scholarly journals Novel Immunoblot Assay Using Four Recombinant Antigens for Diagnosis of Epstein-Barr Virus Primary Infection and Reactivation

1999 ◽  
Vol 37 (8) ◽  
pp. 2709-2714 ◽  
Author(s):  
M. Buisson ◽  
B. Fleurent ◽  
M. Mak ◽  
P. Morand ◽  
L. Chan ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Primary Infection ◽  
Epstein Barr Virus ◽  
Immunofluorescence Assay ◽  
Early Antigen ◽  
Immunoblot Assay ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
Virus Capsid Antigen

A new immunoblot assay, composed of four Epstein-Barr virus (EBV)-encoded recombinant proteins (virus capsid antigen [VCA] p23, early antigen [EA] p138, EA p54, and EBNA-1 p72), was compared with an immunofluorescence assay on a total of 291 sera. The test was accurate in 94.5% of cases of primary EBV infection, while an immunoglobulin G anti-VCA p23 band with strong intensity correlated with reactivation.

scholarly journals Evaluation of 12 Commercial Tests for Detection of Epstein-Barr Virus-Specific and Heterophile Antibodies

2000 ◽  
Vol 7 (3) ◽  
pp. 451-456 ◽  
Author(s):  
Anne-Lise Bruu ◽  
Reidar Hjetland ◽  
Ellen Holter ◽  
Liisa Mortensen ◽  
Olav Natås ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Blood Donors ◽  
Immunofluorescence Assay ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Capsid Antigen ◽  
Early Antigen ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

ABSTRACT Ten microbiological departments in Norway have participated in a multicenter evaluation of the following commercial tests for detection of Epstein-Barr virus (EBV)-specific and heterophile antibodies: CAPTIA Select viral capsid antigen (VCA)-M/G/EBNA (Centocor Inc.), Enzygnost anti-EBV/immunoglobulin M (IgM) and IgG (Dade Behring), Vironostika EBV VCA IgM/IgG/EBNA enzyme-linked immunosorbent assay (ELISA) (Organon Teknika), SEROFLUOR immunofluorescence assay and EBV Combi-Test (Institute Virion Ltd.), anti-EBV recombinant IgM- and IgG-early antigen/EBNA IgG ELISA (Biotest Diagnostics), EBV IgM/IgG/EBNA ELISA (Gull Laboratories), Paul-Bunnell-Davidsohn test (Sanofi Diagnostics Pasteur), Monosticon Dri-Dot (Organon Teknika), Avitex-IM (Omega Diagnostics Ltd.), Alexon Serascan infectious mononucleosis test (Alexon Biomedical Inc.), Clearview IM (Unipath Ltd.), and Cards±OS Mono (Pacific Biotech, Inc.). The test panel included sera from patients with primary EBV infection, immunocompromised patients with recent cytomegalovirus infection, healthy persons (blood donors), and EBV-seronegative persons. Among the tests for EBV-specific antibodies the sensitivity was good, with only small differences between the different assays. However, there was a greater variation in specificity, which varied between 100% (Enzygnost) and 86% (Biotest). Tests for detection of heterophile antibodies based on purified or selected antigen (Avitex, Alexon, Clearview IM, and Cards±OS Mono) were more sensitive than the Paul-Bunnell-Davidsohn and Monosticon tests.

Encephalitis in Infectious Mononucleosis: Diagnostic Considerations

PEDIATRICS ◽  
1976 ◽  
Vol 58 (6) ◽  
pp. 877-880
Author(s):  
Beverly J. Lange ◽  
Peter H. Berman ◽  
Joseph Bender ◽  
Werner Henle ◽  
John F. Hewetson
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Nuclear Antigen ◽  
Diffuse Component ◽  
Early Antigen ◽  
Barr Virus ◽  
Ebv Infection ◽  
Antibody Titers ◽  
Epstein Barr ◽  
Etiologic Diagnosis

Four atypical cases of presumed infectious mononucleosis (IM) encephalitis are presented. To establish an etiologic diagnosis, Paul-Bunnell-Davidsohn heterophil titers (PBD), antibody titers to the antigens of the Epstein-Barr virus (EBV), and oropharyngeal excretion of EBV were determined. Criteria for a primary EBV infection are (1) an antiviral capsid antigen titer of 1:160 or greater, (2) the presence of antibody to the diffuse component of the early antigen, (3) absence of antibody to the nuclear antigen, and (4) excretion of the virus from the oropharynx. Three of the four cases met these criteria; of the three, one did not have a positive heterophil titer. The fourth case turned out not to be IM; there was a positive PBD heterophil, but there was no evidence of primary EBV infection. Although the PBD heterophil is usually a reliable test to diagnosis IM, it is not always present in children, and it is sometimes nonspecifically elevated. Some EBV titers can be nonspecifically elevated as well; however, the above criteria are diagnostic of primary EBV infection.

