scholarly journals Determination of Amoebicidal Activities of Multipurpose Contact Lens Solutions by Using a Most Probable Number Enumeration Technique

2003 ◽  
Vol 41 (7) ◽  
pp. 2992-3000 ◽  
Author(s):  
T. K. Beattie ◽  
D. V. Seal ◽  
A. Tomlinson ◽  
A. K. McFadyen ◽  
A. M. Grimason
Keyword(s):  
Contact Lens ◽  
Most Probable Number ◽  
Probable Number ◽  
Contact Lens Solutions

scholarly journals Genus Alga pada Lahan Sawah Organik yang Ditanami Padi Lokal dan Inhibrida di Subak Jatiluwih, Tabanan

2019 ◽  
Vol 9 (2) ◽  
pp. 112
Author(s):  
SAWIDYA FITRIYANI ◽  
I WAYAN DANA ATMAJA ◽  
NI NENGAH SONIARI
Keyword(s):  
Soil Fertility ◽  
Research Method ◽  
Quantitative Method ◽  
Rice Fields ◽  
Most Probable Number ◽  
Water Sampling ◽  
Probable Number ◽  
Organic Rice ◽  
Soil And Water

Algae Genus in Organic Rice Fields Planted with Local and Inhibrida Rice in Subak Jatiluwih, Tabanan. The abundance of microalgae in rice fields, especially microalgae from Cyanophyta division that are capable of fixing nitrogen, is very important helping maintain soil fertility. The aim of this study was to find out the genus of algae in organic rice fields of Subak Jatiluwih, Tabanan. The research was conducted from September 2018 to April 2019. Sampling was done in Subak Jatiluwih. Algae breeding were carried out at Biology and Physics Laboratory, Faculty of Agriculture, Udayana University. This research method uses descriptive quantitative. The implementation of the research included the determination of location, soil and water sampling, identification of algae, and analysis of algae genera. Enrichment algae was carried out using the MPN method, results of the analysis of algae genus were found in 37 genera from 3 divisions i.e Chlorophyta, Cyanophyta and Chrysophyta. Descriptive quantitative method Most Probable Number (MPN) calculation conclude that there are not much different from the Local and Inhibrida rice fields. The maximum population algae of local rice fields is 1.100 cell g-1 and Inhibrida rice fields is >1.100 cell g-1 of land. The minimum population is 3,6 cell g-1 of land in local rice fields, and 9,2 cell g-1 of land in Inhibrida rice fields.

scholarly journals GENOMIC DETERMINATION OF THE MOST IMPORTANT FATHER LINES OF SLOVAK PINZGAU COWS

AGROFOR ◽  
2016 ◽  
Vol 1 (3) ◽  
Author(s):  
Veronika KUKUČKOVÁ ◽  
Nina MORAVČÍKOVÁ ◽  
Radovan KASARDA
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
The State ◽  
Most Probable Number ◽  
Pedigree Data ◽  
Probable Number ◽  
Expected Heterozygosity ◽  
High Level ◽  
The Bayesian Approach

The aim of this study was to assess genetic structure of Slovak Pinzgau populationbased on polymorphism at molecular markers using statistical methods. Femaleoffspring of 12 most frequently used bulls in Slovak Pinzgau breeding programmewere investigated. Pinzgau cattle were found to have a high level of diversity,supported by the number of alleles observed across loci (average 5.31, range 2-11)and by the high within-breed expected heterozygosity (average 0.66, range 0.64-0.73). The state of genetic diversity is satisfying and standard for local populations.Detection of 12 possible subpopulation structures provided us with detailedinformation of the genetic structure. The Bayesian approach was applied, detectingthree, as the most probable number of clusters. The similarity of eachsubpopulation using microsatellites was confirmed also by high-throughputmolecular data. The observed inbreeding (FROH=2.3%) was higher than thatexpected based on pedigree data (FPED=0.4%) due to the limited number ofavailable generations in pedigree data. One of the most important steps indevelopment of efficient autochthonous breed protection programs ischaracterization of genetic variability and assessment of the population structure.The chosen set of microsatellites confirmed the suitability in determination of thesubpopulations of Pinzgau cattle in Slovakia. The state of genetic diversity at moredetailed level was successfully performed using bovineSNP50 BeadChip.

