DETERMINATION OF COLIFORMS AND ESCHERICHIA COLI IN SOME MEAT PRODUCTS USING MOST PROBABLE NUMBER TECHNIQUE

Between November 1992 and February 1993, a large outbreak of Escherichia coli O157[ratio ]H7 infections occurred in the western USA and was associated with eating ground beef patties at restaurants of one fast-food chain. Restaurants that were epidemiologically linked with cases served patties produced on two consecutive dates; cultures of recalled ground beef patties produced on those dates yielded E. coli O157[ratio ]H7 strains indistinguishable from those isolated from patients, confirming the vehicle of illness. Seventy-six ground beef patty samples were cultured quantitatively for E. coli O157[ratio ]H7. The median most probable number of organisms was 1·5 per gram (range, <0·3–15) or 67·5 organisms per patty (range, <13·5–675). Correlation of the presence of E. coli O157[ratio ]H7 with other bacterial indicators yielded a significant association between coliform count and the presence of E. coli O157[ratio ]H7 (P=0·04). A meat traceback to investigate possible sources of contamination revealed cattle were probably initially colonized with E. coli O157[ratio ]H7, and that their slaughter caused surface contamination of meat, which once combined with meat from other sources, resulted in a large number of contaminated ground beef patties. Microbiological testing of meat from lots consumed by persons who became ill was suggestive of an infectious dose for E. coli O157[ratio ]H7 of fewer than 700 organisms. These findings present a strong argument for enforcing zero tolerance for this organism in processed food and for markedly decreasing contamination of raw ground beef. Process controls that incorporate microbiological testing of meat may assist these efforts.

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Mechanically Ventilated Broiler Sheds: a Possible Source of Aerosolized Salmonella, Campylobacter, and Escherichia coli

Applied and Environmental Microbiology ◽

10.1128/aem.01380-09 ◽

2009 ◽

Vol 75 (23) ◽

pp. 7417-7425 ◽

Cited By ~ 43

Author(s):

H. N. Chinivasagam ◽

T. Tran ◽

L. Maddock ◽

A. Gale ◽

P. J. Blackall

Keyword(s):

Escherichia Coli ◽

Most Probable Number ◽

Production Cycle ◽

Airborne Bacteria ◽

Probable Number ◽

Mechanically Ventilated ◽

The Public ◽

E Coli ◽

Poultry Farms ◽

Low Levels

ABSTRACT This study assessed the levels of two key pathogens, Salmonella and Campylobacter, along with the indicator organism Escherichia coli in aerosols within and outside poultry sheds. The study ranged over a 3-year period on four poultry farms and consisted of six trials across the boiler production cycle of around 55 days. Weekly testing of litter and aerosols was carried out through the cycle. A key point that emerged is that the levels of airborne bacteria are linked to the levels of these bacteria in litter. This hypothesis was demonstrated by E. coli. The typical levels of E. coli in litter were ∼108 CFU g−1 and, as a consequence, were in the range of 102 to 104 CFU m−3 in aerosols, both inside and outside the shed. The external levels were always lower than the internal levels. Salmonella was only present intermittently in litter and at lower levels (103 to 105 most probable number [MPN] g−1) and consequently present only intermittently and at low levels in air inside (range of 0.65 to 4.4 MPN m−3) and once outside (2.3 MPN m−3). The Salmonella serovars isolated in litter were generally also isolated from aerosols and dust, with the Salmonella serovars Chester and Sofia being the dominant serovars across these interfaces. Campylobacter was detected late in the production cycle, in litter at levels of around 107 MPN g−1. Campylobacter was detected only once inside the shed and then at low levels of 2.2 MPN m−3. Thus, the public health risk from these organisms in poultry environments via the aerosol pathway is minimal.

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Microbiological counting in lamb carcasses from an abattoir in São Paulo, Brazil

Ciência Rural ◽

10.1590/s0103-84782009000600030 ◽

2009 ◽

Vol 39 (6) ◽

pp. 1836-1841 ◽

Cited By ~ 3

Author(s):

Thaís Mioto Martineli ◽

Oswaldo Durival Rossi Junior ◽

Natacha Deboni Cereser ◽

Marita Vedovelli Cardozo ◽

Cristianne Lino Fontoura ◽

...