Primary Epstein-Barr Virus Infections in Children

PEDIATRICS ◽  
1977 ◽  
Vol 59 (1) ◽  
pp. 16-21
Author(s):  
Ciro Valent Sumaya
Keyword(s):  
Epstein Barr Virus ◽  
Viral Capsid ◽  
Viral Capsid Antigen ◽  
Virus Infections ◽  
Early Antigen ◽  
Barr Virus ◽  
Ebv Infection ◽  
Antibody Titers ◽  
Epstein Barr ◽  
A Current

During a seroepidemiologic survey of a community, 13 (6.2%) of 209 children were found to be experiencing a current or recent primary Epstein-Barr virus (EBV) infection. The sera contained elevated antibody titers to viral capsid antigen of EBV, antibodies to early antigen (EA) of EBV, and specific IgM. The frequency of primary infections was highest in the first decade of life. The primary EBV infections were usually asymptomatic. The antibody to EA was directed predominantly to the R component. A heterophil antibody response was not detected.

Evaluation of Epstein-Barr Virus Indirect Immunofluorescence Assay Results

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Gorgun S ◽  
◽  
Havuz SG ◽  
Mehel D ◽  
◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Epstein Barr Virus ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Barr Virus ◽  
Ebv Infection ◽  
Study Results ◽  
Significant Difference ◽  
Child Patients ◽  
Epstein Barr

Aim: Epstein-Barr Virus (EBV) causes Infectious Mononucleosis (IM) and chronic active EBV infections, malignant diseases such as Burkitt’s lymphoma, nasopharyngeal carcinoma, and posttransplant lymphoproliferative disease. More than 90% of children are infected with EBV until the age of six. The aim of this study was to diagnose EBV infection using Indirect Immunofluorescence Assay (IIFA) method and to evaluate these patients clinically. Methods: The tests were studied by using EBV Indirect Immunofluorescence Assay (IIFA) method. A total of 247 patients, 186 adults, and 61 children, were included in the study. Results: Five (2.7%) of the adults were EBV-Capsid Antigen (EBV-CA) IgM positive, 175 (94.1%) were IgG positive and 6 (3.2%) were seronegative. 10 (16.4%) of the child patients were IgM positive, which is considered as an acute IM infection marker, whereas the child patients had a significantly higher IgM rate than adults (p <0.001). 39 (63.9%) of the child patients were IgG positive and 12 (19.7%) of them were seronegative. The rate of IgG positivity in children was significantly lower than in adults (p <0.001). There was no significant difference between the genders in terms of IgM and IgG positivity rates in both adults and children (p >0.05 for each). Conclusion: These results suggest that the presence of an acute EBV infection should be considered when the patient has viral diseases with similar clinical picture especially for children.

Infectious mononucleosis and Epstein-Barr virus

2004 ◽  
Vol 6 (23) ◽  
pp. 1-16 ◽  
Author(s):  
Eleni-Kyriaki Vetsika ◽  
Margaret Callan
Keyword(s):  
Infectious Mononucleosis ◽  
Primary Infection ◽  
Epstein Barr Virus ◽  
Latent Infection ◽  
Clinical Syndrome ◽  
Primary Immune Response ◽  
Barr Virus ◽  
Glandular Fever ◽  
Ebv Infection ◽  
Epstein Barr

Epstein-Barr virus (EBV) is a γ-herpesvirus that infects over 90% of the human population worldwide. It is usually transmitted between individuals in saliva, and establishes replicative infection within the oropharynx as well as life-long latent infection of B cells. Primary EBV infection generally occurs during early childhood and is asymptomatic. If delayed until adolescence or later, it can be associated with the clinical syndrome of infectious mononucleosis (also known as glandular fever or ‘mono%rsquo;), an illness characterised by fevers, pharyngitis, lymphadenopathy and malaise. EBV infection is also associated with the development of EBV-associated lymphoid or epithelial cell malignancies in a small proportion of individuals. This review focuses on primary EBV infection in individuals suffering from infectious mononucleosis. It discusses the mechanism by which EBV establishes infection within its human host and the primary immune response that it elicits. It describes the spectrum of clinical disease that can accompany primary infection and summarises studies that are leading to the development of a vaccine designed to prevent infectious mononucleosis.

scholarly journals Uji serologi IgA karakter KNF EBNA1+VCA p-18 pada penderita keluhan kronis kepala leher

2011 ◽  
Vol 41 (2) ◽  
pp. 105
Author(s):  
Camelia Herdini ◽  
Susanna Hutajulu ◽  
Sagung Rai Indrasari ◽  
Bambang Hariwiyanto ◽  
Jajah Fachiro ◽  
...  
Keyword(s):  
Head And Neck ◽  
Epstein Barr Virus ◽  
Viral Capsid ◽  
Viral Capsid Antigen ◽  
Early Antigen ◽  
Barr Virus ◽  
Ebv Infection ◽  
Serology Test ◽  
Epstein Barr ◽  
High Level