Determination of benzalkonium chloride in contact lens solutions by positive-ion fast atom bombardment mass spectrometry

1989 ◽  
Vol 7 (12) ◽  
pp. 1611-1616 ◽  
Author(s):  
S. Pinzauti ◽  
M. Bambagiotti-Alberti ◽  
G. Moneti ◽  
E. La Porta ◽  
S.A. Coran ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Contact Lens ◽  
Benzalkonium Chloride ◽  
Fast Atom Bombardment ◽  
Contact Lens Solutions ◽  
Positive Ion

scholarly journals Determination of the biodegradability of chitosan utilizing the most probable number technique

2020 ◽  
Vol 42 ◽  
pp. e52965
Author(s):  
Wender Cardoso Silva ◽  
Ilva de Fátima Souza ◽  
Vivian Machado Benassi ◽  
Juan Pedro Bretas Roa ◽  
Paulo Henrique Graziotti ◽  
...  
Keyword(s):  
Soil Microorganisms ◽  
Culture Medium ◽  
Culture Media ◽  
Soil Samples ◽  
Most Probable Number ◽  
Probable Number ◽  
Strong Indicator ◽  
Specific Culture ◽  
Chitosan Biopolymer

The present work aimed to evaluate the degradability of the chitosan polymer by soil microorganisms. This evaluation was accomplished using the Most Probable Number (MPN) method by plating in drops so that soil microorganisms capable of degrading the polymeric material could be quantified. Soil samples diluted in three specific culture media for each type of microorganism were plated – bacteria, fungi and actinobacteria – and they were maintained at 28°C for seven days to determine the growth rate of fungi and actinobacteria, and for 48 hours for the development of bacteria. Significant differences in the MPN of actinobacteria relative to the other groups analyzed were observed. Thus, the method used was effective for determining the degradability of the chitosan biopolymer when observing the development of microorganisms subjected to the replacement of the carbon source by the addition of 2% w v-1 of the chitosan biopolymer to the culture medium. The formation of clear regions around the microbial colonies was a strong indicator of biodegradation.

Enumeration of petroleum-degrading marine and estuarine microorganisms by the most probable number method

1978 ◽  
Vol 24 (5) ◽  
pp. 552-557 ◽  
Author(s):  
A. L. Mills ◽  
C. Breuil ◽  
R. R. Colwell
Keyword(s):  
Crude Oil ◽  
Liquid Medium ◽  
Silica Gel ◽  
Agar Medium ◽  
Most Probable Number ◽  
Probable Number ◽  
Petroleum Degraders

Several media designed for use in a most probable number (MPN) determination of petroleum-degrading microorganisms were compared. The best results, i.e., largest numbers, were obtained using a buffered (32 mM PO4≡) liquid medium containing 1% hydrocarbon substrate. Of 104 presumptive oil degraders tested, 20 grew on oil agar medium but did not utilize oil or a mixture of pure paraffinic hydrocarbons (C10 to C16n-alkanes) in liquid (MPN) medium. Visible turbidity in the liquid medium was correlated with hydrocarbon utilization. Counts of petroleum degraders obtained using liquid medium (MPN) were in most cases higher than those obtained on an oil-amended silica gel medium. Both procedures yield an estimation of oil degraders, and the oil-amended agar permits growth of organisms which do not degrade crude oil. All strains of oil-degrading microorganisms examined in this study were lipolytic, but the converse was not always true.