Keyword(s):

Escherichia Coli ◽

Microbiological Quality ◽

Sao Paulo ◽

Most Probable Number ◽

São Paulo ◽

Probable Number ◽

Lamb Meat ◽

Thermotolerant Coliforms ◽

Sanitary Conditions

The consumption of lamb meat in Brazil has increased in the last years but little information about the microbiological quality of this product is available. To evaluate the hygienic-sanitary conditions of lamb carcasses, the quantification of microorganism populations indicators (mesophiles and psychrotrophs; total and thermotolerant coliforms; Escherichia coli; moulds and yeasts) and the pathogenic microorganisms indentification (Salmonella sp. and Listeria spp.) were performed. A total of 60 lamb carcasses were sampled from one abattoir in São Paulo. Swab samples were collected from three points (forequarter, back and hindquarter) on the muscle surface after carcasses final washing. Statistical analysis consisted of descriptive evaluation of the results whose counts were grouped by intervals of microorganism populations. Counts ranged from 1.0 x 10¹ to 8.0 x 10(4) colony-forming unit cm-2 (CFU cm-2) for mesophiles; 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for psychrotrophs; < 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for moulds and yeasts; < 0.3 to > 32.0 most probable number/cm² (MPN cm-2) for total and thermotolerant coliforms and Escherichia coli. Salmonella sp. and Listeria spp. were not found in any of the carcasses. Most carcasses presented low counts for all microorganisms. Overall results may be explained by the small size of the industry where the study was taken. Results suggest that good microbiological quality lamb meat is possible to be obtained, but improvement in hygienic-sanitary conditions is still required.

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Determination of Amoebicidal Activities of Multipurpose Contact Lens Solutions by Using a Most Probable Number Enumeration Technique

Journal of Clinical Microbiology ◽

10.1128/jcm.41.7.2992-3000.2003 ◽

2003 ◽

Vol 41 (7) ◽

pp. 2992-3000 ◽

Cited By ~ 61

Author(s):

T. K. Beattie ◽

D. V. Seal ◽

A. Tomlinson ◽

A. K. McFadyen ◽

A. M. Grimason

Keyword(s):

Contact Lens ◽

Most Probable Number ◽

Probable Number ◽

Contact Lens Solutions

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GREEN SYNTHESIS OF MAGNETIC IRON NANOPARTICLES USING MEDICINAL PLANT TRIDAX PROCUMBENS LEAF EXTRACTS AND ITS APPLICATION AS AN ANTIMICROBIAL AGENT AGAINST E. COLI

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020.v12s4.40102 ◽

2020 ◽

pp. 34-39

Author(s):

YOJANA Y. PATIL ◽

VAISHNVI B. SUTAR ◽

ARPITA P. TIWARI

Keyword(s):

Escherichia Coli ◽

Magnetic Nanoparticles ◽

Iron Nanoparticles ◽

Most Probable Number ◽

Leaf Extracts ◽

Probable Number ◽

Gram Negative ◽

E Coli ◽

Tridax Procumbens ◽

Escherichia Coli Bacteria

Objective: The present study was aimed at the biological synthesis of magnetic iron nanoparticles by using the plant extract of Tridax procumbens and also to study their antimicrobial property against gram-negative bacteria (Escherichia coli). Methods: The synthesis of magnetic iron nanoparticles was carried out by the co-precipitation method using biological methods like plant extract as reducing agent and capping agents are biocompatible and non-hazardous. These nanoparticles were characterized by UV-Visible spectroscopy, XRD (X-Ray Diffraction), and SEM (Scanning Electron Microscope). As well as antibacterial activity of the nanoparticles was carried out by agar well diffusion method and Most Probable Number (MPN) method against gram-negative E. coli (Escherichia coli) bacteria. Results: The average crystallite size of Magnetic Nanoparticles (MNPs) was found to be 72 nm by X-ray diffraction. The optical absorption band at wavelengths of 240 nm and 402 nm was obtained from the UV Visible spectrum. Spherical shape morphology was observed in SEM studies. The antibacterial assay clearly expressed that E. coli showed a maximum zone of inhibition (15±0.15 mm) at 2 mg/ml and 1 mg/ml concentration was found for Magnetic Nanoparticles. In the Most Probable Number (MPN) test it is seen that the bacterial count is reduced after adding synthesized NPs into the water sample. Conclusion: The results of the present study conclude that the Magnetic Nanoparticles synthesized using Tridax procumbens leaf extracts is found to be stable and show good antibacterial activity against gram-negative (Escherichia coli) bacteria.