Background: Nasopharyngeal carcinoma (NPC), especially the WHO type III, is correlated almost100% with Epstein Barr Virus (EBV) infection. This is indicated by high IgG and IgA antibody responsesagainst viral capsid antigen (VCA), early antigen (EA) and Epstein Barr Nuclear antigen (EBNA).Increased IgA NPC character antibodies may be detected 2-10 years before the presence of the tumor. Thisoccurs as a result of reactivation of EBV infection. Purpose: To find out the level of IgA NPC characterantibodies (EBNA1+VCA p-18) in patients with chronic symptoms in the head and neck and to determine whether the level of IgA can be used as an early sign of NPC. Methods: Observational analytic study on 218 patients with chronic symptoms in the head and neck. The research was conducted from July 2006to September 2010. ELISA technique was used as serology test for IgA (EBNA1+VCA p-18). Result: Samples were 90 males and 128 females. High level of IgA by ELISA was found in 28 males (31.1%) and 45 females (35.2%). The IgA level tended to increase with age. The most common chronic symptoms inthe head and neck were chronic rhinitis (15.6%) and nasal obstruction (7.8%). From all patients who hadhigh level of IgA, 3 patients (4.1%) were found positive of early stage NPC. Conclusion: More than 33%of patients with chronic symptoms of head and neck had high level of IgA NPC character. This methodcan be used as an early detection of NPC. Keywords: serology test in NPC, EBNA1, VCA p-18, NPC symptoms in head and neck Abstrak :  Latar belakang: Karsinoma nasofaring (KNF) terutama tipe WHO III berkorelasi hampir 100%dengan infeksi Epstein Barr Virus (EBV). Hal ini ditunjukkan dengan tingginya respons antibodi IgGdan IgA terhadap viral capsid antigen (VCA), early antigen (EA) EBV serta antibodi Epstein BarrNuclear Antigen (EBNA). Kenaikan antibodi IgA dengan karakter KNF dapat terjadi 2-10 tahun sebelumterjadinya tumor. Hal ini terjadi sebagai akibat adanya reaktivasi infeksi EBV. Tujuan: Mengetahui kadarIgA karakter KNF (EBNA1+VCA p-18) pada penderita dengan gejala kronis di daerah kepala dan leherdan mengetahui apakah kadar IgA dapat digunakan sebagai tanda awal terjadinya KNF. Metode: Suatukajian analitik observasional terhadap 218 penderita dengan gejala kronis di daerah kepala dan leher.Penelitian ini dilakukan Juli 2006 sampai dengan September 2010. Pemeriksaan serologi IgA (EBNA1+VCA-p18)dilakukan denganteknik ELISA.Hasil:Terdapat90penderita laki-lakidan128 penderitaperempuan.HasiltesserologiIgAELISAdengankadartinggiditemukanpada28laki-laki(31,1%)dan45perempuan (35,2%). Kadar IgA cenderung meningkat pada peningkatan usia. Gejala kronis yangterbanyak dikeluhkan penderita adalah rinitis kronis, yaitu sebanyak 34 penderita (15,6%), diikuti denganobstruksi hidung sebanyak 17 penderita (7,8%). Pemeriksaan klinis lebih lanjut dari penderita yangmempunyai kadar IgA tinggi menunjukkan bahwa 3 penderita (4,1%) positif terkena kanker nasofaringstadium awal. __ Lebih dari 33% penderita dengan gejala kronis di daerah kepala dan lehermemiliki kadar IgA karakter KNF yang tinggi. Kadar IgA karakter KNF yang tinggi dapat digunakansebagai penanda awal kejadian KNF. Kata kunci: uji serologi KNF,EBNA1, VCA p-18, gejala KNF

Evaluation of Epstein-Barr Virus Indirect Immunofluorescence Assay Results

2020 ◽  
Author(s):  
Selim Gorgun ◽  
Seda Gudul Havuz ◽  
Dursun M. Mehel
Keyword(s):  
Indirect Immunofluorescence ◽  
Epstein Barr Virus ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Barr Virus ◽  
Ebv Infection ◽  
Significant Difference ◽  
Child Patients ◽  
Epstein Barr ◽  
Acute Infectious Mononucleosis

