scholarly journals Analysis of Mycobacterium avium Complex Isolates from Blood Samples of AIDS Patients by Pulsed-Field Gel Electrophoresis

2003 ◽  
Vol 41 (1) ◽  
pp. 498-499 ◽  
Author(s):  
D. Y. Kunimoto ◽  
M. S. Peppler ◽  
J. Talbot ◽  
P. Phillips ◽  
S. D. Shafran
Keyword(s):  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Mycobacterium Avium Complex ◽  
Pulsed Field Gel Electrophoresis ◽  
Aids Patients ◽  
Blood Samples ◽  
Pulsed Field

scholarly journals Genetic characterization ofMycobacterium aviumisolates recovered from humans and animals in Australia

1996 ◽  
Vol 116 (1) ◽  
pp. 41-49 ◽  
Author(s):  
M. M. Feizabadi ◽  
I. D. Robertson ◽  
D. V. Cousins ◽  
D. Dawson ◽  
W. Chew ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Type ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

SummaryGenetic relationships amongst 115 mainly Australian isolates ofMycobacterium aviumwere assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0·29. Isolates from humans were closely related to but distinct from those cultured from birds, whilst some porcine isolates belonged to the same ETs as certain human isolates. Pulsed field gel electrophoresis (PFGE) was used to differentiate related isolates, and those from birds and some from other animals, including pigs, were distinguished from the human isolates. The results of MEE and PFGE suggested that certain strains ofM. aviummay be transmitted between birds and pigs, but there was no clear evidence of transmission to humans. The serovar of theM. aviumisolates was not obviously related to their ET assignment or their PFGE type.

scholarly journals Molecular epidemiology of Mycobacterium avium complex isolated from patients with and without AIDS in Brazil and England

1999 ◽  
Vol 122 (3) ◽  
pp. 435-440 ◽  
Author(s):  
M. A. S. TELLES ◽  
M. D. YATES ◽  
M. CURCIO ◽  
S. Y. M. UEKI ◽  
M. PALACI ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Mycobacterium Avium Complex ◽  
Opportunistic Pathogen ◽  
Pulsed Field Gel Electrophoresis ◽  
The World ◽  
Unique Strain ◽  
Over Time ◽  
Hiv Negative

Mycobacterium avium complex (MAC) is ubiquitous throughout the world. It is an opportunistic pathogen in AIDS patients but the number of cases in HIV negative patients is also increasing. The aim of this study was to determine whether patients were being infected with different MAC strains or whether one strain was dominant. DNA obtained from isolates in Brazil and England were compared using pulsed field gel electrophoresis (PFGE). Strains from 22 Brazilian patients clustered into 7 groups but 68/90 patients had a unique strain. In all patients, Brazilian and English, the same strain was isolated repeatedly over time, some over several years. This study shows that it is most likely that Man is infected from the environment and that one strain can survive without change for many years both in the environment and in Man.

scholarly journals Molecular Analysis of Mycobacterium avium Isolates by Using Pulsed-Field Gel Electrophoresis and PCR

1999 ◽  
Vol 37 (8) ◽  
pp. 2450-2455 ◽  
Author(s):  
Martine Pestel-Caron ◽  
Gabriel Graff ◽  
Gilles Berthelot ◽  
Jean-Louis Pons ◽  
Jean-François Lemeland
Keyword(s):  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Insertion Sequence ◽  
Genetic Relationships ◽  
Pcr Amplification ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Copy Numbers ◽  
Region Ii ◽  
Laboratory Contamination

Genetic relationships among 46 isolates of Mycobacterium avium recovered from 37 patients in a 2,500-bed hospital from 1993 to 1998 were assessed by pulsed-field gel electrophoresis (PFGE) and PCR amplification of genomic sequences located between the repetitive elements IS1245 and IS1311. Each technique enabled the identification of 27 to 32 different patterns among the 46 isolates, confirming that the genetic heterogeneity ofM. avium strains is high in a given community. Furthermore, this retrospective analysis of sporadic isolates allowed us (i) to suggest the existence of two remanent strains in our region, (ii) to raise the question of the possibility of nosocomial acquisition ofM. avium strains, and (iii) to document laboratory contamination. The methods applied in the present study were found to be useful for the typing of M. avium isolates. In general, both methods yielded similar results for both related and unrelated isolates. However, the isolates in five of the six PCR clusters were distributed among two to three PFGE patterns, suggesting that this PCR-based method may have limitations for the analysis of strains with low insertion sequence copy numbers or for resolution of extended epidemiologic relationships.

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