed that strains of C. septicum comprise a heterogeneous group. One type of metabolic profile was found only in strains of C. chauvoei. The O antigen types and EC-GLC metabolic types of C. septicum correlated fairly well in isolates from cancer patients but not in stock culture and animal isolates.

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