scholarly journals Genetic Diversity of Histoplasma capsulatum Strains Isolated from Soil, Animals, and Clinical Specimens in Rio de Janeiro State, Brazil, by a PCR-Based Random Amplified Polymorphic DNA Assay

2001 ◽  
Vol 39 (12) ◽  
pp. 4487-4494 ◽  
Author(s):  
M. d. M. Muniz ◽  
C. V. Pizzini ◽  
J. M. Peralta ◽  
E. Reiss ◽  
R. M. Zancope-Oliveira
Keyword(s):  
Genetic Diversity ◽  
Rio De Janeiro ◽  
Histoplasma Capsulatum ◽  
Random Amplified Polymorphic Dna ◽  
Soil Animals ◽  
Clinical Specimens ◽  
Dna Assay ◽  
Janeiro State

Genetic diversity of porcine enteric caliciviruses in pigs raised in Rio de Janeiro State, Brazil

2010 ◽  
Vol 155 (8) ◽  
pp. 1301-1305 ◽  
Author(s):  
Juliana Bragazzi Cunha ◽  
Marcos César Lima de Mendonça ◽  
Marize Pereira Miagostovich ◽  
José Paulo Gagliardi Leite
Keyword(s):  
Genetic Diversity ◽  
Rio De Janeiro ◽  
Enteric Caliciviruses ◽  
Janeiro State

scholarly journals Genetic Analysis of Histoplasma capsulatum Strains Isolated from Clinical Specimens in Thailand by a PCR-Based Random Amplified Polymorphic DNA Method

1998 ◽  
Vol 36 (10) ◽  
pp. 3073-3076 ◽  
Author(s):  
Natteewan Poonwan ◽  
Tamae Imai ◽  
Nanthawan Mekha ◽  
Katsukiyo Yazawa ◽  
Yuzuru Mikami ◽  
...  
Keyword(s):  
Reference Strain ◽  
Histoplasma Capsulatum ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Primers ◽  
Dna Fingerprint ◽  
Sequence Information ◽  
Clinical Specimens ◽  
American Strain ◽  
The Difference

Thirteen strains of Histoplasma capsulatum were isolated from clinical specimens, including those from AIDS patients, in Thailand. Random amplified polymorphic DNA (RAPD) analysis with three different PCR primers showed that the DNA fingerprint patterns of the Thai isolates were very similar to each other and homogeneous, with only one exceptional strain, although the patterns were clearly different from those of a reference North American strain with all primers tested. Although the difference in the DNA fingerprinting patterns was minor, Thai isolates could be classified into two to four groups. A common PCR band (about 700 bp) in the patterns of allH. capsulatum strains was extracted, and its DNA sequence was determined. A new PCR primer set for the identification of H. capsulatum species was developed based on this sequence information. This primer set was 100% successful in the identification of the reference strain as well as all Thai isolates. The results of specificity tests of the primer set for the identification of the fungus are also discussed.

scholarly journals Using RAPD Markers to Assess Genetic Diversity in Brassica Germplasm and Chinese Breeding Lines

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 840C-840
Author(s):  
Anfu Hou ◽  
James R. McFerson ◽  
Warren F. Lamboy
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Germplasm Conservation ◽  
Breeding Lines ◽  
Dna Assay ◽  
Diversity Assessment ◽  
Average Similarity

Molecular DNA markers based on the RAPD (random amplified polymorphic DNA) assay are gaining use in germplasm assessment. RAPD markers are simple, relatively inexpensive, and highly informative. We used five primers to assess 26 Brassica oleracea breeding lines from the IVF and nine accessions from the PGRU. The test array included eight subspecies of B. oleracea. We generated 90 RAPD markers and were able to unambiguously discriminate among all 35 test entries, but could not separate subspecies within B. oleracea. Genetic similarity between subspecies ranged from 0.629 to 0.738. Average similarity within accessions was 0.96, confirming the suspected homogeneity of breeding lines. Nevertheless, significant genetic diversity was found among kohlrabi, broccoli, and cabbage accessions. Similarity analysis of breeding lines and hybrids confirmed their pedigree relationships. Interestingly, B. o. subsp. costata `Couve Nabica' showed closer similarity to B. napus subsp. oleifera `Jet Neuf' than to other B. o. materials and B. o. subsp. italica `Packman' showed higher similarity to some cabbages than to other broccolis. Results provide further evidence that diversity assessment using RAPDs is broadly applicable and useful in germplasm conservation and utilization.

scholarly journals High Uranium Concentrations in the Groundwater of the Rio de Janeiro State, Brazil, Mountainous Region