Abstract Background: The aim of this study was to diagnose Epstein-Barr virüs (EBV) infection using indirect immunofluorescence assay (IIFA) method and to evaluate these patients clinically. More than 90% of children are infected with EBV until the age of six. Methods: The tests were studied by using EBV IIFA method. A total of 247 patients, 186 adults, and 61 children, were included in the study. Results: 5 (2.7%) of the adults were EBV-Capsid antigen (EBV-CA) IgM positive, 175 (94.1%) were IgG positive and 6 (3.2%) were seronegative. 10 (16.4%) of the child patients were IgM positive, which is considered as an acute infectious mononucleosis (IM) infection marker, whereas the child patients had a significantly higher IgM rate than adults (p <0.001). 39 (63.9%) of the child patients were IgG positive and 12 (19.7%) of them were seronegative. The rate of IgG positivity in children was significantly lower than in adults (p <0.001).There was no significant difference between the genders in terms of IgM and IgG positivity rates in both adults and children(p> 0.05 for each).Conclusion: These results suggest that the presence of an acute EBV infection should be considered when the patient has viral diseases with similar clinical picture especially for children.

scholarly journals Total Antioxidant Status (TAS), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) in Oropharyngeal Cancer Associated with EBV Infection

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Małgorzata Strycharz-Dudziak ◽  
Małgorzata Kiełczykowska ◽  
Bartłomiej Drop ◽  
Łukasz Świątek ◽  
Ewa Kliszczewska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oropharyngeal Cancer ◽  
Epstein Barr Virus ◽  
Total Antioxidant Status ◽  
Wild Type ◽  
Early Antigen ◽  
Barr Virus ◽  
Ebv Infection ◽  
Total Antioxidant ◽  
Epstein Barr

A growing number of studies reveal that oxidative stress is associated with viral infections or cancer development. However, there are few reports assessing the relationships between oxidative stress, viral infection, and carcinogenesis. The present study analyzed the level of total antioxidant status (TAS) as well as the activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) in patients with oropharyngeal cancer both Epstein-Barr virus (EBV)-positive and EBV-negative in comparison with the control group. The correlations between these parameters and EBV type (wild-type LMP1 (wt-LMP1) or LMP1 with deletion (del-LMP1)), level of antibodies against EBV, the degree of tumor differentiation, and TNM classification were also investigated. Fresh frozen tumor tissue samples collected from 66 patients with oropharyngeal squamous cell carcinoma were tested using nested PCR assay for EBV DNA detection. Spectrophotometric methods were used to measure TAS values as well as SOD and GPx activities in homogenates of tissue, using diagnostic kits produced by Randox Laboratories. Sera from all individuals were investigated using ELISA method to detect the presence of Epstein-Barr virus capsid antigen (EBVCA) IgM and IgG, Epstein-Barr virus nuclear antigen (EBNA) IgG, and early antigen (EA) IgG antibodies. The level of TAS and activities of antioxidant enzymes (GPx and SOD) were significantly decreased in tissues with oropharyngeal cancer, particularly in EBV-positive cases. In 82.3% of patients, wt-LMP1 was detected. Significantly lower TAS, GPx, and SOD values were stated in patients infected with wild-type EBV. The presence of antibodies against early antigen (anti-EA) was detected in over 80% of patients, which suggests reactivation of EBV infection. The correlation between the degree of tumor differentiation and TN classification, especially in EBV-positive patients, was also observed. Determination of these parameters may be useful in evaluating tumor burden in patients with various stages of oropharyngeal cancer and could be an important prognostic factor. Future studies are needed to understand the role of EBV lytic reactivation induced by oxidative stress.

scholarly journals Detection of Epstein-Barr virus in autoimmune and Thalassemia patients using new Immunoblot assay

2015 ◽  
Vol 9 (1) ◽  
pp. 13-16
Author(s):  
Alaa Younis Mahdy Al-Hamadany Al-Hamadany ◽  
Basima A. Abdullah Abdullah
Keyword(s):  
Epstein Barr Virus ◽  
Acute Infection ◽  
Autoimmune Disorders ◽  
Healthy Controls ◽  
Serum Samples ◽  
Immunoblot Assay ◽  
Barr Virus ◽  
Epstein Barr ◽  
Antibody Pattern ◽  
Virus Capsid Antigen

A new immunoblot assay, composed of five Epstein-Barr virus (EBV)-encoded recombinantproteins virus capsid antigen [VCA] gp125, p19, p22, early antigen [EA], and EBNA-1 IgG, wasused to manifest the EBV infection and look at the antibody pattern to EBV proteins in the serumof both autoimmune disorders and Thalassemia patients and compare the observations with thosein normal healthy controls. Serum samples from 35 rheumatoid arthritis patients, 20 SLE, 20autoimmune hypothyroid diseases, 35 Thalassemia patients and 20 healthy controls were testedfor EBV IgG antibodies by an immunoblot assay (Euroline). The results showed that the highpercentage recorded was 50% in acute infection. Followed by 30% at late infection, while latephase with loss EBNA-1 and reactivated infection were 10% compared to the normal healthycontrols. Our study showed an increased EBV activation among the autoimmune patient groupscompared to the normal healthy controls. Further studies are required to delineate the associationbetween the etiology of autoimmune disorders and EBV.

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