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Genus Alga pada Lahan Sawah Organik yang Ditanami Padi Lokal dan Inhibrida di Subak Jatiluwih, Tabanan

Agrotrop : Journal on Agriculture Science ◽

10.24843/ajoas.2019.v09.i02.p02 ◽

2019 ◽

Vol 9 (2) ◽

pp. 112

Author(s):

SAWIDYA FITRIYANI ◽

I WAYAN DANA ATMAJA ◽

NI NENGAH SONIARI

Keyword(s):

Soil Fertility ◽

Research Method ◽

Quantitative Method ◽

Rice Fields ◽

Most Probable Number ◽

Water Sampling ◽

Probable Number ◽

Organic Rice ◽

Soil And Water

Algae Genus in Organic Rice Fields Planted with Local and Inhibrida Rice in Subak Jatiluwih, Tabanan. The abundance of microalgae in rice fields, especially microalgae from Cyanophyta division that are capable of fixing nitrogen, is very important helping maintain soil fertility. The aim of this study was to find out the genus of algae in organic rice fields of Subak Jatiluwih, Tabanan. The research was conducted from September 2018 to April 2019. Sampling was done in Subak Jatiluwih. Algae breeding were carried out at Biology and Physics Laboratory, Faculty of Agriculture, Udayana University. This research method uses descriptive quantitative. The implementation of the research included the determination of location, soil and water sampling, identification of algae, and analysis of algae genera. Enrichment algae was carried out using the MPN method, results of the analysis of algae genus were found in 37 genera from 3 divisions i.e Chlorophyta, Cyanophyta and Chrysophyta. Descriptive quantitative method Most Probable Number (MPN) calculation conclude that there are not much different from the Local and Inhibrida rice fields. The maximum population algae of local rice fields is 1.100 cell g-1 and Inhibrida rice fields is >1.100 cell g-1 of land. The minimum population is 3,6 cell g-1 of land in local rice fields, and 9,2 cell g-1 of land in Inhibrida rice fields.

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Identifikasi Bakteri Escherichia coli (E.coli) pada Air Minum di Rumah Makan dan Cafe di Kelurahan Jati serta Jati Baru Kota Padang

Jurnal Kesehatan Andalas ◽

10.25077/jka.v7i2.804 ◽

2018 ◽

Vol 7 (2) ◽

pp. 212

Author(s):

Wahyu Zikra ◽

Arni Amir ◽

Andani Eka Putra

Keyword(s):

Escherichia Coli ◽

Most Probable Number ◽

Probable Number

Air merupakan komponen yang sangat penting dalam kehidupan, seperti penggunanaan air sebagai air minum. Air minum juga digunakan oleh pedagang makanan. Tingginya kasus diare di Kelurahan Jati dan Jati Baru Kota Padang dapat dipicu penggunaan air minum yang tidak bersih. Tujuan penelitian ini adalah menentukan adanya kontaminasi oleh bakteri Escherichia coli dalam air minum pada rumah makan dan cafe di Kelurahan Jati dan Jati baru kota Padang. Penelitian ini bersifat deskriptif dengan perlakuan eksperimental terhadap 16 sampel air minum di Kelurahan Jati dan Jati Baru Kota Padang. Pengambilan sampel secara langsung dengan botol steril. Analisis data dengan menggunakan tabel (Most Probable Number) MPN 5-1-1 dan ditemukannya koloni bakteri Escherichia coli pada sampel air minum. Hasil penelitian ini didapatkan 13 sampel adanya kontaminasi air terhadap bakteri Coliform dengan indeks MPN didapatkan 3 sampel menunjukkan nilai indeks MPN yang tinggi yaitu: 2 sampel dengan indeks MPN 240/100 ml dan 1 sampel dengan indeks MPN 98/100 ml. Berdasarkan pemeriksaan Mac Conkey Agar ditemukan nya 13 sampel terdapat koloni bakteri Escherichia coli.

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