2018 ◽  
Author(s):  
José Godoy ◽  
Paulo Ferreira ◽  
Elder de Souza ◽  
Larisse da Silva ◽  
Isabela Bittencourt ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Mountainous Region ◽  
Janeiro State

A new species of Gonyleptellus Roewer, 1930 from the mountains of Rio de Janeiro State, Brazil (Opiliones: Gonyleptidae)

2020 ◽  
Vol 54 (29-30) ◽  
pp. 1947-1956
Author(s):  
Ludson Neves de Ázara ◽  
Miguel Medrano ◽  
Adriano Brilhante Kury
Keyword(s):  
New Species ◽  
Rio De Janeiro ◽  
A New Species ◽  
Janeiro State

scholarly journals Características sociodemográficas e clínicas de pacientes portadoras de cardiomiopatia periparto: contribuições para a enfermagem [Sociodemographic and clinical characteristics of peripartum cardiomyopathy patients: contributions to nursing] [Características sociodemográficas y clínicas de pacientes portadoras de cardiomiopatía periparto: contribuciones a la enfermería]

2018 ◽  
Vol 26 ◽  
pp. e32033
Author(s):  
Tamires Grama dos Santos ◽  
Karla Biancha Silva de Andrade ◽  
Flávia Giron Camerini ◽  
Andrezza Serpa Franco ◽  
Ana Lúcia Cascardo Marins ◽  
...  
Keyword(s):  
Skin Color ◽  
Rio De Janeiro ◽  
Clinical Characteristics ◽  
Peripartum Cardiomyopathy ◽  
University Hospital ◽  
Respiratory Pattern ◽  
Cross Sectional ◽  
Documentary Analysis ◽  
Cardiac Intensive Care ◽  
Janeiro State

Objetivo: identificar as características clínicas e sociodemográficas das pacientes portadoras de cardiomiopatia periparto em unidade cardiointensiva de um hospital universitário no Estado do Rio de Janeiro. Método: estudo quantitativo, transversal, de análise documental em cinco prontuários, entre janeiro de 2014 a dezembro de 2016, obedecendo aos critérios preestabelecidos de inclusão e exclusão e iniciado após aprovação do Comitê de Ética e Pesquisa. Os dados foram coletados mediante um formulário próprio, tabulados em planilhas eletrônicas e analisados através de estatística descritiva simples. Resultados: houve predominância de idade entre 26-35 anos – 4 (80%), multíparas, etnia parda, internação para pós-operatório de parto cesárea – 4 (80%), internação inferior a 2 semanas 4 (80%). Dentre os problemas de enfermagem encontrados, estão o débito cardíaco diminuído e padrão respiratório ineficaz. Conclusão: divulgar as características sociodemográficas e clínicas dessa clientela pode ajudar a delimitar alguns problemas de enfermagem representativos nessa população.ABSTRACTObjective: to identify the clinical and sociodemographic characteristics of patients with peripartum cardiomyopathy in a cardiac intensive care unit of a university hospital in Rio de Janeiro State. Method: this quantitative, cross-sectional, documentary analysis studied five medical records, between January 2014 and December 2016, according to pre-established inclusion and exclusion criteria, after approval by the research ethics committee. Data were collected using a specific form, tabulated in electronic spreadsheets, and analyzed by simple descriptive statistics. Results: subjects were aged predominantly 26-35 years (4; 80%), multiparous, skin color brown, admitted for caesarean section postoperative care (4; 80%), and for less than 2 weeks (4; 80%). The nursing problems encountered included decreased cardiac output and ineffective respiratory pattern. Conclusion: disclosure of this clientele’s sociodemographic and clinical characteristics can help to delimit some representative nursing problems in this population.RESUMENObjetivo: identificar las características clínicas y sociodemográficas de las pacientes portadoras cardiomiopatía periparto en una unidad de cuidados intensivos coronarios de un hospital universitario en el Estado de Río de Janeiro. Método: estudio cuantitativo, transversal, de análisis documental en cinco historias clínicas, entre enero de 2014 y diciembre de 2016, obedeciendo a los criterios preestablecidos de inclusión y exclusión e iniciado tras la aprobación del Comité de Ética en Investigación. Los datos fueron recolectados a través de un formulario propio, tabulados en planillas electrónicas y analizados a través de estadística descriptiva simple Resultados: hubo predominancia de edad entre 26 y 35 años – 4 (80%), multíparas, etnia parda, internación para postoperatorio de parto cesárea - 4 (80%), internación inferior a 2 semanas – 4 (80%). Entre los problemas de enfermería encontrados, están el débito cardíaco disminuido y el patrón respiratorio ineficaz. Conclusión: divulgar las características sociodemográficas y clínicas de esa clientela puede ayudar a delimitar algunos problemas de enfermería representativos en esa población.

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