Relative Effectiveness of Selenite Cystine Broth, Tetrathionate Broth, and Rappaport-Vassiliadis Medium for the Recovery of Salmonella from Raw Flesh and Other Highly Contaminated Foods: Precollaborative Study

1995 ◽  
Vol 78 (2) ◽  
pp. 375-380 ◽  
Author(s):  
Geraldine A June ◽  
Patricia S Sherrod ◽  
Thomas S Hammack ◽  
R Miguel Amaguana ◽  
Wallace H Andrews
Keyword(s):  
Relative Effectiveness ◽  
Ground Beef ◽  
Most Probable Number ◽  
Salmonella Spp ◽  
Probable Number ◽  
Pork Sausage ◽  
Selective Enrichment

Abstract The effectiveness of selenite cystine (SC) broth, tetrathionate (TT) broth, and Rappaport-Vassiliadis (RV) medium for recovery of Salmonella spp. from 8 highly contaminated foods was determined. RV medium prepared from individual ingredients and incubated at 42° and 43°C was compared with 2 commercial (Difco and Oxoid) media incubated at 42°C. Naturally and artificially contaminated foods were tested under 2 protocols. For Protocol 1, each food was preenriched in the appropriate medium. After incubation, serial 10 fold dilutions of the preenriched foods were inoculated into selective enrichment media and incubated at 35°, 42°, or 43°C. Effectiveness of these conditions was evaluated by most probable number determination of Salmonella spp. recovered. Productivity of selective enrichments did not differ significantly with this protocol, except that with Oxoid RV medium the number of Salmonella cells recovered from most of the foods was significantly reduced. For Protocol 2, twenty 25 g test portions from artificially inoculated foods were examined qualitatively for Salmonella spp. The effectiveness of the broth/temperature combinations was determined by the number of positive tests under each condition. RV medium prepared from individual ingredients and TT broth incubated at 43°C yielded significantly more Salmonella-positive tests from frog legs and lettuce than did SC and TT broths incubated at 35°C or commercial RV medium incubated at 42°C. With pork sausage and ground beef, significantly fewer Salmonella-positive tests were found with Oxoid RV medium incubated at 42°C and SC incubated at 35°C than from other selective enrichments. With chicken, fewer Salmonella-positive tests were found from SC and TT broths incubated at 35°C and Oxoid RV medium incubated at 42°C than from other selective enrichments. There were no significant differences among selective enrichments in the recovery of Salmonella spp. from the remaining foods. Overall, RV prepared from individual ingredients and incubated at 42°C yielded the highest number of Salmonella-posWive tests.

ICMSF Methods Studies. XI. Collaborative/Comparative Studies on Determination of Coliforms Using the Most Probable Number Procedure1

1979 ◽  
Vol 42 (8) ◽  
pp. 638-644 ◽  
Author(s):  
J. H. SILLIKER ◽  
D. A. GABIS ◽  
A. MAY
Keyword(s):  
Comparative Studies ◽  
Confidence Limit ◽  
Collaborative Study ◽  
The Other ◽  
Most Probable Number ◽  
Probable Number ◽  
Collaborative Studies ◽  
Different Types ◽  
Confirmatory Tests

Results of two international collaborative studies on the MPN technique for determination of coliforms in foods are reported. Three methods involving use of different presumptive and confirmatory media were compared. Results of one collaborative study conducted among 15 laboratories using eight different types of inoculated foods showed differences among the laboratories as great as 3.3 log units. The greatest difference between confirmatory tests using different media was 0.5 log units. Results of the other collaborative study conducted among five laboratories using three types of naturally contaminated foods showed differences among the laboratories as great as 1.4 log units. The greatest difference between tests using different media was 0.2 log unit. Both studies showed that the 95% confidence limit for a single value reported by a given laboratory was ± 1 log unit or ± 0.45 log unit for a mean of five values. The second study showed that a major source of variation within laboratories was between replicate aliquots. The findings are discussed in terms of their significance with respect to the monitoring of microbiological specifications for food.

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